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Chaudhary Charan Singh Haryana Agricultural University, Hisar

Chaudhary Charan Singh Haryana Agricultural University popularly known as HAU, is one of Asia's biggest agricultural universities, located at Hisar in the Indian state of Haryana. It is named after India's seventh Prime Minister, Chaudhary Charan Singh. It is a leader in agricultural research in India and contributed significantly to Green Revolution and White Revolution in India in the 1960s and 70s. It has a very large campus and has several research centres throughout the state. It won the Indian Council of Agricultural Research's Award for the Best Institute in 1997. HAU was initially a campus of Punjab Agricultural University, Ludhiana. After the formation of Haryana in 1966, it became an autonomous institution on February 2, 1970 through a Presidential Ordinance, later ratified as Haryana and Punjab Agricultural Universities Act, 1970, passed by the Lok Sabha on March 29, 1970. A. L. Fletcher, the first Vice-Chancellor of the university, was instrumental in its initial growth.

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Subject: Genetics ร— Author: Gollen, Babita ร— Start date: 2003 ร— Type: Thesis ร—
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    ThesisItemOpen Access
    Genetic analysis of grain filling components using molecular markers in RILs of bread wheat (Triticum aestivum)
    (CCSHAU, 2013) Gollen, Babita; Subhadra Singh
    A total of 103 F10 RILs of bread wheat derived from HUW-510 x WH-730 were evaluated for their grain filling process under normal sown (NS) and late sown (LS) field experiments conducted in Rabi seasons of 2010-11 and 2011-12.An รก-design in replicates with 15 block each was used for evaluation. The grain filling was studied by sampling the developing grains at 5 - day intervals after days to anthesis (DAA) till physiological maturity. The process of grain filling was characterized by estimating 3 parameters of grain development, namely final grain weight (W), maximum grain filling rate (R) and grain filling duration (GFD) by fitting non-linear logistic regression. Highly significant genotypic differences existed for all the three grain filling traits. However, a comparisons of : RILs with that of parents, between parents and among RILs revealed gene dispersion between parents. This inference was substantiated by the observation of transgressive segregants in both positive and negative directions for all the traits in the RIL population. Selective genotyping method of QTL detection was used and a total of 300 SSR primers were screened in a sub-population of 37 RILs and 112 primers were found to be polymorphic. Linkage map was constructed using 99 unambiguous polymorphic markers. QTL detection for W, R, GFD and also TGW and KPS was performed using single marker analysis (SMA) and composite interval mapping (CIM). A total of 61 putative QTLs using SMA and a total of 23 major QTLs were detected using CIM. Some QTLs in coupling and some in repulsion phase were observed and explained positive and negative associations respectively between traits. A number of QTLs for increasing the grain filling rate (R) and reducing the grain filling duration (GFD),were found to be useful to counteract the terminal heat stress . These QTLs may be exploited for genetic improvement of the wheat. Markers Xgwm95 and barc21 were found to be closely linked to QTLs for R and GFD and can be used for MAS after validation.
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    ThesisItemOpen Access
    Combining ability analysis for spike traits and other yield attributes in bread wheat (Triticum aestivum L. em Thell)
    (CCSHAU, 2009) Gollen, Babita; Yadav, R.K.
    Line x tester analysis involving twelve diverse wheat genotypes (HD2009M, R-6, R-49, HT90, HJP81, SG215, DI717, HS-11, HS-17, HG-2, Rm-Ts13 and HS-27 as lines in combination with six genotypes (WH711, PBW502, Rm-Ts10, Rm-Ts17, C591 and RVG) as testers was conducted by raising the experimental material at the research farm of the Department of Genetics during Rabi 2008. It has been exhibited that PCV is higher than the respective GCV and however, it is slightly lower for most of the characters thus indicating a high magnitude of heritability estimate in broad sense for most of the characters in general. Estimates of combining ability and magnitude of association between various character combinations have been worked out. The results indicated the existence of adequate genetic variation for all the characters studied. Both additive and non-additive type of genetic variance prevailed. C591 and Rm-Ts10 among the testers and HD2009M, HS-17, HG-2, DI717, R-6 and SG215 among the lines were found as good general combiners for one or the other characters. HS-17 for days to heading, HG-2 for plant height and peduncle length, R-6 for 100-grain weight, DI717 for number of grains per spike, SG215 for yield per plant have high GCA effects. Higher SCA effect was observed in the cross HD2009M x PBW502 followed by HS-27 x Rm-Ts10 and HG-2 x WH711 for the character number of grains per spike indicating the preponderance of non-additive gene action in these crosses. WH711 had positive SCA effects in six, PBW502 in nine, Rm-Ts10 in twelve,Rm-Ts17 in nine , RVG in ten and C591in twelve cross combinations. Most of the cross combination involve either both lines and testers or one of the parents as showing good combining ability thus indicating the importance of both additive and non-additive type of gene action. The coefficient for genotypic correlation for most of the character combination was higher in magnitude as compared to corresponding phenotypic correlation coefficient indicating the strong inherent nature of the traits. Grain yield per plant had a strong positive association with days to heading, plant height, peduncle length, tillers per plant, spike biomass and weight of grains per spike by considering both homozygous and heterozygous nature of material. Only few cases of correlation coefficients for the character combinations as per the pattern of heterozygous material has been visualized from the homozygous material which reveals that most traits are under the influence of polygenes and the heterozygous combination for them as has been obtained in various forms depending upon the parents. The informations generated from the present investigation may be utilized by involving good general combiners and the specific crosses that are likely to transgrants for various characters individually or in combination and appropriate breeding approach has been suggested.