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Chaudhary Charan Singh Haryana Agricultural University, Hisar

Chaudhary Charan Singh Haryana Agricultural University popularly known as HAU, is one of Asia's biggest agricultural universities, located at Hisar in the Indian state of Haryana. It is named after India's seventh Prime Minister, Chaudhary Charan Singh. It is a leader in agricultural research in India and contributed significantly to Green Revolution and White Revolution in India in the 1960s and 70s. It has a very large campus and has several research centres throughout the state. It won the Indian Council of Agricultural Research's Award for the Best Institute in 1997. HAU was initially a campus of Punjab Agricultural University, Ludhiana. After the formation of Haryana in 1966, it became an autonomous institution on February 2, 1970 through a Presidential Ordinance, later ratified as Haryana and Punjab Agricultural Universities Act, 1970, passed by the Lok Sabha on March 29, 1970. A. L. Fletcher, the first Vice-Chancellor of the university, was instrumental in its initial growth.

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Subject: Entomology × End date: 2020 ×
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    ThesisItemOpen Access
    Biochemical changes in genetic and induced systemic resistance due to infection of Albugo candida (Pers. ex. Lev) in Indian mustard
    (CCSHAU, Hisar, 2020-03) Annu; Rathi, A. S
    White rust disease incited by Albugo candida is an important disease of Indian mustard (Brassica juncea) in India particularly in northern and North eastern states. Keeping in view the enormous losses caused by the pathogen, the present investigation has been devised on biochemical changes due to infection of Albugo candida in different Indian mustard genotypes, induction of systemic resistance by non conventional chemicals, identification of slow white rusting genotypes and role of micronutrients in defence against white rust under laboratory, screen house and field conditions at Chaudhary Charan Singh Haryana Agricultural University, Hisar. The results of biochemical analysis revealed that total phenolic content, PO, PPO, PAL and chitinase activity were found higher in healthy leaves of resistant genotypes (BIOYSR, EC399299) as compared to moderately resistant genotypes (EC399301, DRMRIJ-31) and susceptible genotypes (RH 30, RH 8812) at 35-40 DAS. There was steep increase in all the biochemical constituents at the time of disease initiation ie. at 45-50 DAS. However, increase was more in inoculated environment as compared to uninoculated environment. At maximum disease severity i.e.70-75 DAS, there was decline in all the biochemical constituents in all genotypes under both the environments. Decrease was more in susceptible genotypes indicating the exhaustion of these biochemical constituents which otherwise consider necessary to trigger genetic resistance through pathways. Efficacy of six non conventional chemicals were tested with different concentrations for induction of resistance against white rust. None of the non conventional chemical completely controlled the white rust disease even at higher concentration of 100 μg/ml. However, a maximum control was also recorded by BTH at 100 μg/ml concentration after six weeks of challenge inoculation by the pathogen followed by SA in both the variety (RH 30 and RH 0749) under screen house conditions. BTH has shown maximum induction of phenolic content, PO, PPO, PAL and chitinase activity at three days after inoculation (DAI) in both the varieties at 100 μg/ml concentration followed by SA. However, MgSO4 has shown minimum induction of these activities at three DAI in both the varieties.Out of 22 Indian mustard genotypes screened for identification of slow white rusting component, 9 genotypes exhibited longer incubation and latent periods, minimum number of pustules per leaf, minimum size of pustule, minimum sporulation, minimum disease severity and minimum AUDPC indicating slow white rusting behaviour. Among the different treatments of mcronutrients alone and in combinations, soil application of Gypsum + Borax + CuSO4 + Na2MoO4 at 30 DAS as basal dose recorded minimum white rust severity of 31.1% and staghead incidence of 4.44% and highest seed yield of 2228.55 kg ha-1 as compared to the 45.83% white rust severity and 6.09% staghead incidence and seed yield of 1779.79 kg ha-1 in control. Maximum number of primary branches per plant (5.83), secondary branches per plant (12.33), main shoot length (90.93 cm), siliquae on main shoot (62.60) and seeds per siliquae (12.53) were also observed with above treatment. Plant height, siliqua length, oil content and 1000 seed weight did not vary significantly among all the treatments.
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    ThesisItemOpen Access
    Effect of feeding different carbohydrate and pollen sources on Apis mellifera L. colonies during dearth period
    (CCSHAU, Hisar, 2020-01) Rathee, Mandeep; Chaudhary, O. P
    The present investigations were carried out at the apiary located at College of Agriculture, Kaul, CCS HAU, Hisar to evaluate the effect of feeding different carbohydrate sources with and without pollen on Apis mellifera L. colonies during 2017 dearth period. A total of 30 A. mellifera bee colonies in standard Langstroth hives were tested under two sets of experiments i.e. without pollen and with pollen (80 g/colony/week), and each set had five treatments [4 carbohydrates sources (500 g/colony/week): sugar syrup, jaggery, liquid glucose, high fructose rice syrup and 1 control (no feeding)]. During 2018, jaggery was ruled out and remaining carbohydrate sources were fed @ 750 g/colony/week with high protein (23%) and low protein pollen (18%). Observations were recorded on pollen income, colony growth parameters, colony weight, incidence of insect-pests, diseases and mites, robbing, bee mortality, deformities, feed consumption, chemical and organoleptic analysis of honey, survey of bee flora, melissopalynology of pollen loads, their protein content and calorific value. Economics of feeding treatments was evaluated alonth with colony performance in ensuing honey flow seasons. Based on two years of studies, it was concluded that experimental area had severe dearth of nectar and pollen providing floral plants from June to October during both the years. In the absence of feeding, A. mellifera colonies could not survive beyond 57 and 64 days during 2017 and 2018, respectively, indicating necessity of supplementary feeding during dearth periods. Sugar syrup was the most economical and effective carbohydrate source for feeding A. mellifera colonies followed by liquid glucose. Pollen supplement feeding along with carbohydrate syrups significantly improved survival and growth of A. mellifera colonies during dearth period. HFRS was comparatively less effective carbohydrate source when fed alone however; with pollen its performance improved significantly. Jaggery was the least preferred source and not at all recommended for dearth feeding, as it induced maximum mortality (27.0 to 27.7 dead bees/feeder/week), fermented faster and attracted intruders like wasps. Incidence of insect-pests, mites and diseases was lowered by proper hygiene, care and supplementary feeding during dearth periods. Inter and intra-specific robbing was characteristic feature during dearth period in A. mellifera colonies. Most of the honey quality parameters although remained within the prescribed limits but variations were recorded in HMF (3.33-26.16 mg/kg), moisture (15.87- 20.10 g/100g), F:G ratio (1.00-1.09), ash content (0.17-0.45 g/100g) and acidity (0.042-0.062 g/100g), while sucrose remained below LOQ, i.e. 0.5g/100g. Organoleptic scores revealed maximum acceptability of fresh honey (8.43-8.60) while honeys from HFRS fed colonies had the least acceptance (7.03-7.40). Seventy one different plant species belonging to 31 families were foraged by bees during dearth period. Protein content and calorific value in different pollen types ranged from 15.5-42.5% and 399.6-493.0 Kcal/100g, respectively.
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    ThesisItemOpen Access
    Epidemiology, induction of systemic resistance and management of charcoal rot of sesame incited by Macrophomina phaseolina (Tassi) Goid.
    (CCSHAU, Hisar, 2020-01) Gupta, Ramesh Nath; Saharan, Hawa Singh
    Charcoal rot disease incited by Macrophomina phaseolina (Tassi) Goid. is the most destructive disease of sesame and causes heavy losses. The disease appears moderate to severe form every year in Haryana. Keeping in view of economic importance of this disease, the present study on epidemiology, induction of systemic resistance and management has been conducted under laboratory, screen house and field conditions during kharif 2017 and 2018 at CCS HAU, Hisar. Epidemiological studies showed the onset of charcoal rot varied in varieties HT 1 and HT 2 during different dates of sowing. The disease first appeared in early sown crop with higher disease incidence; however, disease appearance was delayed in mid and late sown with lower incidence of disease. The intensity of disease in mid sown and late sown was almost similar however; rate of disease progression was higher in late sown as compared to mid sown conditions. In vitro evaluation of non-conventional chemicals, salicylic acid (SA) was found most effective in inhibiting mycelial growth of M. phaseolina followed by IAA and IBA. Under screen house conditions, SA showed maximum reduction of charcoal rot incidence than other chemicals. Similarly, under field conditions, SA enhanced the yield and yield attributing traits than other treatments. Induction of total phenol content, total sugar content, peroxidase (POX), polyphenol oxidase (PPOX), phenylalanine ammonia lyase (PAL) and catalase activity was significantly increased by the treatment of SA followed by IAA, IBA and least by riboflavin. These activities reached at peak on six days after inoculation of pathogen and afterwards declined in both the varieties. However, variety HT 2 showed higher induction of biochemical activities than HT 1. Phytoextracts were evaluated for control of disease under in vitro conditions in which garlic extract (10%) showed maximum inhibition of mycelial growth followed by onion, karanj, neem and least by lantana extract. Similarly, under screen house conditions, garlic extract more reduced the incidence of charcoal rot than other phytoextracts. Under field conditions, seed treatment with combination of fungicides (carboxin+thiram) was most effective in controlling charcoal rot disease as well as enhanced the yield and yield attributing traits. Three hundred fifty sesame germplasm lines were evaluated for their relative resistance under field conditions in which majority of them showed moderately susceptible and susceptible reaction against charcoal rot. None of them identified as resistant, whereas only eighteen lines showed moderately resistant reaction against charcoal rotdisease
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    ThesisItemOpen Access
    Studies on the population dynamics, monitoring, mortality and control of Bemisia tabaci (Genn.) on cotton crop
    (CCSHAU, Hisar, 2020-07) Mehra, Swati; Rolania, Krishna
    The present investigation entitled “Studies on the population dynamics, monitoring, mortality and control of Bemisia tabaci (Genn.) on cotton crop” was carried out during 2017 and 2019, both in the Laboratory as well as at Experimental Area, Department of Entomology, CCS Haryana Agricultural University, Hisar. The population dynamics study revealed that peak activity of whitefly and its parasitoids, Encarsia spp. was observed from April to May on brinjal, cucumber and okra; in August on greengram, soybean and okra; and in September on cotton. In descending order, the peak abundance of whitefly was observed on cotton followed by cucumber, soybean, greengram, brinjal and okra while, parasitization by Encarsia spp. was highest on soybean followed by cotton, greengram, brinjal, cucumber and okra during 2017 and 2019. A total of 46 host plants belonging to 12 families were reported to be infested by whitefly, B. tabaci. Monitoring studies revealed that peak population of whitefly adults on yellow sticky traps (14,392 whitefly adults/trap) and in direct count (19.06 whitefly adults/leaf) was recorded during 38th SMW i.e., 3rd week of September; whereas, whitefly nymphal population i.e., 31.2 per leaf by direct count method was at its peak during 39th SMW i.e., 4th week of September. In direct count, whitefly adult population showed a significant negative correlation with evening relative humidity (r = -0.53) (r = -0.56) and total rainfall (r = -0.61) (r = -0.58) during 2017 and 2019, respectively. Nymphal population showed significant negative correlation with evening relative humidity (r = - 0.52) and total rainfall (r =-0.54) in 2019. Population captured on yellow sticky traps showed no correlation with weather parameters in 2017. While, whitefly adults captured on both horizontal (r = 0.54) (r = -0.51) and vertical traps (r = 0.55) (r = -0.51) showed significant positive and negative correlation with sunshine hours and total rainfall, respectively in 2019. Across all the immature developmental stages, principal mortality governing factor was dislodgement in cotton, predation in brinjal and parasitism in cucumber crop. The highest mortality occurred during fourth nymphal instar on cotton, brinjal and cucumber. Azadirachtin 0.03 per cent @ 7.5 ml/l was found more effective against whitefly adults and nymphs. However, it remained significantly at par with Azadirachtin 0.03 per cent @ 5 ml/l (recommended dose) and Azadirachtin 0.15 per cent at the dose of 7.5 ml/l and 5 ml/l.
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    ThesisItemOpen Access
    Studies on biodiversity and DNA barcoding of predatory ladybird beetles (Coleoptera: Coccinellidae) in Haryana
    (CCSHAU, Hisar, 2020-08) Mohammad Thamseer M K; Yadav, S.S
    The biodiversity of predatory ladybird beetles was investigated in the state of Haryana,India. The predators were collected from natural and manipulated ecosystems across the state by hand collection. The collected specimen were identified with appropriate taxonomic keys. The state presented a highly abundant but less diverse ecosystem with respect to coccinellid predators. 2253 specimen of 20 species belonging to coccinellidae were collected from 17 ecosystems. The Shannon-weiner index,Simpson’s index,Effective number of species,Margalef’s index and Pielou’s evenness index were calculated for different ecosystems and it was found that sheesham (Dalbergia sisoo) and Okra (Abelmoschus esculentus) was ecologically the most diverse ecosystem in the state. The diversity indices of the predators in the state was very low for most of the crop ecosystems which can be attributed to the fact that the state is one of the leading consumer of insecticides in the country. Thus, the present data reflects on the grave consequences of indescriminate usage of pesticides on the natural enemies which, if left unchecked, can cause potential localised extinction of predatory fauna. DNA of the six species among the collection were successfully barcoded and the phylogenetic tree revealed a close relationship between Chilocorinae and Scymninae. The temporal distribution of the major aphidiphagous predators in wheat ecosystem revealed the presence of five major predators including two syrphids viz., Episyrphus viridaureus and Ischiodon scutellaris and three coccinellids viz., Hippodamia variegata, Cheilomenes sexmaculata and Coccinella septempunctata. The cumulative population of the predators had significant positive correlation with aphid population (r= 0.999).
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    ThesisItemOpen Access
    Determination of pollen trapping frequency, pollen collection and hoarding behaviour in Apis mellifera L. colonies
    (CCSHAU, Hisar, 2019-09) Dalal, Pradeep Kumar; Yadav, Sunita
    With a view to explore the potential of pollen load trapping as subsidiary occupation among Indian beekeepers, the present investigations were carried out in 3 seasons (mustard bloom at Hisar and Kaul; multi-floral season at Kaul) during 2016-17 and 2017-18 to determine the optimum pollen trapping frequency that allow economically viable pollen collection as well as sufficient honey production and colony development. Fifteen colonies each of high (12-frame) and medium strength (8-frame) were put to five pollen trapping frequencies viz. daily, alternate days, 3 days, weekly and without trapping (control). The floral biology of dominant flowering crop under different seasons revealed that each flower of mustard, Brassica campestris and Egyptian clover, Trifolium alexandrium offered floral resources to A. mellifera bees for a period of 3 and 3.9 days, respectively. Mean pollen collection from high and medium strength colonies was 14.9 and 8.1 g/colony/day, translating to 1.98 and 1.09 kg/colony, respectively in mustard season. Pollen collection increased with increasing order of trapping frequencies. Pollen collection from high strength colonies from mustard was more in daily pollen trapping (25.3 g/colony/day) followed by alternate (18.4 g), 3 days (17.1) and weekly trapped colonies (14.0 g/colony/day). Similar trend was noticed for medium strength colonies on mustard bloom and multi-floral season, indicating direct relationship of pollen trapping with pollen load collection. Botanical diversity of pollen loads was estimated by employing 3 methods viz. visual, segregation on colour basis and haemocytometer method. Melissopalynological analysis identified 11 pollen types, yellow coloured B. campestris pollen being major source both at Hisar and Kaul comprising >50% of total pollen loads. During multi-floral season (Mar-Apr), white coloured pollen loads from weed, Ageratum conyzoides were in majority during initial phase and T. alexandrium in final phase. Crude protein and calorific value of B. campestris was 24.3% and 367.1 Kcal/100 g while values for A. conyzoides and T. alexandrium were 16.0 and 23.8% and 422.6 and 418.9 Kcal/100 g, respectively. Impact of different pollen trapping frequencies was studied on colony traits viz. honey, nectar, and pollen store, brood area, drone numbers, etc. During mustard bloom, in daily trapping the honey area depleted in high strength colonies (-5.0%) while in medium strength grew by 43.0%. Alternate trapping of high strength and 3 days trapping of medium strength colonies allowed honey area to expand by 95.3 and 73.1%, respectively over season. During multi-floral season, honey area dipped uniformly irrespective of trapping frequencies. Brood development suffered as a result of daily pollen trapping during mustard bloom as high and medium strength expanded by -2.9 and 11.0%. Under alternate trapping of high and 3 days trapping medium strength colony the brood area grew by 22.5 and 49.6%, respectively. During multi-floral season, shortage of good quality major floral source, led to reduction in total brood area across all trapping frequencies. Daily trapping of high strength colonies led to depletion of pollen store area (-24.7%) in colonies which triggered them to additionally recruit maximum proportion of foragers (29.0%) for pollen collection from existing foraging force, impacting other colony functions. Similarly medium strength daily trapped colony recruited additional pollen foraging force of 25.6%. Experimental colonies were also categorized based on pollen hoarding behavior and their sensitivity to hygiene. A total of 14 colonies possessed high pollen hoarding behaviour out of 90 colonies tested during all the season. Moderately sensitive (MS) trait alongside high pollen hoarding character was found in only one of the total lot of colonies. Overall analysis including economics suggested alternate trapping of high strength colonies as more remunerative in comparison to other trapping frequencies with adequate pollen yield, optimum colony growth and honey production with additional economic returns of Rs 264.9 per colony. Among medium strength colonies, 3 days pollen trapping was relatively better with net income of Rs 4.9 as per colony in addition to prolific honey production and brood development.
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    ThesisItemOpen Access
    Studies on molecular variability in whitefly, Bemisia tabaci (Gennadius) population in cotton and its management
    (CCSHAU, Hisar, 2019-06) Roomi Devi; Yogesh Kumar
    The present investigation was carried out at CCS Haryana Agricultural University, Hisar during Kharif seasons of 2017 and 2018. A survey of cotton growing areas was done in north region of India viz. Haryana, Punjab and Rajasthan and whitefly samples were collected to find out the molecular variation among whitefly. MtCOI gene specific primers were used to amplify the mtCOI gene segment of extracted DNA of whitefly. Screening of forty cotton genotypes were done against whitefly and level of resistance was further correlated with different biochemical and morphological traits. Different crops like sorghum, bajra, pigeon pea, cowpea and sesame were grown as intercrops with cotton to know their effects on whitefly population. Efficacy of different pesticides were tested against whitefly. For this two spray were done at ETL and data was recorded before spray and 1st, 3rd, 7th and 10th DAS. Results revealed that in all the samples mtCOI gene segment were amplified at 1000bp band. Sequences were generated from these samples and aligned along with reference sequences all over the world representing seven different clades of B.tabaci to analyse phylogenetic relationship. It was found that our sequences belong to Asia II-1 and clustered in clade of representing Asia II-1 biotype from Northern region of India. During both the years population of whitefly commenced in month of June (24th SMW) and continued till harvest of crop (41st SMW) with two peaks. Observations during Kharif 2017, 2018 and pooled analysis of both the years showed that maximum population was recorded in ARBH 1601 (15.81, 9.76, 12.79 adults/leaf) and minimum in HS 286 (10.03, 5.21, 7.62 adults/leaf), respectively. Whitefly population showed significant negative correlation with maximum temperature and significant positive with morning relative humidity. Population of whitefly showed significant and positive correlation with total sugar content, trichomes density while non significant and positive with protein and chlorophyll content. Significant and negative correlation with phenol, tannin and leaf thickness. Intercropping system revealed significant suppression in all the intercropped treatments over the sole cotton crop. Pooled mean of both the years revealed that minimum population was recorded in cotton intercropped sorghum (3.69 adults/leaf) and maximum in sole cotton (10.82 adults/leaf). Pooled mean of both the years 2017 and 2018 showed that the flonicamid 50% WG @ 150 g/ha (68.65%) was the most effective insecticide while Verticilium lecanii 1.15WP @ 2.5kg/ha (41.11%) was least effective.
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    ThesisItemOpen Access
    Incidence of major insect-pests, genotypes evaluation and management of pod borer complex in pigeonpea
    (CCSHAU, Hisar, 2020) Kanchan; Verma, Tarun
    Experiment on “Incidence of major insect-pests, genotypes evaluation and management of pod borer complex in pigeonpea” was conducted during kharif, 2019. Four pigeonpea genotypes (PAU 881, AL 1747, Paras and UPAS 120) were sown to study incidence of Helicoverpa armigera, Maruca vitrata, Clavigralla gibbosa and Empoasca kerri. Incidence of H. armigera commenced in 37th Standard Meteorological Week (SMW), remained upto 48th SMW, reached it’s peak in 44th SMW and population ranged from 0.05 to 0.45 larvae per plant. Web formation by M. vitrata and larvae started to appear from 37th SMW and persisted upto 43rd SMW. Peak of M. vitrata larvae was in 39th SMW (in Paras) and 40th SMW (AL 1747, UPAS 120 and PAU 881). Number of webs, larvae of M. vitrata ranged from 0.94 to1.23 webs per plant and 0.05-0.90 larvae per plant, respectively. Clavigralla gibbosa (Nymph and adult) incidence started from 36th SMW and continued upto 47th SMW. Adults of C. gibbosa attained peak in 40th SMW in all genotypes (in AL 1747: 39th SMW). Population varied from 0.08-0.20 nymph/plant and 0.20-3.69 adult/plant during period of study. M. obtusa persisted from 41st SMW to 48th SMW and reached it’s peak in 45th SMW. Empoasca kerri nymphs persisted from 29th SMW to 43rd SMW (AL 1747: 44th SMW) with peak in 33rd SMW. Pod damage (%) by M. vitrata, H. armigera, C. gibbosa and M. obtusa was reported as 2.10, 4.03, 25.16 and 2.31%, respectively. Grain yield of different genotypes ranged from 775-1200 kg/ha. Significant and negative correlation was found between H. armigera and minimum temperature, evening relative humidity, average relative humidity, wind speed and rainfall. Nymphal population of C. gibbosa exhibited significant and negative correlation with temperature, wind speed, while adults showed significant and negative correlation with relative humidity (Eve. and Avg.). E. kerri population exhibited significant and positive correlation with temperature (minimum and maximum), however, M. obtusa population showed significant and negative correlation with temperature, relative humidity (avg. and eve.) and wind speed. Genotypes evaluation studies showed minimum pod damage in AH 9-18 (for M. vitrata, H. armigera and M. obtusa), AH 16-02, B-17200, H 00-15 (for H. armigera). Grain yield ranged from 777.78 kg/ha (Paras) to 1859.26 kg/ha (AH 9-18). Evaluation of insecticides against pod borer complex indicated spinosad 45 SC as most effective against M. vitrata, H. armigera and M. obtusa. Spinosad 45 SC was found at par with indoxacarb 14.5 SC (M. vitrata and H. armigera), chlorantraniliprole 9.3 + lambda-cyhalothrin 4.6% ZC, lambda-cyhalothrin 5 EC, emamectin benzoate 5 SG and deltamtherin 2.8 EC (H. armigera) and azadirachtin 0.03% (M. obtusa). Maximum grain yield was obtained in spinosad 45 SC (919.26 kg/ha) sprayed plot.
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    ThesisItemOpen Access
    Studies on insecticide use pattern, population susceptibility and insecticide evaluation against Amrasca biguttula biguttula (Ishida) in cotton
    (CCSHAU, Hisar, 2020-08) Pritam Kumari; Anil
    A survey was carried out to study the insecticide use pattern in Sirsa, Hisar and Bhiwani districts of Haryana during kharif, 2019. The maximum respondents (70.00%) were found to be belonging to the age group of 40-60 years. Education status of farmers was quite good with an overall literacy rate of 87.00% and 56.00% of the respondents were having more than 15 years of experience in cotton cultivation. Most commonly grown Bt cotton hybrid by the majority of farmers (34%) was RCH 773 BG II followed by RCH 776 BG II (31.30%), US 51 BG II (13.30%), Ankur 3028 BG II (13.30%) and US 81 BG II (8.00%). Based on farmers experience, whitefly, Bemisia tabaci and leafhopper, Amrasca biguttula biguttula were the most important pests among sucking pests in Bt cotton. All the farmers were found to target whitefly and leafhopper for control whereas in case of dusky cotton and red cotton bug none was reported to targeting for control. The incidence, severity and yield losses of sucking pests especially whitefly and leafhopper were estimated to be high by 100, 86.67 & 82.70; 76.67, 74.00 & 64.67% farmers, respectively. The cent per cent farmers was found to adopt control measures at 61-90 days after sowing as highest pest incidence was estimated by cent per cent farmers at this duration. The majority of the farmers (44.67%) were found to be dependant on Agriculture input dealers for information in cotton cultivation followed by CCS HAU Hisar/CICR-RS (34.66%). Major constraints faced by farmers in insect pest management were high cost of pesticides, weather problems and lack of knowledge about bioagent and insubstantial control or poor efficacy of insecticides. Insecticides like imidacloprid, thiamethoxam, emamectin benzoate, fipronil, dimethoate and monocrotophos were mainly used by farmers to control sucking pests. Besides, newer insecticides like flonicamid, dinotefuran, diafenthiuron, spiromesifen and spinetoram were also being used by farmers. Insecticide-bioassays were carried out to assess the susceptibility of field-collected cotton leafhopper from Hisar, Sirsa and Bhiwani districts to commercial formulations of imidacloprid 17.8% SL, thiamethoxam 25% WG and flonicamid 50% WG during kharif, 2019. Intrinsic toxicity data on imidacloprid 17.8% SL, thiamethoxam 25% WG and flonicamid 50% WG indicated that Bhiwani population was most susceptible to these insecticides with LC50 values ranging from 7.23-24.74 ppm followed by Hisar population (10.09-29.83 ppm) whereas Sirsa population was found to be least susceptible with LC50 values of 12.17-34.60 ppm. Among different insecticides, flonicamid 50% WG with LC50 values ranging from 7.23 to 12.17 ppm showed maximum toxicity followed by thiamethoxam 25% WG 20.73 to 29.60 ppm while imidacloprid 17.8% SL was found to be least toxic insecticide with LC50 ranging from 24.74 to 34.60 ppm. Based on mean of two sprays in a field trial during kharif, 2019, the lowest mean nymhal and adults population (No. per 3 leaves) were recorded in flonicamid 50% WG @ 150 g ha-1 (0.84 nymphs and 1.73 adults) followed by dinotefuran 20% SG @ 150 g ha-1 (1.49 nymphs and 2.06 adults) and diafenthiuron 50% WP @ 500 g ha-1 (1.65 nymphs and 2.25 adults). Imidacloprid 17.8% SL @ 100 ml ha-1, thiacloprid 21.7% SC @ 125 ml ha-1, thiamethoxam 25% WG @ 100 g ha-1 and buprofezin 25% SC @ 1000 ml ha-1 were found to be moderately effective insecticides. Monocrotophos 36% SL @ 437 ml ha-1 was found to be the least effective insecticide. The non-significant differences were observed in the population of natural enemies (spiders, coccinellids & Chysoperla) among the different treatments. The highest seed cotton yield (q ha-1) was obtained with flonicamid (21.60), followed by dinotefuran (20.99), diafenthiuron (20.71), imidacloprid (20.41), thiacloprid (20.12), buprofezin (19.87), thiamethoxam (19.83) and monocrotophos (19.37). On the basis of cost of protection, the incremental cost benefit ratio was maximum with imidacloprid (1:6.36) followed by thiacloprid (1:5.64), thiamethoxam (1:5.29), monocrotophos (1:4.74), dinotefuran (1:4.38), flonicamid (1:3.99) and buprofezin (1:3.86).