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Chaudhary Charan Singh Haryana Agricultural University, Hisar

Chaudhary Charan Singh Haryana Agricultural University popularly known as HAU, is one of Asia's biggest agricultural universities, located at Hisar in the Indian state of Haryana. It is named after India's seventh Prime Minister, Chaudhary Charan Singh. It is a leader in agricultural research in India and contributed significantly to Green Revolution and White Revolution in India in the 1960s and 70s. It has a very large campus and has several research centres throughout the state. It won the Indian Council of Agricultural Research's Award for the Best Institute in 1997. HAU was initially a campus of Punjab Agricultural University, Ludhiana. After the formation of Haryana in 1966, it became an autonomous institution on February 2, 1970 through a Presidential Ordinance, later ratified as Haryana and Punjab Agricultural Universities Act, 1970, passed by the Lok Sabha on March 29, 1970. A. L. Fletcher, the first Vice-Chancellor of the university, was instrumental in its initial growth.

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2013 - 201514
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Subject: Molecular Biology and Biotechnology × End date: 2015 × Start date: 2013 × Type: Thesis × Thesis Type: M.Sc ×
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Now showing 1 - 9 of 14
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    ThesisItemOpen Access
    Micronutrient and SSR marker analysis of pearl millet [Pennisetum glaucam (L.) R. Br.] inbred lines
    (CCSHAU, 2015) Sangwan, Sonali; Yashveer, Shikha
    A set of thirty-six pearl millet inbred lines was used to study genetic variability and correlation analysis for seven morphological characters namely days to 50% flowering, plant height (cm), ear length (cm), ear diameter (cm), 1000 grain weight (g), grain yield per plant (g) and dry fodder yield per plant (g) along with micronutrient (Fe and Zn) status. The pearl millet inbred lines were evaluated in randomized block design with two replications in two environments during 2014. Analysis of variance for all the characters under study showed significant differences indicating the presence of genetic variability among the inbreds. The estimates of variability parameter for grain yield, contributing traits and Fe and Zn content revealed the high PCV value as compared to GCV value that suggests the role of environment in the expression of all these characters. An additive gene action was indicated for dry fodder yield per plant, grain yield per plant, Fe and Zn content by high to moderate heritability and genetic advance as per cent of mean values. Correlation analysis showed highly significant correlation between grain Fe and Zn content which signifies simultaneous improvement in the two traits. Grain yield per plant showed no significant negative correlation with Fe and Zn that suggests improvement in nutrient value without sacrificing yield. A set of 30 SSR primers was also used for molecular diversity assessment in 36 pearl millet inbred lines. Mean allele per locus and PIC obtained was 10.5 and 0.796, respectively. Cluster analysis distributed these genotypes into 9 clusters where 4 genotypes failed to fall in any of these clusters.
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    ThesisItemOpen Access
    Phenotyping and genotyping for salt tolerance of KHARCHIA65 X HD2285 F2 population in wheat (Triticum aestivum L.em.Thell)
    (CCSHAU, 2014) Suman; Shikha Yashveer
    Experiments were conducted to validate markers (Nax1 and Nax2) for salinity tolerance and phenotyping and genotyping of F 2 Population of cross Kharchia65x HD2285. Net house evaluation data showed enormous variation among Kharchia65x HD2285F 2 plants including plant height (cm), No. of tillers per plant, ear length (cm), No. of grains /ear, No. of spikelets/spike, No. of grains/spikelet, grain yield per plant, 1000 grain weight (g), biological yield/plant (g), harvest index (%) and germination percentage under salt stress condition. ). 90 plants were selected with high yield per plant and their tolerance to salt stress but only 20 plants were found to be Nax2 positive. All the 20 F 2 plants clustered in two major groups at the similarity coefficient of 0.43. Major group 1 consisted of parent HD2285 while major group II had Kharchia65 and all F 2 plants. This is the probably the first report where Nax2 has been validated in Kharchia65 and it clearly indicates that a linked marker like Nax2 could provide a valuable tool for breeding wheat with enhanced tolerance to salinity conditions.
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    ThesisItemOpen Access
    Phenotyping and microsatellite marker analysis for yellow rust in WH711 × WH542 F2 population of wheat (Triticum aestivum L.em.Thell)
    (CCSHAU, 2014) Vashisth, Medhavi; Dhillon, Santosh
    Stripe rust (yellow rust), caused by Puccinia striiformis Westend. f. sp. tritici Eriks., is one of the most devastating diseases of wheat throughout the world. Marker assisted breeding is the most economical and environmentally acceptable method to control stripe rust. The present investigation was carried out to evaluate F2 population derived from WH711 and WH542 varieties of wheat for reaction to yellow rust, traits contributing to yield and SSR analysis. WH711 (susceptible to yellow rust) × WH542 (resistant to yellow rust) F2 population when screened against yellow rust reaction, fitted in asegregating ratio of 9:3:3:1 for highly resistant, moderately resistant, moderately susceptible and highly susceptible plants, suggesting the digenic effect (two gene segregation) controlling the respective trait. Correlation analysis revealed that biological yield per plant (0.891) had the highest direct contribution towards grain yield per plant followed by number of tillers per plant (0.816). Out of 100 SSR markers used, 81 SSRs showed amplification, out of which 17 SSRs (including four Yr specific SSRs specific for Yr genes viz. Yr1, Yr16, Yr36, YrH52) produced polymorphic bands while 64 SSRs produced monomorphic bands. Size of amplified products in two different genotypes ranged from 86 bp to 288 bp. WH711 × WH542 plants showed heterozygosity of 38% and homozygosity of 62%. Though no marker was found linked to the rust resistance but NTSYS-PC UPGMA cluster tree analysis led to the grouping of 120 F2 plants in two major groups at a similarity coefficient of 0.55, major group I containing 22 F2 plants and WH711while major group II containing 97 F2 plants and WH542, thus indicating F2 population is more inclined towards WH542.
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    ThesisItemOpen Access
    Molecular marker analysis for bacterial blight resistance in cluster bean (Cyamopsis tetragonoloba (L.) Taub.)
    (CCSHAU, 2014) Amandeep Kaur; Yadav, Neelam R.
    Cluster bean (Cyamopsis tetragonoloba) is an important member of Leguminoseae family and is a rich source of galctomannan content making it an industrial crop. In this crop, there are almost no genomic resources or high quality co-dominant markers available for genetic analysis. At present there is no report of SSR markers targeting bacterial leaf blight resistance available for this crop but available EST database was used to develop EST-SSRs. Cluster bean EST sequences (16476) were retrieved and used to identify EST-SSRs using SSRIT tool. A total of 858 SSRs were identified with maximum proportion of trinucleotide repeats and least proportion of pentanucleotide repeats. CT/TC motif was most abundant. Eighty sequences related to pathogen stress response were identified out of which 17 SSRs were used for in silico primer designing. Twenty seven genotypes were selected for molecular marker analysis using these 11 EST-SSRs. Plants were also grown in net house for studying disease incidence. Two wild speices, C. senegelensis (18.55 ± 1.86 %) and C. serrata (16.30 ± 0.93 %) showed lowest disease incidence, while highest disease incidence was observed in case of Pusa Nav Bahar (73.23 ± 0.92 %). All the 11 EST-SSR markers were polymorphic in molecular analysis, showing different allele sizes in wild and cultivated genotypes. Diversity analysis suggested that two wild species formed a different group at a similarity coefficient of 0.75 including some of the cultivated genotypes. Four genotypes, C. senegelensis, HG 563, HG 75 and PNB, were selected for sequencing and multiple sequence alignment against HES 1401. The alignment suggested the presence of SNPs and In/ Dels in the sequences
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    ThesisItemOpen Access
    Marker-assisted selection of aerobic x Basmati segregating genotypes under aerobic conditions in rice (Oryza sativa L.)
    (CCSHAU, 2014) Khasa, Ritu; Jain, R.K.
    Experiments were conducted to evaluate segregating Pusa 1121 (Basmati) x MAS25 (aerobic) F 5 , MASARB25 (aerobic) x Pusa Basmati 1460 (Basmati) F4 and Pusa 1121 x MAS26 (aerobic) F 2 populations for various physio-morphological and/or root traits and allelic diversity for BAD2 (aroma) gene and microsatellite markers linked to the traits promoting aerobic adaptation. In all the three populations, enormous variation was observed for plant height, panicle length, effective number of tillers per plant, root length, root thickness, fresh and dry root weight, 1000 grain weight, grain length/breadth ratio and yield per plant. In these populations, significant positive correlation was observed between yield per plant with plant height, effective no. of tillers per plant, length/breadth ratio, 1000 grain weight, root length and/or root biomass. In 93 plants of these three populations, 62 plants had Basmati specific allele in homozygous condition, 12 plants had indica allele (homozygous condition) and 19 plants had both Basmati and indica allele (heterozygous condition) at BAD2 aroma locus. The NTSYS-pc dendrogram and 2D PCA were prepared using allelic diversity data and F 2 /F4 /F5 populations were inclined towards MAS26, Pusa Basmati 1460 and MAS25 respectively. A number of promising F 2/F 4 and F 5 plants have been selected, which had higher grain yield, root length and biomass greater than MAS25, MAS26 and MASARB25 and Basmati specific allele at BAD2 locus (either in homozygous or heterozygous condition) for further progeny analysis. Most of these selected plants had the desired allele for the markers reported earlier to be linked with the aerobic adaptation traits (RM205 and RM547 for root length on chromosome 8 and 9 respectively)
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    ThesisItemOpen Access
    Segregation for Iron and Zinc Content in F3 Populations derived from crosses between Aromatic and Mineral-Rich Indica Rice (Oryza sativa L.) Varieties
    (CCSHAU, 2014) Shishpal Singh; Sikka, V.K.
    Research had been carried out on 190 F 3 plants derived from mineral rich Indica and elite basmati rice variety. Out of 190, 50 plants were derived from Pusa Basmati 1 x Palman 579 and 140 plants were derived from Pusa Basmati 1 x HKR 95-157 cross. These F 3 plants were analyzed for mineral content and agronomical traits. A large variation was observed for plant height (100-168 and 90-163 cm), effective number of tillers per plant (5-19 and 7-30), panicle length (24.4-34.8 and 20.2-32), length/breadth (L/B) ratio (3.44-6.71 and 3.46-5.81), 1000-grain weight (18.1-30.7 and 11.4-29.6 g), yield per plant (3.9-40 and 8.11-60.12 g) in F 3 populations of Pusa Basmati 1 x Palman 579 and Pusa Basmati1 x HKR 95-157 respectively. Analysis of micronutrient content including Fe and Zn content revealed that the Fe and Zn contents in parents Pusa Basmati 1, Palman 579 and HKR 95-157 was 33.6, 16.5 µg/g, 363.53, 22.16 µg/g and 422.13, 17.4 µg/g respectively. The Fe and Zn content among the 50 Pusa Basmati 1 x Palman 579 F 3 plants ranged from 1.2-355.2 and 7.3-112.5 µg/g respectively whereas the Fe and Zn content in plants derived from 140 Pusa Basmati 1 x HKR 95-157 F 3 plants was 5.8-251.7 µg/g and 8.8-43.4 µg/g. For aroma out of 50 as much as 30 plants were found to be aromatic and out of 140, 94 plants were found to be aromatic in crosses Pusa Basmati 1 x Palman 579 and Pusa Basmati 1 x HKR 95 157 respectively. The F 3 plants were analyzed to get the plants which have a combination of desirable traits and 20 superior progenies with suitable traits had been selected.
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    ThesisItemOpen Access
    Phenotyping and molecular marker analysis of selected HKR47 x MAS26 (aerobic) F2 rice progenies
    (CCSHAU, 2013) Usha Rani; Jain, R.K.
    Experiments were conducted to evaluate segregating HKR47 x MAS26 (aerobic) F3 plants for various physio-morphological and root traits and allelic diversity for 7 microsatellite markers linked to the root traits. In HKR47xMAS26 F3 plants enormous variation was observed for plant height, panicle length, and number of tillers per plant, seeds per panicle, percent seed setting, root length, root thickness, root volume, fresh and dry root weight, 1000 grain weight, grain length/breadth ratio and yield per plant. The NTSYS-pc UPGMA cluster tree analysis led to the grouping of 46 F3 plants in two major groups at a similarity coefficient of 0.5. All the 46 F3 plants clustered in two major groups at the similarity coefficient of 0.50. Major group I consisted of HKR47 and 16 F3 plants while the major group II had MAS26 and 30 F3 plants. A number of promising F3 plants have been selected, which had higher grain yield, root length and biomass greater than the parental rice varieties (MAS26 and HKR47)for further progeny analysis.
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    ThesisItemOpen Access
    Effect of medium constituents on micro propagation of sugarcane cultivar (cos-7717)
    (CCSHAU, 2013) Khichi, Poonam; Chowdhury, V. K.
    The experiments were conducted to study the effect of various media, gelling agents, carbon sources and photoperiods on in- vitro establishment, multiplication and rooting response in sugarcane cultivar (CoS-7717) using apical shoot tips as explants. For in- vitro establishment shoot tips were cultured on different media (Murashige and Skoog , Gamborg et al,White , Nitsch and Nitsch media) on BAP (0.5mg/l)+KIN(0.5mg/l) followed by establishment on MS medium supplemented with different c-source (Table Sugar, Sucrose , Fructose , Maltose , Glucose), gelling agents (Agar-Agar, Agarose , Gelerite , Guwargum , Isabgol) and varying photoperiods (16, 20 and 24h). Maximum number of shoots/explant (2±0) were reported on MS media fortified with BAP (0.5mg/l) + KIN(0.5mg/l), sucrose (30.0g/l) and agar-agar (8.0g/l) as a gelling agent using photoperiod of 16h. For in- vitro multiplication, the sprouted buds were transferred onto MS media containing different concentrations of growth regulators alone and in combination i.e. BAP, KIN, NAA, TDZ, SPM and PUT along with different carbon source (sugars), gelling agents and varying photoperiods (16, 20 and 24h). The maximum number of shoots (18.0±2.1) and shoot length (10.8±0.30cm) was reported on MS media fortified with BAP (0.25mg/l) +KIN (0.25mg/l) +NAA (0.1mg/l), supplemented with tablesugar (30.0g/l) and agar-agar(8.0g/l) under 16h of photoperiod. In vitro raised shoots produced 100% rooting on full strength MS medium supplemented with 5.0mg/l NAA and 50g/l sugar within 4 days of inoculation. Maximum survival (100%) of transplanted shoots was observed in Sand+ Soil+ Vermicompost (1:1:1) under green house conditions. Hardened plantlets were successfully transferred to the field conditions.
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    ThesisItemOpen Access
    Molecular analysis of male & female genotypes of Simarouba glauca DC using ISSR markers
    (CCSHAU, 2013) Yadav, Poonam; Dhillon, Santosh
    Simarouba glauca is a tree born oilseed crop. It belongs to the family Simaroubaceae and is a native of El Salvador (Central America). The seeds of Simarouba are economically very important since they contain 55-60% edible oil. Simarouba is polygamodioecious with three types of plants namely pistillate (Female flowers), Staminate (Male flowers) and andromonoecious (male dominated bisexual flowers). As the waiting time from sowing to flowering is long, so the determination of sex of Simarouba seedling prior to flowering stage is necessary. Molecular markers are effectively and commonly being used in studying molecular polymorphism and genetic diversity in species. The present study was undertaken to assess molecular polymorphism among ten male, ten female and five bisexual genotypes of Simarouba glauca using ISSR markers. In the ISSR analysis of 25 genotypes using 40 ISSR primers, total 170 amplification products were obtained, out of which 34 are monomorphic and 136 are polymorphic. The polymorphism percentage ranged from 57.14% to 100%. The average polymorphism across all the 25 genotypes was found to be 87.6%. Overall size of PCR amplified products ranged between 150 bp-1700 bp. The similarity indices between different genotypes ranged from 0.43 to 0.93. These results indicate the presence of large genetic variation among the genotypes studied. Out of 40 ISSR primers that were surveyed to identify sex of Simarouba glauca, primer ISP815 was found to be male specific. This primer can be further converted into a SCAR marker which is more robust and will be of potential use in selecting plants of desired sex. The present study also demonstrates the utility and importance of ISSR markers in genetic diversity analysis and genotyping in Simarouba glauca.