Thumbnail Image

Lala Lajpat Rai University of Veterinary & Animal Sciences, Hisar

Browse

20161
Reset filters
Subject: Plant Pathology ×
Reset filters

Search Results

Now showing 1 - 1 of 1
  • Thumbnail Image
    ThesisItemRestricted
    Pathological and Immunological studies on escherichia coli infection in broiler chickens fed on withania somnifera and aloe vera extracts
    (LUVAS, 2016) Mamta Kumari; Gupta, R. P.
    The present study was undertaken to investigate in vitro antibacterial activity of Withania somnifera root (WSR) and Aloe vera leaves (AVL) extracts against Escherichia coli and to study the effects of these extracts (20%WSR @ 20 ml/liter and AVL @ 20ml/liter in water) in broiler chicks (180, day old) experimentally infected with E. coli O78 @ 107CFU/0.5 ml intraperitoneally. The in vitro study revealed that the minimum inhibitory concentration against E. coli was1:16 dilution of (20% aqueous) WSR extract and 1:512 dilution of 5% AVL extract. Clinical signs of colibacillosis observed in chicks were dullness, depression, anorexia, ruffled feathers, closing of eyes, drooping of head, neck and wings, respiratory distress, watery diarrhea and dehydration. These clinical signs were mild and lasted for short duration in WSR extract supplemented infected group. Mortality due to E. coli infection was less in WSR extract supplemented group. No difference in clinical signs and mortality was observed in AVL extract supplemented infected group as compared to control infected group. A significant increase in serum alanine transaminase, aspartate transaminase, lactate dehydrogenase and creatine phosphokinase activities and a decrease in total protein and albumin concentrations was observed in the infected groups, though this increase was of lower magnitude in extract supplemented groups. However restoration of the activity of these enzymes was more evident in WSR extract supplemented group. A significant decrease in the activity of blood superoxide dismutase, catalase, glutathione reductase and glutathione-S-transferase enzymes was noticed in the infected groups but this decrease in activities was less in WSR and AVL extract supplemented group indicating antioxidant property of these extracts. WSR extract supplemented group revealed significantly higher E. coli specific antibody titer and enhanced lymphocyte proliferation response as compared to control. AVL extract supplemented group also revealed increase in humoral and cell mediated immune response, though non-significant. The gross and histopathological lesions in E. coli infected chicks were fibrinous pericarditis and perihepatitis, myocarditis, hepatitis, congestion in visceral organs, peritonitis, bronchopneumonia, enteritis and necrosis and depletion of lymphocytes in spleen and bursa of Fabricius. WSR extract supplementation produced 31.48% and 34.38% protection in these gross and histopathological lesions, respectively. AVL extract supplementation produced protection of 14.19% and 17.5% in gross and histopathological lesions. It is concluded that supplementation of 20% WSR extract @ 20ml/liter of water caused reduction in severity, mortality and recovery period of E. coli infection and enhanced the humoral and cellular immune responses suggesting its protective effect on limiting the pathology and pathogenesis of E. coli infection in broiler chickens. AVL extract possessed good in vitro antibacterial effect against E. coli but in vivo study in broiler chicken did not show considerable ameliorating effect on pathological changes of E. coli infection.