Thumbnail Image

Chaudhary Charan Singh Haryana Agricultural University, Hisar

Chaudhary Charan Singh Haryana Agricultural University popularly known as HAU, is one of Asia's biggest agricultural universities, located at Hisar in the Indian state of Haryana. It is named after India's seventh Prime Minister, Chaudhary Charan Singh. It is a leader in agricultural research in India and contributed significantly to Green Revolution and White Revolution in India in the 1960s and 70s. It has a very large campus and has several research centres throughout the state. It won the Indian Council of Agricultural Research's Award for the Best Institute in 1997. HAU was initially a campus of Punjab Agricultural University, Ludhiana. After the formation of Haryana in 1966, it became an autonomous institution on February 2, 1970 through a Presidential Ordinance, later ratified as Haryana and Punjab Agricultural Universities Act, 1970, passed by the Lok Sabha on March 29, 1970. A. L. Fletcher, the first Vice-Chancellor of the university, was instrumental in its initial growth.

Browse

2000 - 20062
Reset filters
Author: Verma, Raj Kumar × Start date: 2000 × Type: Thesis ×
Reset filters

Search Results

Now showing 1 - 2 of 2
  • Thumbnail Image
    ThesisItemOpen Access
    Studies On Formulation Of Infectious Bursal Disease Virus Immune Complex Vaccine And Its Effect On The Sequential Pathology Of The Disease In Broiler Chicks
    (Chaudhary Charan Singh Haryana Agricultural University; Hisar, 2000) Verma, Raj Kumar; Verma, P.C.
  • Thumbnail Image
    ThesisItemOpen Access
    In vitro studies in acid lime (Citrus aurantifolia Swingle) cv. kagzi lime
    (CCSHAU, 2006) Verma, Raj Kumar; Sultan Singh
    The experiment was conducted during 2004-2006 on in vitro studies in acid lime (Citrus aurantifolia Swingle) var. Kagzi Lime in the laboratory of Centre for Research and Application in Plant Tissue Culture CRATPC CCS HAU, Hisar Haryana. The experiment consisted of in vitro raising of seedlings and direct shoot regeneration in acid lime var. Kagzi Lime. The experimental material comprised of seeds and different explants (in vitro shoot tip and nodal segment, in vivo shoot tip and nodal segment) with different combinations of growth regulators (GA3, BAP, NAA, IBA) supplementing the MS basal medium. For axenic production of seedling through in vitro seed germination, the best medium was MS medium + GA3 0.5 mg/l and for seedling growth best medium was MS medium + GA3 0.5 + BAP 0.5 mg/l. Direct regeneration of shoots, roots and whole plant without intervention of callus from both in vitro and in vivo shoot tip and nodal segment on MS basal medium containing BAP along with NAA at different concentrations was observed. The MS medium + BAP 0.25 mg/l wa observed to be the best medium for shoot regeneration inducing 90 percent in both in vitro shoot tip and nodal segment and 77.77 and 78.59 percent in in vivo shoot tip and nodal segment respectively. The half MS medium + IBA 1.0 mg/l was found to be best for different aspects of root differentiation like number of days required for root initiation (13.80) and completion of rooting (25.60), percent rooting (100%), number of roots/plantlet (3.80), root length (3.88cm) and percent survival of rooted plantlets (100%). The maximum survival percentage of micro propagated plantlets under green house condition and open condition after hardening period of four weeks was 100 percent in the potting mixture having soil, sand and FYM in an equal proportion (1:1:1 v/v). Improved transformation frequencies were obtained by co-cultivation the explants with Agrobacterium on feeder plants by using kanamycin at 100 mg/l as selective agent. Attempts to use geneticin as selection antibiotic were not successful. The presence and expression of transferred genes was verified by -glucuronidase histochemical expressed as blue spots in co-cultivated explants.