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Chaudhary Charan Singh Haryana Agricultural University, Hisar

Chaudhary Charan Singh Haryana Agricultural University popularly known as HAU, is one of Asia's biggest agricultural universities, located at Hisar in the Indian state of Haryana. It is named after India's seventh Prime Minister, Chaudhary Charan Singh. It is a leader in agricultural research in India and contributed significantly to Green Revolution and White Revolution in India in the 1960s and 70s. It has a very large campus and has several research centres throughout the state. It won the Indian Council of Agricultural Research's Award for the Best Institute in 1997. HAU was initially a campus of Punjab Agricultural University, Ludhiana. After the formation of Haryana in 1966, it became an autonomous institution on February 2, 1970 through a Presidential Ordinance, later ratified as Haryana and Punjab Agricultural Universities Act, 1970, passed by the Lok Sabha on March 29, 1970. A. L. Fletcher, the first Vice-Chancellor of the university, was instrumental in its initial growth.

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2002 - 2009132010 - 20113
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Subject: Genetics × Start date: 2002 × Thesis Type: M.Sc ×
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Now showing 1 - 9 of 16
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    ThesisItemOpen Access
    Cytogenetic studies on induced mutants of cotton (Gossypium spp.)
    (College of Agriculture Chaudhary Charan Singh Haryana Agricultural University Hisar, 2003) Usha; Singh, K. P
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    ThesisItemOpen Access
    In vitro propagation of female jojoba [Simmondsia chinensis (Link) Schneider] plants
    (College of Agriculture Chaudhary Charan Singh Haryana Agricultural University Hisar, 2002) Rani, Meenu; Uppal, S
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    ThesisItemOpen Access
    Genetic Studies on Advanced Lines for Yield and Qualtiy Components in Wheat (T. Aestivum L. Em. Thell)
    (College of Agriculture Chaudhary Charan Singh Haryana Agricultural University Hisar, 2002) Malik, Parul; Rana, O. P. S.
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    ThesisItemOpen Access
    Technology Assessment in Haryana - An Analytical Study
    (College of Agriculture Chaudhary Charan Singh Haryana Agricultural University Hisar, 2002) Singh, Jasbir; Narwal, R. S
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    ThesisItemOpen Access
    Phenotyping of CSR-10 X HBC-19 RILs in rice
    (CCSHAU, 2006) Pummy Kumari; Ahuja, Uma
    The present investigation was undertaken to characterize 231 recombinant inbred lines (F7) from a cross of CSR-10 X HBC-19 for agronomic and grain quality traits. RILs were grown in augmented design at experimental area of CCS HAU Rice Research Station, Kaul during kharif season of 2004-05. Data was recorded on various morphological traits as plant height, tillers per plant, grain yield per plant, 1000-grain weight and quality traits as hull colour, grain colour, hulling and milling percentage, raw grain dimensions, cooked grain dimensions, aroma, alkali value and amylose content. Data was analyzed for correlation coefficient and regression analysis to estimate the levels of variation and association among agronomic and grain quality traits. RILs of F7 population showed large variation for all the agronomic and grain quality traits. High genotypic (GCV) and phenotypic (PCV) coefficient of variation were observed for maximum number of traits except thickness of raw grain and hulling percentage. GCV occupied major extent of PCV. Correlation coefficient revealed that yield per plant was associated with 1000-grain weight and plant height but not with grain quality traits, indicating that yield can be combined with grain quality. However, a balance has to be maintained as 1000-grain weight showed negative association with grain quality traits. In addition to parental hull colours, different colours as light brown, dark brown, red and yellowish green were observed. Similarly, in addition to parental creamish grains, various colours as light brown, green, medium red and reddish brown were observed. Mildly aromatic entries were observed in addition to highly aromatic, non-aromatic parents and RILs. Frequency distinbution indicated transgressive variation indicating polygenic control for all traits. High variability observed for all agronomic traits and hull colour, grain colour, hulling and milling percentage, length of raw grain, length: breadth ratio and elongation ratio make these RILs ideal population for linkage studies and molecular marker analysis.
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    ThesisItemOpen Access
    Molecular profiling of mungbean genotypes vigna radiata (L.) wilczek using RAPD analysis
    (CCSHAU, 2007) Madhu; Subhadra Singh
    A study on 39 genotypes of mungbean representing a cross-section of variability was undertaken to assess RAPD polymorphism and to estimate the matrix of Jaccard’s similarity coefficient. A total of 411 amplified products comprising 382 (92.9%) polymorphic and 29 (7.1%) monomorphics markers were observed using 30 RAPD primers. The number of bands generated per primer ranged from 7 to 20 bands with a mean of 13.7 bands per primer. The primers showed a large variation in the frequency of polymorphic bands (0.026 - 0.974) and in number of banding patterns (3 – 35). Efficiency of primers were analyzed using Discrimination power (D), Diversity index (DI), Resolving power (Rp) and Marker index (MI). All efficiency parameters except DI could identify most efficient (S-1 and S-2) and the least efficient (S-30) primers. Both discrimination power (D) and resolving power (Rp) parameters appear to be good measure of the usefulness of primers to identify cultivars. The experimental results obtained for real efficiency of the primer agreed well with that of theoretical expectation based on independent hydpothesis of primers. Genetic similarity coefficient ranged from 40.8 per cent to 90.3 per cent indicating moderate level of genetic variability among genotypes. Some of the genotypes such as K 851, Pusa Ratna, MH 98-1, Asha, MH 96-1 Muskan, Koppergaon, K 851, Pusa Baishakhi, ML 1108, PDM 262, ML 682, Pusa 9072 and ML 818 exhibited higher genetic similarity with rest of the genotypes. ML 131 was the most diverse genotype. Other genotypes namely ML1349, Pant M-4, Greengram Co-5, SML 32, UP 99-3, ML 1333, UPM-98 also exhibited higher diversity. The dendrogram constructed from Jaccard’s similarity coefficient showed three main groups. The group A consists of 26 genotypes while group B and C consist of three and two genotypes only. The mungbean genotypes evolved at PAU, Ludhiana (Punjab) and in Uttar Pradesh were comparatively more diverse among themselves than the group of genotypes from Hisar (Haryana) and IARI (Delhi). The grouping of some genotypes corresponded with their pedigree information but failed in other cases. The same was true with the geographical origin vis-à-vis grouping of genotypes. Mantel’s test between the cophenetic matrix and the original matrix (z=r=0.923) revealed a good fit of cluster analysis performed. The pattern of grouping in cluster analysis was also reflected in Principal Coordinate Analysis (PCoA) with minor differences. The three coordinate PCoA could account 73.68 per cent of total variability in RAPD markers. The high level of RAPD polymorphism observed in mungbean may be useful for variety protection and monitoring the genetic diversity.
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    ThesisItemOpen Access
    Evaluation of recombinant inbred lines (RILs) of bread wheat for thermo response at seedling stage
    (CCSHAU, 2008) Sharma, Vikas; Yadava, Ramesh Kumar
    The present study has been conducted to Evaluation of recombinant inbred lines (RILs) of bread wheat for thermo response at seedling stage. The seed weight (g), seed density (mg ml-3), shoot length (cm), root length (cm), seedling length, fresh weight (mg), dry weight (mg), vigour (I), vigour (II) and germination % in 100-RILs of Harrier x S308 wheat population were observed at three temperature conditions (15º, 20º and 25ºC) with two replications. The estimates of mean, components of genetic parameters (GCV, PCV,h2, genetic advance etc.) and correlation coefficients along with divergence analysis has been worked out after the establishment of substantial amount of variability in the present material. The estimates of h2 (BS) were high for almost for all traits. Genetic advance expressed as % of mean revealed the maximum scope of improvement is for shoot length at 15ºC. Correlation coefficient was indicated to be highest for dry weight and fresh weight at all the three temperatures, and was followed by seedling length with shoot length and that with vigour I at 15ºC; fresh weight with vigour II at 20º and 25ºC and that of dry weight with vigour I at 20ºC and with vigour II at 25ºC. Euclidean Clustering revealed that these RILs could be grouped in 9 clusters at 15ºC, where as in 8 clusters at 20ºC and 25ºC. Only one combination of RILs HS-135 & -136 had the tendency of grouping under same cluster across all the temperatures. Cluster II had maximum xii number of genotypes (24) at 15ºC whereas cluster III at 20ºC, and 25ºC had 20 and 19 genotypes respectively. The cluster VIII had the minimum number of RILs (two) at 15ºC where as four at both 20ºC and 25ºC in each case. At 15ºC RILs in cluster I were better for SL, RL, Se L, VI in cluster III were better for FW, GP, and that of cluster VIII were better for SW, SD, DW, VII as revealed by cluster means, RILs grouped in cluster I at 20ºC were better for RL, Se L, FW, DW, VI, VII; and that of cluster II were better for SW, SL, whereas in case of cluster III were better for SD, GP on the basis of cluster means. Likewise, RILs in cluster I were better for SW, SD; of cluster II being better for FW, DW, VII, GP, and that of cluster III as better for SL, RL, Se L, VI , as indicated by cluster means at 25oC. Consistently higher mean values at all the three temperatures has been recorded in HS 104 for fresh weight, HS 3 for vigour I & HS 51 for germination %, whereas the RIL HS 98 had consistently lower means in case of fresh weight, dry weight and vigour II, HS 99 for shoot length & HS 86 for dry weight at all the three temperatures. RILs specific for improving the traits, shoot length, root length, seedling length, fresh weight and the various seedling vigour parameters of wheat under diverse temperature conditions have been identified.
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    ThesisItemOpen Access
    Genetic and symbiotic characterization of poly-β-hydroxybutyrate production in Rhizobium and Bradyrhizobium spp. nodulating mungbean [Vigna radiata (L.) Wilczek]
    (CCSHAU, 2008) Sandeep Kumar; Yadav, A. S.
    Twenty-one WT strains of Rhizobium/Bradyrhizobium sp. (Vigna) were isolated purified and tested for their ability to form nodules on the mungbean plants. These WT strains were screened for PHB production on Beringer’s MM with Nile blue A under UV light. The two WT strains MBR 16 and MBR 25 produced high amount of PHB and these were used to isolate PHB mutants. Three types of mutants were isolated and characterized as MHt, MM and ML mutants. The MHt mutants produced highest amount of PHB, whereas ML mutants produced very low amount of PHB. The Maximum amount of PHB was found in MBR 16 MHt 1 i. e. 1.24 g/l. The antibiotic resistance pattern of parent strains and their mutants was almost same. The symbiotic effectivity of parent strains and their mutants on mugbean plants showed that the MHt mutants had higher shoot dry weight and total shoot nitrogen than the MM and ML mutants. A highly significant positive correlation was found between the amount of PHB produced and shoot dry weight and total shoot nitrogen of the munbean plants.
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    ThesisItemOpen Access
    Studies on wheat-azotobacter interaction under pot house conditions
    (CCSHAU, 2008) Kusum; Vasudava, Manjula
    To study the varietal behaviour of wheat with regard to different strains of Azotobacter chroococcum, as experiment was conducted in which six varieties of wheat such as C-306, PBW-343, WH-283, WH-542, WH-147 and WH-711 were treated with two mutants of A. chroococcum Mac27 and HT54 at three levels of fertilizer such as O kg N ha-1, 90 kg N ha-1, 120 kg N ha-1 in terms of agronomical parameters under pot house conditions. Response of wheat varieties was studied towards A. chroococum in plant height, seed yield, total nitrogen content and total biomass. Variety C-306 showed best response towards both the strains of A. chroococcum. Mac27 showed better response as compared to HT54. Root exudates of these wheat varieties were analyzed for chemotactic behaviour and other components such as sugars, amino acids, phytohormones and organic acids. Glutamic acid and some other unidentified amino acids were detected in both inoculated and uninoculated wheat varieties. Succinic acid was present in wheat varieties WH-711 and WH-147. Maleic acid and some unidentified organic acids were detected in inoculated and uninoculated wheat varieties C-306, PBW-343, WH-283 and WH-542.