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Chaudhary Charan Singh Haryana Agricultural University, Hisar

Chaudhary Charan Singh Haryana Agricultural University popularly known as HAU, is one of Asia's biggest agricultural universities, located at Hisar in the Indian state of Haryana. It is named after India's seventh Prime Minister, Chaudhary Charan Singh. It is a leader in agricultural research in India and contributed significantly to Green Revolution and White Revolution in India in the 1960s and 70s. It has a very large campus and has several research centres throughout the state. It won the Indian Council of Agricultural Research's Award for the Best Institute in 1997. HAU was initially a campus of Punjab Agricultural University, Ludhiana. After the formation of Haryana in 1966, it became an autonomous institution on February 2, 1970 through a Presidential Ordinance, later ratified as Haryana and Punjab Agricultural Universities Act, 1970, passed by the Lok Sabha on March 29, 1970. A. L. Fletcher, the first Vice-Chancellor of the university, was instrumental in its initial growth.

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Subject: Botany × Author: Nisha × End date: 2015 × Start date: 2010 × Thesis Type: M.Sc ×
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    ThesisItemOpen Access
    Effect of salinity on morphophysiological characteristics of Dalbergia sissoo Roxb.
    (CCSHAU, 2015) Nisha; Varshney, U.K.
    The objectives of present investigation were to observe the effect of chloride-dominated salinity on morpho-physiological characteristics of Dalbergia sissoo saplings and to study the protein profile by SDS-PAGE in control versus saline treated leaf. Two experiments were conducted in the screen house on one year old Dalbergia sissoo saplings subjected to nutrient supplemented chloride dominated salinity treatments at 4 EC level viz., 0 (control), 4, 8, and 12dSm-1. The first experiment was conducted with an objective to study the effect of chloride-dominated salinity on some morphophysiological characteristics of Dalbergia sissoo saplings. The study revealed the deleterious effects of salinity on plant height, stem, leaf and root dry mass, root length and root volume. All these parameters suffered a significant reduction with progressive increase of EC level in the growth medium. Chlorophyll content, starch and total leaf protein, RWC significantly reduced while accumulation of total soluble solids, total soluble sugar and proline in leaves was noticed with the rise of salinity in the growth medium. Membrane injury index of leaves drastically increased at 8 and 12dSm-1 EC levels as compared to the control due to membrane injury caused by toxic level of ions. Significant accumulation Na+, Cl- and SO4 2- and decline of K+ contents in leaves was quiet evident. In the second experiment protein profile by SDS-PAGE in control vs. saline treated plant leaves at the termination of experiment was studied. The study showed variation in the protein profile of leaves at different EC levels. Under 4dSm-1 EC level of salinity 3 new protein bands of molecular weight 12.5, 24.0 and 47.8 kDa were synthesized de novo and one protein band of 23.7 kDa was disappeared while under 8dSm-1 EC level, two new protein bands of molecular weight 16.3 and 17.0 kDa were de novo synthesis and one protein band 18.4 was disappeared. At 12dSm-1 EC level of salinity 2 new protein bands 21.2 and 28.1 kDa molecular weight were synthesized de novo while one protein band of molecular weight 28.6 disappeared. Salinity in general was found deleterious to Dalbergia sissoo saplings as there was a retarding effect on almost all the morphological, physiological and biochemical parameters studied. The saplings although survived at the highest level of salinity studied (12dSm-1) by osmotic adjustment and synthesis of salt induced proteins but an overall diminished growth indicated the salt sensitivity of the tree species.