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Chaudhary Charan Singh Haryana Agricultural University, Hisar

Chaudhary Charan Singh Haryana Agricultural University popularly known as HAU, is one of Asia's biggest agricultural universities, located at Hisar in the Indian state of Haryana. It is named after India's seventh Prime Minister, Chaudhary Charan Singh. It is a leader in agricultural research in India and contributed significantly to Green Revolution and White Revolution in India in the 1960s and 70s. It has a very large campus and has several research centres throughout the state. It won the Indian Council of Agricultural Research's Award for the Best Institute in 1997. HAU was initially a campus of Punjab Agricultural University, Ludhiana. After the formation of Haryana in 1966, it became an autonomous institution on February 2, 1970 through a Presidential Ordinance, later ratified as Haryana and Punjab Agricultural Universities Act, 1970, passed by the Lok Sabha on March 29, 1970. A. L. Fletcher, the first Vice-Chancellor of the university, was instrumental in its initial growth.

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2010 - 20142
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Subject: Molecular Biology and Biotechnology × Author: Naveen Kumar × Start date: 2008 × Type: Thesis ×
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    ThesisItemOpen Access
    Selection of high-yielding iron-rich PAU201/Palman579 segregating rice (Oryza sativa L.) lines using conventional and molecular marker techniques
    (CCSHAU, 2014) Naveen Kumar; Chowdhury, V.K.
    Molecular markers provide novel tools for linkage mapping of QTLs of target traits and can greatly enhance the efficacy of breeding programs to improve mineral (iron and zinc) density in rice. F3, F4, BC1F2 and BC1F3 populations derived from the cross between high-yielding (PAU201) and iron-rich (Palman 579) indica rice varieties displayed large variation for various physio-morphological traits including grain yield per plant and mineral (iron and zinc) contents. Iron and zinc content varied from 0.9- 149.9 and 0-143.1 μg/g respectively in all the four populations (F3, F4, BC1F2 and BC1F3). Transgressive segregation for grain iron content was noticed in F3 population with one of the plants having exceptionally higher iron (746.8 μg/g) content. Phenotypic correlation analysis showed positive correlation (r=0.281) between grain iron content and zinc content in BC1F2 population but not in F3, F4 and BC1F3 populations. Grain yield per plant showed significant positive correlation with plant height and effective number of tillers/plant in all the four populations. A DNA fingerprint database of 33 PAU201 × Palman 579 F4 plants was prepared using 61 polymorphic SSR markers distributed on the entire genome of rice. NTSYS-pc UPGMA tree cluster analysis and two-dimensional PCA scaling showed scattering of the F4 population between the two distinct parental genotypes; the population was inclined towards Palman 579. The distribution of PAU201 and Palman 579 specific alleles among the PAU201 × Palman 579 F4 plants were determined and SSR data was used to identify QTLs for grain mineral content and various agronomical traits. A total of 128 alleles were identified in 33 PAU201 x Palman 579 F4 plants and three new recombinant alleles (different that those in parent rice varieties) were identified. Composite interval mapping (CIM) analysis by Win QTL cartographer 2.5 revealed a total of six QTLs for mineral content (five for Fe and one for Zn) in rice grains on chromosome 5, 6, 7 and 9; and sixteen QTLs for various agronomical traits (plant height, tillers per plant, yield per plant, Grains per panicle and 1000-grain weight). As many as 33 F4 and 25 BC1F3 promising plants having higher grain yield as well as mineral content have been selected for further progeny analysis.
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    ThesisItemOpen Access
    To develop an efficient micropropagation protocol for Stevia rebaudiana
    (CCSHAU, 2010) Naveen Kumar; Chowdhury, V.K.
    The present investigation has been undertaken to: (i) study the effect of various concentrations of different growth regulators on in vitro shoot multiplication and growth in Stevia rebaudiana and (ii) to develop the micropropagation protocol for efficient and quick multiplication of Stevia rebaudiana. The shoot-tip and nodal segments were used as explants. Different sterilization protocols were tried for surface sterilization of the explants in Stevia rebaudiana. The nodal and shoottip explants were surface sterilized with 0.2% bavistin for 20 min and 70% alcohol treatment for 50 sec followed by 0.1% HgCl2 for 3 min which resulted in 100% sterilization of the explants. The nodal and shoot-tip explants were best regenerated on MS medium+BAP 2.0mg/l. The per cent explants regenerating shoots on nodal and shoot –tip explants were 90% and 50% respectively. The average no. of shoots was 2.00 and 3.2 from nodal and shoot-tip explants respectively. The primary proliferated cultures were transferred on to various MS media supplemented with different concentration and combination of growth regulators for shoot multiplication. The best shoot multiplication medium was MS medium supplemented with BAP (0.3mg/l) + Kinetin (0.3 mg/l), on which an average 50.20 number of shoots developed from nodal and shoot-tip explants after 40 days on culture medium. The other growth regulators viz. zeatin, TDZ and GA3 were also tried to check the effect on in vitro shoot multiplication at different interval of time but the response was not satisfactory. The multiple shoots were surgically separated and transferred on to various rooting media. The frequency of root regeneration was 100% on half MS medium containing IBA (0.5 mg/l) while the rooting frequency was 50.00% on half MS medium containing IAA (2.0 mg/l) and 42.86% on ½ MS+IAA (0.1 mg/l). Regenerated plantlets were shifted to pots containing sterile soil and sand mixture (3:1) for propagation. The survival percentage of plants after hardening and transfer to potted soil was 80%.