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Chaudhary Sarwan Kumar Himachal Pradesh Agriculture University, Palampur

Himachal Pradesh Krishi Vishvavidyalaya (renamed as Chaudhary Sarwan Kumar Himachal Pradesh Krishi Vishvavidyalaya in June, 2001) was established on 1st November, 1978.The College of Agriculture (established in May, 1966) formed the nucleus of the new farm University. It is ICAR accredited and ISO 9001:2015 certified institution. The Indian Council of Agricultural Research has ranked this University at eleventh place among all farm universities of the country. The University has been given the mandate for making provision for imparting education in agriculture and other allied branches of learning, furthering the advancement of learning and prosecution of research and undertaking extension of such sciences, especially to the rural people of Himachal Pradesh. Over the years, this University has contributed significantly in transforming the farm scenario of Himachal Pradesh. It has developed human resources, varieties and technologies and transferred these to farming community enabling the State to receive the “Krishikarman award” of Govt. of India four times in row for food grain production among small states of the country. Today, the State has earned its name for hill agricultural diversification and the farming community has imposed its faith in the University.

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Subject: Agricultural Biotechnology × End date: 2018 × Start date: 2018 ×
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    ThesisItemOpen Access
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    ThesisItemOpen Access
    MARKER ASSISTED AND DOUBLED HAPLOIDY BREEDING FOR THE DEVELOPMENT OF BLAST AND BACTERIAL BLIGHT RESISTANT RICE PYRAMID LINES
    (CSKHPKV, Palampur, 2018-07-24) Chauhan, Ruchi; Kapila, R.K.
    Rice (Oryza sativa L.) serves as a major carbohydrate source for nearly half of the world’s population and provides livelihood to the people of Asia. Rice blast caused by Magnaporthe grisea and bacterial leaf blight caused by Xanthomonas oryzae pv.oryzaeare the two major diseases affecting rice productivity in north-western Himalayan region of India. Biotechnological tools like anther culture and molecular marker technology hold a great role as a catalyst in accelerating the pace of blast and bacterial blight resistance breeding. In order to pyramid 2 blast (Pi9 and Pita) and 2 bacterial blight (Xa21 and Xa38) resistance genes in a popular rice variety ‘HPR2143’, foreground selection was done to identify single gene positive BC2F2 homozygous progenies of 4 crosses, viz. HPR2143/PB1 (Pi9), HPR2143/DHMAS164 (Pita), HPR2143/IRBB54 (Xa21) and HPR 2143/PR114 (Xa38) using gene derived markers. The foreground selection resulted in identification of 5 plants homozygous for the gene Pita, 20 for Pi9, 12 for Xa21 and 6 for Xa38.In order to pyramid two genes each against rice blast, progenies positive for gene Pita were crossed reciprocally with progenies positive for the gene, Pi9. Likewise, the homozygous positive plants for genes, Xa21 and Xa38 were crossed reciprocally to produce F1s. Overall crossability among the selected derivatives over both cross combinations (Pita × Pi9) and (Xa21 × Xa38) was recorded to be 38.61%. Anther culture of developed F1s was attempted for fixation of genes. For cross (Pita + Pi9 and reciprocals), overall callus induction and regeneration frequency of 9.2% and 6.5%, respectively was recorded. The frequency of green plantlet regeneration recorded was 29.9% thereby resulting in an overall anther culture efficiency of 0.18% from a total of 22562 anthers cultured. For cross 2 (Xa21× Xa38 and reciprocals), observed callus induction and regeneration frequency was 9.08% and 5.7%, respectively. A total of 88 regenerating calli resulted in regeneration of 20 green (22.7%) and 68 albino plants (77.2%) with an overall anther culture efficiency of 0.11% in a total of 16894 cultured anthers. Out of 57 plants, 28 were positive for the gene combination, Pita + Pi9 and 15 for Xa21 + Xa28. Field evaluation of 38 generated doubled haploid pyramid (DHP) lines having sufficient seeds revealed presence of significant variation among the lines for all nine traits studied. Based on their performance, promising DHP lines for different economic traits were identified including three best DHP lines, i.e. DHP57, DHP4 and DHP16 performing close to the check, HPR2143 for seed yield/plant. The material generated can be used directly for identification of variety(ies) as well as can further be used as genetic stocks in the future breeding programmes for developing broad spectrum and durable multiple disease resistance against rice blast and bacterial leaf blight diseases
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    ThesisItemOpen Access
    MARKER ASSISTED SELECTION FOR BEAN ANTHRACNOSE RESISTANCE IN BACKCROSS DERIVATIVES OF FRENCH BEAN
    (CSKHPKV, Palampur, 2018-07-24) Manohar, Jadhav Harshad; Kapila, R.K.
    French bean (Phaseolus vulgaris L.) is one of the most important food legumes consumed worldwide including India. Viable production of French bean in the temperate and sub-temperate regions of the north western Himalayas is hampered by anthracnose disease caused by fungus Collectrotrichum lindemuthianum. The disease is favoured by cool and humid environment that is prevalent in many parts of Himachal Pradesh. The present study was undertaken on marker assisted backcrossing of bean anthracnose resistant Co-4 gene positive F5 progenies of a cross, Arka Komal × TO to elite parent, Arka Komal. Three previously selected gene positive derivatives of French bean possessing desirable characters like better pod length (11-9-1), higher branch number (11-9-2) and early flowering (11-2-2) were used as donors of anthracnose resistance gene, Co-4. These were backcrossed with elite but susceptible parent, Arka Komal to generate BC1F1 pods. The crossability of elite derivatives with recurrent parent ranged from 1.92% to 7.14% under the screen house conditions. The highest crossability was observed between derivative 11-2-2 and the recurrent parent. Testing of hybridity of the resultant BC1F1 plants using gene linked SCAR marker SY20 resulted in identification of 5 true BC1F1 pods. The resulting BC1F1 raised were allowed to self-pollinate to produce BC1F2 seeds. In the next season, 20 BC1F2 seeds of cross Arka Komal × 11-9-1, 40 of cross combination Arka Komal × 11-9-2, and 18 of combination Arka Komal × 11-2-2 which were healthy and well filled, were raised in the screen house. The results of the foreground selection in these plants revealed that in cross combination Arka Komal × 11-9-1, out of 20 plants screened for the presence of SY20 marker, 15 were found to be positive for the marker and hence the gene, Co-4. Similarly, for cross combination Arka Komal × 11-9-2, the successful amplification was noticed in 30 out of a total of 40 plants. In combination Arka Komal × 11-2-2, out of a total of 18 plants, 8 amplified the desired product for marker, SY20. Thus, overall, out of the 78 plants raised, 53 were inferred to be Co-4 gene positive. A further validation to confirm the introgression of the Co-4 gene was done by Detached Pod Method and the results established cent per cent accuracy and efficiency of the marker SY20 linked to the bean anthracnose resistance gene, Co-4. Keywords: French bean, Anthracnose, Collectrotrichum lindemuthianum, Co-4, Arka Komal, SY20.