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Chaudhary Charan Singh Haryana Agricultural University, Hisar

Chaudhary Charan Singh Haryana Agricultural University popularly known as HAU, is one of Asia's biggest agricultural universities, located at Hisar in the Indian state of Haryana. It is named after India's seventh Prime Minister, Chaudhary Charan Singh. It is a leader in agricultural research in India and contributed significantly to Green Revolution and White Revolution in India in the 1960s and 70s. It has a very large campus and has several research centres throughout the state. It won the Indian Council of Agricultural Research's Award for the Best Institute in 1997. HAU was initially a campus of Punjab Agricultural University, Ludhiana. After the formation of Haryana in 1966, it became an autonomous institution on February 2, 1970 through a Presidential Ordinance, later ratified as Haryana and Punjab Agricultural Universities Act, 1970, passed by the Lok Sabha on March 29, 1970. A. L. Fletcher, the first Vice-Chancellor of the university, was instrumental in its initial growth.

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    OtherItemOpen Access
    Technical Programme 2014-15
    (CCS HAU, Hisar, 2014) Agricultural Economics; College of Agriculture
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    ThesisItemOpen Access
    Effect of cold storage on some biological characteristics of parasitoid, Aenasius bambawalei Hayat (Hymenoptera: Encyrtidae)
    (CCSHAU, 2014) Rathee, Mandeep; Pala Ram
    The present studies on cold storage and overwintering of Aenasius bamabawalei were carried out during 2012-13 both in the laboratory as well as in the screen house of the Department of Entomology, CCS Haryana Agricultural University, Hisar to generate information on effect of cold storage of the parasitoid, A. bambawalei in different stages for different durations at different temperatures on its biological parameters and to evaluate the survival of the parasitoid during winter season. Studies on cold storage of the parasitoid were carried out by storing one-day-old Phenacoccus solenopsis mummies (i.e. in pupal stage) and freshly emerged adults at four storage temperatures i.e. 5, 10, 15 and 20°C for 1 to 8 weeks. It was found that the parasitoid A. bambawalei can be stored in pupal stage (i.e. in the mummies of P. solenopsis) for 1 week at 5°C and for 2 weeks each at 10 and 15°C, respectively, without any significant effect on the key biological parameters like emergence, longevity, fecundity and sex-ratio (F1 progeny). Emergence of the adults occurred within first week during storage at 20°C, hence, this temperature was not suitable for storing the parasitoid in pupal stage. Females took more time to emerge from the mummies stored for different durations at 5, 10 and 15°C as compared to males. The parasitoid adults of A. bambawalei can be stored only for 1 week at 10°C without any significant affect on the key biological parameters like survival, longevity, fecundity and sex-ratio (F1 progeny). The parasitoid A. bambawalei can be stored at low temperatures both in pupal and adult stages. However, it can be stored safely for longer duration in pupal stage as compared to adult stage. Emergence of parasitoid adults from overwintering mummies started from 11th standard week (first week of February), being maximum in 14th standard week (first week of March) and continued until 16th standard week (third week of March) when all the parasitoid adults had emerged. Maximum temperature ranged between 20.3 to 29.6oC and minimum between 6.6 to 12.5°C during the period of adult emergence. Honey fed parasitoid adults i.e. males survived for 52-84 days (69.33 days) and females survived for 69-103 days (83.40 days) during winter season in absence of host, when exposed on 5th December. The results of the present studies may be helpful in mass rearing and conservation of the parasitoid.
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    ThesisItemOpen Access
    Diversity, abundance and pollination efficiency of insect pollinators on seed crop of broccoli (Brassica oleracea L. var. italica Plenck)
    (CCSHAU, 2014) Suman Devi; Ombir
    The present study was conducted at Chaudhary Charan Singh Haryana Agricultural University, Hisar, Haryana (India). Twenty seven insect species belonging to 14 families under 5 orders were observed visiting the flowers of broccoli. In variety BFT-1, A. dorsata was the most abundant visitor (2.90) followed by A. mellifera (2.30), A. cerana (1.20), A. florea (0.80) and syrphid fly were least frequent visitor (0.20), in variety GH-1 A. mellifera was the most abundant visitor (7.34) followed by A. florea (6.67), A. dorsata (2.15), syrphid fly (1.84) and A. cerana (1.22) and in varieties LPH-1 and DPH-1, A. mellifera was the most abundant visitor (5.96 and 7.00) followed by A. florea (4.46 and 5.50), syrphid fly (2.97 and 3.30), A. dorsata (2.62 and 2.41 ) and A. cerana (1.66 and 2.40). The mean foraging speed, in varieties BFT-1, GH-1, LPH-1 and DPH-1 was maximum in case of A. florea (28.29, 38.54, 37.44 and 36.72, respectively) followed by A. dorsata (5.19, 5.24, 5.24 and 5.20, respectively), A. mellifera (4.54, 4.54, 4.52 and 4.50, respectively ), A. cerana (3.56, 3.51, 3.58 and 3.59, respectively) and syrphid fly (1.90, 2.65, 2.67 and 2.60, respectively). The mean foraging rate in varieties BFT-1, GH-1, LPH-1 and DPH-1 was maximum in case of syrphid fly (14.18, 17.61, 17.42 and 17.33, respectively) followed by A. cerana (10.26, 10.29, 10.27 and 10.32, respectively), A. mellifera (9.27, 10.04, 10.00 and 10.31, respectively), A. dorsata (7.84, 7.88, 7.77 and 7.80, respectively) and A. florea (1.42, 1.71, 1.71 and 1.72, respectively). The average number of broccoli siliqua/plant, siliqua length, number of seeds/siliqua, seed vigour-I, seed vigour-II, test weight and germination per cent under open-pollination were significantly higher than without insect pollination. Number of siliqua/plant, number of seeds/10 siliqua, siliqua length (cm), test weight (g), germination (%) and seed vigour-I, seed vigour-II of uncaged and caged broccoli plants were 3,362.0 and 672.6, 118.75 and 25.85, 6.79 and 3.54, 2.888 and 2.092, 87.50 and 30.25, 1,471.65 and 418.40 and 0.662 and 0.112, respectively.
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    ThesisItemOpen Access
    Biodiesel production from lipid generating microalgae
    (CCSHAU, 2014) Narula, Amrita; Anand, R.C.
    In the present scenario, the future of crude oil looks quite bleak as it may lead to all oil resources coming close to a moribund one day. Moreover, consumption of present petroleum sourced fuels at the rocket speed rate has also led to various environmental problems. One of the major concern is global warming. To make a dent in global warming, bioenergy must be generated at a very high speed. Microalgae may be the best option to produce bio-energy at rates high enough to replace a substantial fraction of fossil fuel used by our society. The primary objective of this study was to isolate lipid generating microalgal isolates for biodiesel production. The microalgal water samples were collected from ponds of different locations of eight districts of Haryana including Hisar, Rohtak, Fatehabad, Sirsa, Panipat, Karnal, Ambala and Kurukshetra. The samples were analysed for pH, EC, salinity, turbidity, total nitrogen, phosphorous and potassium. The microalgal samples were enriched in the Bold’s Basal medium and incubated at 23 ±10C, 50-55% humidity with 16:8 hours light: dark photoperiod for 21 days. A total of twenty four microalgal isolates were obtained after purification. Using Nile red staining technique microalgal isolates HMA-2 and FMA-2 were selected for further study and grown in four media of different chemical composition viz., Soil extract, Chu-13, Kuhl and Bold’s Basal medium. The microalgal isolates HMA-2 and FMA-2 produced maximum biomass (0.21 and 0.29g/l ) in Bold’s Basal medium whereas lipid content (29.6 and 25.2%) was maximum in Kuhl medium when grown at 23 ±10C, 50-55% humidity with 16:8 hours light: dark photoperiod for 21 days. Among the inorganic nitrogen sources tested potassium nitrate (original source) resulted in biomass accumulation of 0.12 and 0.19 g/l with 29.4 and 25.2% lipid content in the microalgal isolates HMA-2 and FMA-2 respectively while among organic nitrogen sources, peptone supplementation showed maximum lipid content of 38.2 and 22.1% from 0.04 g/l and 0.10 g/l biomass respectively. Similarly, among the carbon sources, glucose had been found to be a better supplement as it produced biomass of 1.59 and 1.21 g/l with 57.6 and 50.2% lipid content in microalgal isolates HMA-2 and FMA-2 respectively. Lipid and biomass production studies at different pH, temperature and salinity indicated biomass production of 0.14 g/l having 32.6% lipid at pH 8, 0.23g/l biomass having 42.4% lipid at 250C and 0.07g/l biomass containing 61.4% lipid on 20 g/l sodium chloride supplementation in microalgal isolate HMA-2 whereas in isolate FMA-2, 0.13, 0.21 and 0.11g/l biomass containing 26.1, 40.2 and 36.2% lipid was obtained under similar conditions respectively. Transesterification of algal oil of microalgal isolate HMA-2 using NaOH as catalyst and methanol resulted in biodiesel production to the tune of 9%.
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    ThesisItemOpen Access
    Technological gap and training needs of Bt-cotton growers in Haryana
    (CCSHAU, 2014) Davender Kumar; Hudda, R.S.
    The present study was conducted in the native state Haryana. Out of 21 disticts, in the state, two districts namely Sirsa and Hisar has largest area and production under Bt-cotton among all the district of Haryana state and contributes about 50 per cent of Bt cotton production in the state. Cotton is soft, staple fiber that grows around the seeds of the cotton plants (Gossypium sp.), a shrub native to tropical and subtropical regions around the world, including the America, India and Africa. All the commercial cotton is Native American species (Gossypium hirsutum and Gossypium barbadense). The overall technology gap was found to be about 47.50 per cent. Aspect wise of the respondents fall in high level of technology gap. It was high in case of varieties and agronomic practices (52.08 per cent and 45.42 per cent respectively). It was observed from the data that 49.17 high level of sowing methods of the respondents were found to be having high level of manures and fertilizer application 50.83 per cent. It was very much clear from the table that 54.58 per cent of the respondents had high level of technology gap in pest and disease management.as for as picking of cotton was concerned, the most of the respondents 41.25 farmers belong to high technology gap When asked about their interest and needs for various training programme, almost all the farmers showed their interest in obtaining the training for the Bt-cotton growers farmers in different subject matter areas Insects scoutin, (88.75%) Application and use of chemicals (86.66%) Features of Bt-cotton technology (83.75%), Bt-cotton varieties/hybrid (82.50%).
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    ThesisItemOpen Access
    Molecular breeding for developing grain protein and micronutrient (Iron and Zinc) dense Indian bread wheat genotypes
    (CCSHAU, 2014) Tomar, Luxmi; Yadav, Neelam R.
    Marker assisted selection strategy was used to introgress the functional Gpc-B1 allele in Indian wheat varieties (WH711 and PBW343) from Lassik, a hard red spring wheat. Fifty two of the most widely accepted Indian varieties were screened for their GPC and micronutrient (Zinc and Iron) content. A narrow range of variability was observed in GPC (9.3-13.7%), zinc (25.8-54.1mg/kg) and iron (37.5-57.9mg/kg) content. H711 and PBW343 were selected and crossed with Lassik. F2 plants were screened for presence/ absence of functional Gpc-B1 and rust resistance genes Lr37/Yr17/Sr38 and Lr34/Yr18 using allele specific amplification (ASA) markers. Plants from F2 observed, on an average 26.4% increase in GPC, 37.5% in zinc and 64.8 % in iron content. The plants did not show any rust susceptibility under field conditions. F2 plants with minimum reduction in yield were chosen for back-crossing with their respective Indian parent. Plants obtained from subsequent back crosses i.e . BC1F1 and BC1F2, also had better quality attributes than the parents. The GPC increased on an average by 30.2% and twice zinc (90.2%) and iron (52.5%) as compared to the recurrent parent. Hybrid plants also had better agronomical properties and showed resistance to stripe rust under field conditions whereas parental genotypes faced significant yield loses. These plants showed complete rust resistance due to presence of Gpc-B1/Yr36 with the Lr37/Yr17/Sr38 and Lr34/Yr18 loci. This population has all the combined benefits of better nutritional quality, yield and disease resistance and could be used both in MAS as well as for transferring the Gpc-B1in other Indian varieties to minimize the linkage drag. Though introgression of Gpc-B1 was linked with a net reduction in yield, interestingly, some of these plants with functional Gpc-B1 allele had higher TKW than either of the parents emphasizing the extent of influence of GxE interaction could have on Gpc-B1 allele. A consensus between quality and quantity has to be reached while breeding for improving GPC in wheat.
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    ThesisItemOpen Access
    Risk Perception and Capacity Building of Women in Diversified Agriculture for Entrepreneurship
    (CCSHAU, 2014) Ritu; Yadav, Lali
    The study on, “Risk perception and capacity building of women in diversified agriculture for entrepreneurship” was conducted covering 200 randomly drawn respondents from four villages of purposively selected Hisar district of Haryana. Most respondents played active participation, showed information seeking behavior and consulting role in decision making for diversified agricultural activities. Most respondents perceived training needs for dairy at commercial level, taking measures for clean milk production, vermicomposting, food processing and purchase and sale of milch animals. Majority respondents perceived risk in terms of uncertain whether condition, inadequate knowledge and skill of innovative appropriate irrigation technology, rising price of agricultural inputs, problem of middleman and high and varying interest rate. Most respondents were willingly to undertake diversified entrepreneurial agricultural activities, were highly willing to change the existing pattern of farming diversification. Huge majority respondents were willing to pay to undertake specified training with rider to undertake diversified agricultural activities as an entrepreneurial. The perceived entrepreneurial attributes to undertake diversified agricultural activities were creativity, innovativeness, achievement motivation and rationality for dairy, ability to motivate others, self-confidence, skill competence and foresightedness for floriculture, creativity, environmental scanning, change proneness and goal setting skills for vermicomposting, communicability, environmental scanning, skill competence and goal setting skill for food processing, creativity, self-confidence, change proneness and rationality in thinking for medicinal and aromatic plants. Majority scored high on perceived attributes for all the technologies (serrated sickle, coat bag and capron) except sag cutter. Significant knowledge gain on all activities was observed. Significant association was observed for independent and dependent variables.
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    ThesisItemOpen Access
    Linkage mapping of quantitative trait loci for root morphology and selection of desirable genotypes from aerobic x lowland indica crosses in rice (Oryza sativa L.)
    (CCSHAU, 2014) Kharb, Anju; Jain, Rajinder Kumar
    Identification of stable QTL for traits promoting aerobic adaptation using molecular markers can greatly enhance the efficacy of breeding programs to develop water-efficient, high yielding, aerobic rice varieties. Filial (F2 and F3) and backcross ( BC2F2 and BC1F2) populations derived from the crosses between high-yielding low-land (HKR47) and aerobic (MAS26 and MASARB25) indica rice varieties, displayed large variation for various physio-morphological traits including grain yield per plant and root traits (in case of net house evaluation). Phenotypic correlation analysis showed positive correlation (r = 0.279 and 0.351) between grain yield per plant and root length in both F2 and F3 population and between grain yield per plant and fresh & dry root weight (r = 0.232 and 0.269) in F2 population. Under aerobic field conditions, Grain yield per plant showed significant positive correlation with plant height, effective number of tillers/plant and panicle length in all the four populations. A total of 803 SSR markers, distributed on 12 rice chromosomes, were analyzed for polymorphism in two parental rice genotypes (HKR 47 and MAS 26); of these 125 (about 16%) displayed polymorphism. SSR fingerprint databases of three HKR47 x MAS26 F2 and F3 populations (94 F2 in net house, 42 F3 in net house and 52 F3 plants in field) were prepared using 125, 88 and 88 polymorphic SSR markers, respectively. NTSYS-pc UPGMA tree cluster analysis and two-dimensional PCA scaling showed scattering of the F2 and F3 populations between the two distinct parental genotypes; the populations were inclined towards MAS26. Composite interval mapping (CIM) analysis by Win QTL cartographer 2.5 revealed a total of six QTLs (qPN8.1, qPN8.2, qPN8.3, qTGW8.1, qYPP8.1 and qRL8.1) in HKR47 x MAS26 F2 population, grown in the net house on chromosome 8 (within a region of 24.9 cM) which individually explained 13.7-27.3% of the phenotypic variation. In case of HKR47 x MAS26 F3 population, based on field data and net house data, two (qTGW6.1 and qLB8.1) and six QTL (qPH3.1, qLB8.1, qTGW6.1, qTGW11.1, qRT8.1 and qRT8.2) were mapped, respectively. As many as 20 F3, 6 BC2F2 and 5 BC1F2 promising plants having higher grain yield and/or better root length/biomass were selected. Data on monitoring of putative QTL identified showed that out of twenty selected plants, four plants had 13 QTL, three plants had 12 QTL and two plants had 11 QTL in homozygous or heterozygous condition.
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    ThesisItemOpen Access
    Evaluation of seed vigour parameters for heat tolerance in bread wheat
    (CCSHAU, 2014) Arun Kumar; Dahiya, O.S.
    Wheat (Triticum aestivum L.) is a widely adapted crop. It is grown from temperate, irrigated to dry and high-rain-fall areas and from warm, humid to dry, cold environments. The genetic purity, physical purity, viability, vigour and uniform seed size are the most important parameters to determine the quality of seed. High seed germination and vigour are pre- requisites for the success of stand establishment of crop plants. Generally stress (moisture/heat) has deleterious effect on germination and vigour of crop.The present investigation was carried out at the department of Seed Science and Technology, CCS Haryana Agricultural University, Hisar. The material comprised of six each heat tolerant and susceptible varieties which were sown on two date i.e. normal (19th, 20th November) and late sown (19th, 20th December). The observations were recorded at grain filling stage (heading to maturity). Heat tolerant varieties had performed better at different stages of seed development in comparison to susceptible. Heat tolerant varieties were found significantly higher for seed weight, seed moisture content, germination, seedling length, vigour index-I & II, seedling dry weight and than susceptible varieties under normal and late sowing condition in both the year. Heat tolerant varieties were recorded significantly lower for days to heading, physiological and harvestable maturity. It means heat tolerant varieties mature earlier than susceptible. Commonly varieties had performed better for all characters studied in normal than late sowing at all stages of seed development. Heat tolerant varieties recorded higher for membrane Thermostability and lower for chlorophyll fluorescence. This might be effect of heat stress during seed development. Both vigour index-I and II were significantly and negatively correlated with maximum, minimum and difference of temperature but positively with sunshine hour and relative humidity in both the years of data recording. It means that as optimum temperature for cultivation of wheat is increasing then vigour of the seed will be decreases. For normal as well as late sown heat tolerant varieties having more DHA, peroxidase, catalase and superoxide dismutase activity at all stages than heat susceptible. All stress enzyme activity was higher in late than normal sowing which was in concurrence with high temperature during seed development. Number of grains per spike (average of five plants), number of grains per spike and grain yield per plant was higher for normal sown than late sown in both the year. Varieties have performed better in second year than in first year. Heat tolerant has performed better than susceptible for almost all agronomic traits. Based on both the tests it can infer that there was better relative storability of the variety for normal than late sowing over the years at all stages of storage.