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Chaudhary Charan Singh Haryana Agricultural University, Hisar

Chaudhary Charan Singh Haryana Agricultural University popularly known as HAU, is one of Asia's biggest agricultural universities, located at Hisar in the Indian state of Haryana. It is named after India's seventh Prime Minister, Chaudhary Charan Singh. It is a leader in agricultural research in India and contributed significantly to Green Revolution and White Revolution in India in the 1960s and 70s. It has a very large campus and has several research centres throughout the state. It won the Indian Council of Agricultural Research's Award for the Best Institute in 1997. HAU was initially a campus of Punjab Agricultural University, Ludhiana. After the formation of Haryana in 1966, it became an autonomous institution on February 2, 1970 through a Presidential Ordinance, later ratified as Haryana and Punjab Agricultural Universities Act, 1970, passed by the Lok Sabha on March 29, 1970. A. L. Fletcher, the first Vice-Chancellor of the university, was instrumental in its initial growth.

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Subject: Biotechnology and Molecular Biology × Author: Sunil Kumar × End date: 2009 ×
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    ThesisItemOpen Access
    Studies on myb gene expression in Indian mustard (Brassica juncea L. Coss. & Czern) under salt stress
    (CCSHAU, 2007) Sunil Kumar; Yadav, R.C.
    The investigation was carried out to study the myb expression under different levels of salinity in Brassica juncea cv. CS52 in the Department of Biotechnology & Molecular Biology, CCS Haryana Agricultural, Hisar during the year 2006 and 2007. The myb gene expression was checked by RT-PCR approach. Primers BjMYBR-1 and BjMYBR-2 were designed from the conserved DNA binding domain of Atmyb2 by analyzing this gene sequence with other myb gene sequence available in database using ClustalW programme. The expression of Bjmyb1 became detectable within 15 minutes of salinity exposure, which increased and was maximum in 30 minutes of salinity treatment. Thereafter it declined and stabilized in 2 hr of salinity treatment. The expression was not affected by the different concentrations (100 mM, 200 mM, 300 mM and 400 mM) of NaCl used. Sequencing of amplified cDNA fragment revealed a 176 bp sequence. ClustalW analysis of Bjmyb1 with other eight myb genes from different organisms available in database showed its homology with Atmyb2 and Chmyb2.