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Chaudhary Charan Singh Haryana Agricultural University, Hisar

Chaudhary Charan Singh Haryana Agricultural University popularly known as HAU, is one of Asia's biggest agricultural universities, located at Hisar in the Indian state of Haryana. It is named after India's seventh Prime Minister, Chaudhary Charan Singh. It is a leader in agricultural research in India and contributed significantly to Green Revolution and White Revolution in India in the 1960s and 70s. It has a very large campus and has several research centres throughout the state. It won the Indian Council of Agricultural Research's Award for the Best Institute in 1997. HAU was initially a campus of Punjab Agricultural University, Ludhiana. After the formation of Haryana in 1966, it became an autonomous institution on February 2, 1970 through a Presidential Ordinance, later ratified as Haryana and Punjab Agricultural Universities Act, 1970, passed by the Lok Sabha on March 29, 1970. A. L. Fletcher, the first Vice-Chancellor of the university, was instrumental in its initial growth.

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Subject: Plant Physiology × Author: Chand, Gurdev × End date: 2015 × Start date: 2010 × Type: Thesis ×
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    ThesisItemOpen Access
    Physiological and reproductive behaviour of summer Mungbean [Vigna radiata (L.) Wilczek] under terminal heat stress
    (CCSHAU, 2015) Chand, Gurdev; Nandwal, A.S.
    The present investigation was carried out on three mungbean genotypes viz. MH 421, MH 318 and Basanti in the screen house during 2012-13 and 2013-14. High temperature induced changes for plant water status i.e. water potential (yw), osmotic potential (ys) and relative water content (RWC %), relative stress injury (RSI %), physiological parameters viz. total chlorophyll, chlorophyll stability index (CSI), chlorophyll fluorescence and canopy temperature depression (CTD), biochemical like malondialdehyde (MDA), total soluble carbohydrate (TSCs), proline accumulation, nitrogen content, antioxidant enzymes and protein profile were studied 3 and 7days after exposure (DAE) to high temperature (>35oC) and reproductive changes, yield attributing character, seed quality studies were taken at harvesting stage. The yw and ys of leaves became more negative from -0.66 to -1.97 and -1.01 to -1.87 MPa respectively, with increasing the days after exposure (DAE) to high temperature (>35°C). In the genotype MH 421, the value of yw (-1.59) and ys (-1.69) of leaf were more negative than MH 318 and Basanti. With the increased DAE, RWC of leaf declined from 73.65% to 63.41%. Total chlorophyll content (17.27 to 11.31 mg g-1 DW), SPAD (48.64 to 46.02) readings, CTD (-5.78 to -4.37: °-C) and CSI (81.01% to 67.36%) decreased with increased DAE to high temperature and decrease was more in Basanti and MH 318 than MH 421. RSI increased with increased DAE to high temperature; the increase more in Basanti (41.34%) followed by MH 318 (40.72%) than MH 421 (34.88%). MDA (21.75 to 34.44), TSCs (23.58 to 33.65 g g-1 DW) and proline content (3.11 to 17.77 mg g-1 DW) increased in all genotypes with increased DAE; the increase MDA, being more in leaves of Basanti (30.66 μ mol g-1DW) followed by MH 318 (28.97) than MH 421 (28.26). The TSCs and proline content were maximum in MH 421 (33.83 mg g-1 DW and 25.21 mg g-1 DW) followed by MH 318 (30.08 and 23.75) than Basanti (28.26 and 22.19) respectively. Moreover the RWC (%) and Osmotic adjustment (OA) were also better in MH 421under high temperature stress. High temperature induced the specific activity of antioxidant enzymes such as superoxide dismutase (SOD) (3.37 to 5.67), catalase (CAT) (7.40 to 11.48), peroxidase (POX) (10.09 to 21.95), ascorbate peroxidase (APX) (0.35 to 1.56), Glutathione peroxidase (GPX) (0.39 to 0.61), Glutathione transferase (GST) (0.22 to 0.80). The antioxidant i.e. total glutathione (409.49 to 513.09) and hydrogen peroxide (H2O2) (1.53 to 1.82) were enhanced while ascorbic acid (AAC) (25.20 to 16.88) and caroteonoid content (4.61 to 2.30) were decreased with increased DAE to high temperature. The specific activity of above said enzymes was maximum in MH 421 followed by MH 318 and minimum in Basanti at all the DAE to high temperature. The total glutathione, ascorbate and caroteonoid content were higher in MH 421 as compared to other genotypes while reverse trend was seen for H2O2 content.