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Chaudhary Charan Singh Haryana Agricultural University, Hisar

Chaudhary Charan Singh Haryana Agricultural University popularly known as HAU, is one of Asia's biggest agricultural universities, located at Hisar in the Indian state of Haryana. It is named after India's seventh Prime Minister, Chaudhary Charan Singh. It is a leader in agricultural research in India and contributed significantly to Green Revolution and White Revolution in India in the 1960s and 70s. It has a very large campus and has several research centres throughout the state. It won the Indian Council of Agricultural Research's Award for the Best Institute in 1997. HAU was initially a campus of Punjab Agricultural University, Ludhiana. After the formation of Haryana in 1966, it became an autonomous institution on February 2, 1970 through a Presidential Ordinance, later ratified as Haryana and Punjab Agricultural Universities Act, 1970, passed by the Lok Sabha on March 29, 1970. A. L. Fletcher, the first Vice-Chancellor of the university, was instrumental in its initial growth.

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Author: Pardeep Kumar × End date: 2009 × Type: Thesis ×
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    ThesisItemOpen Access
    Under Graduate Curriculum In Agriculture - A Prespective Study
    (Chaudhary Charan Singh Haryana Agricultural University;Hisar, 2000) Pardeep Kumar; Dalal, R S
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    ThesisItemOpen Access
    Studies on the management of black scurf of potato caused by rhizoctonia solani kuhn.
    (CCSHAU, 2005) Pardeep Kumar; Gandhi, S.K.
    The fungus Rhizoctonia solani Kuhn causing black scurf of potato was isolated and purified. Among seven bioagents evaluated in vitro against R. solani by dual culture method fungal bioagents viz., Trichoderma viride, T. harzianum and G. virens showed their maximum potential at lower pH (5 and 6) while bacterial bioagents viz., Pseudomonas fluorescens, P. maltophila, Bacillus subtilis and Azotobacter chroococcum were more active in the alkaline range. However, T. viride was effective in wide pH range. Similarly when bioagents were tested at different temperature regimes P. maltophila and T. viride showed their potential over a wide temperature range while others were effective in the temperature range of 20-30 oC. On the basis of antagonistic potential of bioagents in vitro five bioagents were selected for evaluation in the field both as seed and soil treatment. Best results in terms of reduction of disease severity were obtained in case of P. maltophila and T. viride. Further both the antagonists increased the tuber yield appreciably in comparison to check. Plant height was not affected by these treatments. Tubertreatment done with few resistance inducing chemicals viz., indole acetic acid, indole butyric acid, salicylic acid, nicotinic acid and benzoic acid at 0.125 and 0.25 per cent concentrations revealed salicylic acid to be most effective in reducing the disease followed by nicotinic acid. At 0.125 per cent concentration reduction in plant height was noticed on IAA and IBA at 30 days after sowing which recovered at 60 days after sowing. Effect of these chemicals on tuber yield were not well marked.
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    ThesisItemOpen Access
    Preparation and storability of garlic powder
    (CCSHAU, 2009) Pardeep Kumar; Garg, M.K.
    The present investigation entitled “Preparation and storability of garlic powder” was carried out with the objectives to study the effect of slicing and flaking of garlic on the drying kinetics and quality. The effect of PEF (Pulsed Electric Field) treatment and the packaging material on the storability of garlic powder were also studied. The fresh garlics were analyzed for proximate analysis. Garlics were then dehydrated and analyzed for drying rate, dehydration ratio, rehydration ratio. Then dehydrated garlics were ground into fine powder and analyzed for bulk density, hunterlab (L*a*b*) values, total sugars and the organoleptic quality. It was found that fresh peeled garlics contained moisture-63.78 percent, protein-5.99 percent, ash-1.91 percent, fat-1.75 percent, crude fiber-1.13 percent and carbohydrates-25.44. Garlic slices and flakes were prepared and different treatment viz., control (without PEF treatment) and PEF (9000V) were given to the samples of garlic. After the pre-treatment, the samples (slices and flakes) were dried in a tray drier at 600C temperature. Then dehydrated garlic were ground into fine powder and packed into LDPE (low density polythene), aluminium foil laminate and glass bottle (amber colour) for storage and were analysed for total sugars, bulk density and organoleptic quality to evaluate the product quality. PEF (9000V) was done for inactivation of micro-organism and enzymes which cause spoilage in the product and increase the shelf-life of the product. PEF treatment resulted in the increase of drying rate and faster removal of the moisture. Three packaging materials were used to pack the garlic powder. There was no significant effect of packaging materials and pre-treatment on the bulk density of garlic powder. L* (lightness) value was good for PEF treated samples and for samples which were packed in aluminium foil laminate and glass bottle (amber colour). The organoleptic score for garlic powder was found good for PEF treated samples and for samples packed in aluminium foil laminate and glass bottle (amber colour).
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    ThesisItemOpen Access
    Expression of myb gene in brassica tournefortii L.under drought stress
    (CCSHAU, 2009) Pardeep Kumar; Yadav, Neelam R.
    Brassica tournefortii, a highly drought tolerant Brassica species was used to study myb gene expression under drought stress. Seeds of Brassica tournefortii were grown on MS medium. Then 14 days seedlings were uprooted and given drought stress by subjecting them to air drying, PEG (-2 to -8 bar) and mannitol (100 mM to 400 mM) treatments. Total RNA isolation from stressed seedlings was carried out using Trizol reagent yielding 17.52-36.8 Fg/ml of total RNA. One- step RT-PCR was carried out using total RNA as template with BjMyb-1 primer designed from conserved domain of AtMyb2 and its homologous. BjActin primers were used in RT-PCR which served as control, as actin gene is constitutively expressed in all tissues. Exposure to drought stress for 15 minutes and 30 minutes (air drying) gave no amplification showing air drying upto 30 minutes does not induce any myb expression. An amplified product of 250 bp was obtained on exposure to drought stress for 60 minutes with air drying, PEG (-2 to -8 bar) and mannitol (100 mM to 400 mM) treatments. The transcript level was found similar in all treatments irrespective of drought treatments. cDNA was eluted out from the gel and purified cDNA was transformed using pDrive cloning vector (Quigen) in XL-blue strain of E.coli using blue- white selection. Transformed clones were characterized by plasmid DNA isolation, PCR amplification of plasmid DNA with gene specific primers. Plasmid DNA from transformed clones showed higher molecular weight than untransformed plasmid DNA on agarose gel electrophoresis confirmed the insertion of DNA fragment into the plasmid. PCR amplification of plasmid DNA also confirmed the successful cloning.