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Chaudhary Charan Singh Haryana Agricultural University, Hisar

Chaudhary Charan Singh Haryana Agricultural University popularly known as HAU, is one of Asia's biggest agricultural universities, located at Hisar in the Indian state of Haryana. It is named after India's seventh Prime Minister, Chaudhary Charan Singh. It is a leader in agricultural research in India and contributed significantly to Green Revolution and White Revolution in India in the 1960s and 70s. It has a very large campus and has several research centres throughout the state. It won the Indian Council of Agricultural Research's Award for the Best Institute in 1997. HAU was initially a campus of Punjab Agricultural University, Ludhiana. After the formation of Haryana in 1966, it became an autonomous institution on February 2, 1970 through a Presidential Ordinance, later ratified as Haryana and Punjab Agricultural Universities Act, 1970, passed by the Lok Sabha on March 29, 1970. A. L. Fletcher, the first Vice-Chancellor of the university, was instrumental in its initial growth.

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Subject: Genetics and Plant Breeding × Author: Geeta Devi × End date: 2017 × Start date: 2017 × Thesis Type: Ph.D ×
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    ThesisItemOpen Access
    Genetics and molecular studies for Alternaria blight, seed yield, its component characters in Indian mustard [Brassica juncea (L.) Czern & Coss.]
    (CCSHAU, 2017) Geeta Devi; Thakral, N.K.
    Alternaria blight is one of the major biotic stresses in Indian mustard and reduces yield by 32-38% worldwide. The present study was undertaken with the objectives (i) To study the nature and magnitude of gene effects responsible for seed yield, its component characters, and Alternaria blight. (ii) To determine general and specific combining ability effects for different traits. (iii) To study polymorphism in parents using molecular markers. To achieve these objectives, 13 Indian mustard genotypes were sown in oilseed research area during 2014-15 and develop 30 TTC families during 2015-16 in RBD with 3 replications in triple test cross mating design. Observations were recorded on 12 morphological parameters. For second experiment (line x tester) 13 Indian mustard genotypes were sown during 2014-15 and develop 30 crosses during 2015-16 in RBD with 3 replications. Observations were recorded on 11 morphological parameters. SSR analysis was also carried out using 105 primers. ANOVA revealed significant differences among 13 genotypes for all the traits studied. Based upon triple test cross analysis all characters are studied were observed to posses epistasis, in both the environments except number of seeds per siliqua and oil content in late sown condition. Days to maturity, main shoot length, number of siliquae on main shoot, siliqua length, no of seeds per siliqua, 1000 seed weight and oil content in both the environments, plant height, no. of primary branches and Alternaria blight intensities under late sown environment are governed by the additive gene effects. In contrast, no. of secondary branches and seed yield per plant in both the environments, no. of primary branches and Alternaria blight intensities in timely sown environment are controlled by the dominance gene effects. On the basic of line x tester analysis mean squares due to crosses were also significant for all the traits which depicted presence of heterosis for all the traits, except for number of seeds per siliquae and oil content. Main shoot length, siliquae on main shoot and seed yield per plant are controlled by additive genetic variance which revealed that these traits may be used for selection. Plant height, days to maturity, no of primary branches, no. of secondary branches, siliqua length, no of siliqua on main shoot, no of seed per siliquae and oil content are govrned by dominant genetic variance. GCA effects revealed that RH9304 and RH 1378 were good general combiner for majority of the characters. High GCA effects are related to additive gene effects or additive x additive interaction which represent the fixable genetic component of variation. Hence these parents could be efficiently used for exploiting seed yield. For seed yield the crosses RH1368 x RH1372, RH0923 x RH1371 and NPJ112 x RH1378 were identified as promising on the basis of their high per se performance, along with high significant SCA effects and these crosses are capable of producing progenies with high transgressive effects. These crosses could be extensively used in breeding programme to develop superior segregants could be derived in further breeding programmes. Molecular analysis was done using 105 SSR primers and 39 primers showed polymorphism. The NTSYS-PC UPGMA cluster tree analysis led to the grouping of sixteen genotypes into two major clusters with similarity index 0.55. Out of 105 primers, primer ENA2 have maximum PIC value and indicating that this primer show maximum contribution for diversity analysis.