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Chaudhary Charan Singh Haryana Agricultural University, Hisar

Chaudhary Charan Singh Haryana Agricultural University popularly known as HAU, is one of Asia's biggest agricultural universities, located at Hisar in the Indian state of Haryana. It is named after India's seventh Prime Minister, Chaudhary Charan Singh. It is a leader in agricultural research in India and contributed significantly to Green Revolution and White Revolution in India in the 1960s and 70s. It has a very large campus and has several research centres throughout the state. It won the Indian Council of Agricultural Research's Award for the Best Institute in 1997. HAU was initially a campus of Punjab Agricultural University, Ludhiana. After the formation of Haryana in 1966, it became an autonomous institution on February 2, 1970 through a Presidential Ordinance, later ratified as Haryana and Punjab Agricultural Universities Act, 1970, passed by the Lok Sabha on March 29, 1970. A. L. Fletcher, the first Vice-Chancellor of the university, was instrumental in its initial growth.

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2012 - 20133
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Subject: Biochemistry × End date: 2014 × Start date: 2012 × Type: Thesis × Thesis Type: Ph.D ×
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    ThesisItemOpen Access
    Effect of drought stress on starch metabolism in late sown wheat (Triticum aestivum L.) during grain development
    (CCSHAU, 2013) Mahla, Reena; Madan, Shashi
    The present investigation was conducted to investigate the effect of drought stress on starch metabolism in late sown wheat (Triticum aestivum L.) during grain development. The four genotypes i.e. two tolerant (WH 1021 and WH 1080) and two susceptible (WH 711 and HD 2687) were raised in late sown conditions under irrigated and drought stress conditions. Drought stress was created by withholding the irrigation at anthesis stage. Analysis of data reavealed that during grain development, starch metabolising enzymes i.e. sucrose synthase, ADP-glucose pyrophosphorylase, soluble starch synthase, starch branching and starch debranching enzyme increased in all genotypes and reached their peaks but their maximum peak values and time at which they reached were different in different genotypes. Increase in sugar content was found under drought stress conditions in comparison to irrigated conditions at different days after anthesis. Water stress caused a marked reduction in starch content of grains. In all genotypes level of ADP-glucose and UDP-glucose increased up to 21st DAA and then declined. Physiological parameters like relative water content, osmotic potential, chlorophyll content, chlorophyll fluorescence, cell membrane thermostability and canopy temperature depression declined under drought stress conditions over irrigated conditions. Quality parameters like moisture content and crude fibre decreased whereas, sedimentation value, gluten, protein content and grain hardness increased under drought stress condition over irrigated conditions. Yield parameters like grain number per spike, grain weight, number of productive tillers, biomass per plot and grain yield decreased under drought stress. The reduction in physiological, biochemical, yield and grain quality traits was found to more pronounced in the susceptible genotypes in comparison to the tolerant ones. WH 1021 was adjudged to be most tolerant genotype.
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    ThesisItemOpen Access
    Biochemical studies on amelioration of phytotoxic effects of chromium (VI) in clusterbean [Cyamopsis tetragonoloba (L.) Taub.]
    (CCSHAU, 2013) Punesh; Joshi, U.N.
    Heavy metals are the intrinsic component of the environment with both essential and non essential types. Their excessive levels pose a threat to plant growth and yield. Also, some heavy metals are toxic to plants even at very low concentrations. The present investigation had been done to study the effect of amelioration of toxic effect of Cr (VI) on morphological and biochemical parameters of clusterbean plants. The crop was raised in pots filled with sandy loam soil in naturally lit net house. The soil in each pot treated with 0.0, 0.5, 1.0, 2.0 and 4.0 mg Cr (VI) kg-1 soil. Morphological parameters like plant height, number of leaves, root length, fresh and dry weight of leaves, stem and root decreased with an increase in the chromium concentration. Growth of clusterbean plants was reduced very significantly in presence of 2 mg Cr (VI) kg-1 soil. The chlorophyll ‘a’, chlorophyll ‘b’, total chlorophyll, carotenoids content, non-structural carbohydrates, crude protein content and IVDMD decreased, whereas, structural carbohydrates viz. NDF, ADF, cellulose, lignin and silica significantly increased with increasing chromium concentration. Minerals like sodium, chromium content increased with increase in supply of chromium concentration. The iron, zinc and potassium content decreased with increase in chromium concentration. Chromium treatment adversely affect activity of enzymes of nitrogen metabolism viz. nitrogenase, NR, NiR, GS, GDH and GOGAT.To ameliorate toxic effect of Cr (VI), different sets of plants were treated with salicylic acid and ferrous sulphate–EDTA solution through different modes viz. pre-treatment, soil treatment and foliar spray or both. Loss in growth, biochemical and nutritional parameters of clusterbean plants was recovered to different extent by different treatments with salicylic acid (0.25 and 0.5 mM) and FeSO4-EDTA solution (0.25 mM and 0.5 mM). The recovery in biomass of clusterbean plants was maximum with combined soil pretreatment and foliar application of 0.50 FeSO4-EDTA solution. In current study, salicylic acid application by different modes also emerged as a potential way to ameliorate Cr toxicity from economically important crops or plants.
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    ThesisItemOpen Access
    Studies on osmoprotective role of trehalose in thermotolerance of wheat (triticum aestivum L.)
    (CCSHAU, 2012) Yogita; Madan, Shashi
    The present investigation was carried out to study the osmoprotective role of trehalose in thermotolerance of wheat (Triticum aestivum L.). Two wheat genotypes viz. WH-730 (thermotolerant) and WH-711 (thermo-sensitive) were grown under control conditions in seed germinator at 25ºC. The 10 days old seedlings were exposed to 35ºC. These seedlings were supplied with Hogland solution containing 1mM and 2mM trehalose. All biochemical (enzymatic and non-enzymatic) and physiological parameters were studied in the leaves of control (25ºC), stressed (35ºC) and trehalose (1mM and 2mM) treated seedlings on 15th day of germination. Analysis of data revealed that activity of H2O2 scavenging enzymes such as CAT, POX and APX increased under heat stress in both the genotypes while decreased with the increasing concentration of trehalose from 1mM to 2mM. The LOX activity, MDA and H2O2 content increased under heat stress conditions when compared with controls reflecting the higher lipid peroxidation and free radical production under stressed conditions whereas trehalose helped to recover from damage due to lipid peroxidation and free radical production. The metabolites like ascorbic acid and trehalose were increased under stressed conditions. Upon trehalose treatment, reduction in ascorbic acid content was observed while trehalose content further increased under trehalose treatment. The glucose content reduced under heat stress, but increased in treated seedlings. Physiological parameters like relative water content, osmotic potential, membrane thermo-stability and ratio of chlorophyll a and b were found to be higher in trehalose treated seedlings in comparison to stressed conditions. Excised leaf water loss capacity (ELWL) increased under stress conditions whereas trehalose treatment decreased ELWL.