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Chaudhary Charan Singh Haryana Agricultural University, Hisar

Chaudhary Charan Singh Haryana Agricultural University popularly known as HAU, is one of Asia's biggest agricultural universities, located at Hisar in the Indian state of Haryana. It is named after India's seventh Prime Minister, Chaudhary Charan Singh. It is a leader in agricultural research in India and contributed significantly to Green Revolution and White Revolution in India in the 1960s and 70s. It has a very large campus and has several research centres throughout the state. It won the Indian Council of Agricultural Research's Award for the Best Institute in 1997. HAU was initially a campus of Punjab Agricultural University, Ludhiana. After the formation of Haryana in 1966, it became an autonomous institution on February 2, 1970 through a Presidential Ordinance, later ratified as Haryana and Punjab Agricultural Universities Act, 1970, passed by the Lok Sabha on March 29, 1970. A. L. Fletcher, the first Vice-Chancellor of the university, was instrumental in its initial growth.

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  • ThesisItemOpen Access
    In-silico identification of miRNA in Rice (Oryza sativa L.)
    (CCSHAU,HiSAR, 2020) Bhavya; Sudhir Kumar
    Rice (Oryza sativa L.) is the world’s single most important and essential crop and a primary food source for half of the world’s populace. An aggregate of 49% calories consumed by the human population come from rice. Micro RNAs (miRNA) are single-stranded non coding RNA molecules of about 21-23 nucleotides in length which regulate gene expression. The main function of miRNAs is to down-regulate the gene expression. The twelve chromosomes of Oryza sativa was downloaded from the International Rice Genome Sequencing Project (IRGSP), after that they transcribed into RNA and then they were cut into fragments by using split command. The output of split command was subjected as input for palindrome finding in palindrome.pl script. Only four mismatches were allowed. The secondary structures and MFE of output of palindrome.pl script were predicted using RNAfold program. The sequences whose energy were less than -40kcal were sorted and identified as putative miRNA. These putative miRNA were compared against miRNA database in miRBase. Total 61 miRNAs and their 255 putative targets were identified. These target genes have regulatory functions in some certain biological processes.