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Chaudhary Charan Singh Haryana Agricultural University, Hisar

Chaudhary Charan Singh Haryana Agricultural University popularly known as HAU, is one of Asia's biggest agricultural universities, located at Hisar in the Indian state of Haryana. It is named after India's seventh Prime Minister, Chaudhary Charan Singh. It is a leader in agricultural research in India and contributed significantly to Green Revolution and White Revolution in India in the 1960s and 70s. It has a very large campus and has several research centres throughout the state. It won the Indian Council of Agricultural Research's Award for the Best Institute in 1997. HAU was initially a campus of Punjab Agricultural University, Ludhiana. After the formation of Haryana in 1966, it became an autonomous institution on February 2, 1970 through a Presidential Ordinance, later ratified as Haryana and Punjab Agricultural Universities Act, 1970, passed by the Lok Sabha on March 29, 1970. A. L. Fletcher, the first Vice-Chancellor of the university, was instrumental in its initial growth.

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2010 - 201426
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Subject: Biochemistry × End date: 2014 × Start date: 2010 ×
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Now showing 1 - 9 of 26
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    ThesisItemOpen Access
    Use of molecular markers for varietal identification and assessing phylogenetic status of Basmati rice in genus Oryza sativa L.
    (CCSHAU, 2011) Jyoti; Jain, Sunita
    Molecular markers provide novel tools for varietal identification, diversity analysis and assessing phylogenetic relationships among various rice groups in genus Oryza. A set of 50 rice genotypes comprising of seven japonica rice varieties, six traditional Basmati, thirteen cross-bred Basmati and 24 indica rice varieties was investigated using 54 markers (28 on chromosome 8 and 26 on rest of the chromosomes); data obtained has been used to assess the phylogenetic status of Basmati rice in genus Oryza sativa L. A total of 217 alleles were detected, with an average of 4.01 alleles per locus. Number of alleles per locus (3.5 alleles) for markers on chromosome 8 was less than the mean value (4.58 alleles) based on 26 SSR markers on rest of eleven chromosomes of rice. Eleven of these alleles were unique, present in only one genotype. Null allele was observed at only one locus (RM408) in Super Basmati. The number and size of alleles and Polymorphism Information Content (PIC) values ranged between 2-7, 83-381 bp and 0.074-0.810, respectively. An average PIC of 0.55 per locus was obtained, which confirms that markers used in this study were highly informative. Genetic relationships generated using the whole genome marker data as well as allelic profile on chromosome 8 and rest of eleven chromosomes separately placed 50 rice genotypes in three distinct clusters. Seventeen of the nineteen Basmati rice varieties (except two cross-bred Basmati rice varieties, Sabarmati and Improved Sabarmati) formed a separate cluster quite distinct from the indica and japonica rice clusters. Interestingly, mean similarity indices obtained from chromosome 8 dataset placed Basmati rice cluster at equal distance from japonica and indica rice types. However, whole genome as well as rest of the genome marker datasets placed Basmati group closer to indica rice varieties than japonica. Chromosome 8 dataset showed a positive correlation (Mantel test, r = 0.631) with the rest of the genome dataset, indicating a higher level of similarity between the two. All the traditional and crossbred Basmati rice varieties and two aromatic japonica varieties shared the same BAD2 alleles at the aroma locus, indicated the distinctness of the aroma locus. While the present study gives support to the hypothesis that japonica genotypes may have contributed towards the evolution of chromosome 8 or a part of chromosome 8 in Basmati rice, it also indicate the active flow of genes from indica rices during the course of parallel evolution in northern Indian sub-continent. The study also demonstrates the distinctness of TB from other rice types (indica and japonica) and also provides a number of novel SSR markers which can be used to differentiate within/among the various rice groups at commercial level. It was observed that the amplified products differing in ≥12bp can be separated on 3% agarose gels for varietal identification in Basmati rice, which is faster, much cheaper and relatively simple method compared to the advanced molecular methods already available for the purpose.
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    ThesisItemOpen Access
    Biochemical And Molecular Marker Analysis Of CSR10 (SALT Tolerant,Indica) X HBC19 (Taraori Basmati) Derived Recombinant Inbred
    (Department Of Biochemistry College Of Basic Sciences And Humanities CCS Haryana Agricultural University : Hisar, 2010) Bhanker,Vinita.; Jain,Sunita.
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    ThesisItemOpen Access
    Biochemical And Molecular Charaterzation Of Jatropha Curcas For Frost Tolerance
    (College Of Basic Sciences And Humanities CCS Haryana Agricultural University : Hisar, 2011) Arora,Ruby.; Singal,H.R.
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    ThesisItemOpen Access
    Biochemical Changes In Ber (Ziziphus Mauritiana Lamk.) Fruits During Ripening, Post Ripening And Storage
    (Chaudhary Charan Singh Haryana Agricultural University; Hisar, 2010) Praduman; Malhotra, Sarla
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    ThesisItemOpen Access
    Effect of drought stress on starch metabolism in late sown wheat (Triticum aestivum L.) during grain development
    (CCSHAU, 2013) Mahla, Reena; Madan, Shashi
    The present investigation was conducted to investigate the effect of drought stress on starch metabolism in late sown wheat (Triticum aestivum L.) during grain development. The four genotypes i.e. two tolerant (WH 1021 and WH 1080) and two susceptible (WH 711 and HD 2687) were raised in late sown conditions under irrigated and drought stress conditions. Drought stress was created by withholding the irrigation at anthesis stage. Analysis of data reavealed that during grain development, starch metabolising enzymes i.e. sucrose synthase, ADP-glucose pyrophosphorylase, soluble starch synthase, starch branching and starch debranching enzyme increased in all genotypes and reached their peaks but their maximum peak values and time at which they reached were different in different genotypes. Increase in sugar content was found under drought stress conditions in comparison to irrigated conditions at different days after anthesis. Water stress caused a marked reduction in starch content of grains. In all genotypes level of ADP-glucose and UDP-glucose increased up to 21st DAA and then declined. Physiological parameters like relative water content, osmotic potential, chlorophyll content, chlorophyll fluorescence, cell membrane thermostability and canopy temperature depression declined under drought stress conditions over irrigated conditions. Quality parameters like moisture content and crude fibre decreased whereas, sedimentation value, gluten, protein content and grain hardness increased under drought stress condition over irrigated conditions. Yield parameters like grain number per spike, grain weight, number of productive tillers, biomass per plot and grain yield decreased under drought stress. The reduction in physiological, biochemical, yield and grain quality traits was found to more pronounced in the susceptible genotypes in comparison to the tolerant ones. WH 1021 was adjudged to be most tolerant genotype.
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    ThesisItemOpen Access
    Use Of Molecular Markers For Warietal Identification And Assessing Phylogenetic Status Of Basmati Rice In Genus Oryza Sativa L.
    (Department Of Biochemistry College Of Basic Sciences And Humanities CCS Haryana Agricultural University : Hisar, 2010) Jyoti; Jain,Sunita.
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    ThesisItemOpen Access
    Biochemical studies on amelioration of phytotoxic effects of chromium (VI) in clusterbean [Cyamopsis tetragonoloba (L.) Taub.]
    (CCSHAU, 2013) Punesh; Joshi, U.N.
    Heavy metals are the intrinsic component of the environment with both essential and non essential types. Their excessive levels pose a threat to plant growth and yield. Also, some heavy metals are toxic to plants even at very low concentrations. The present investigation had been done to study the effect of amelioration of toxic effect of Cr (VI) on morphological and biochemical parameters of clusterbean plants. The crop was raised in pots filled with sandy loam soil in naturally lit net house. The soil in each pot treated with 0.0, 0.5, 1.0, 2.0 and 4.0 mg Cr (VI) kg-1 soil. Morphological parameters like plant height, number of leaves, root length, fresh and dry weight of leaves, stem and root decreased with an increase in the chromium concentration. Growth of clusterbean plants was reduced very significantly in presence of 2 mg Cr (VI) kg-1 soil. The chlorophyll ‘a’, chlorophyll ‘b’, total chlorophyll, carotenoids content, non-structural carbohydrates, crude protein content and IVDMD decreased, whereas, structural carbohydrates viz. NDF, ADF, cellulose, lignin and silica significantly increased with increasing chromium concentration. Minerals like sodium, chromium content increased with increase in supply of chromium concentration. The iron, zinc and potassium content decreased with increase in chromium concentration. Chromium treatment adversely affect activity of enzymes of nitrogen metabolism viz. nitrogenase, NR, NiR, GS, GDH and GOGAT.To ameliorate toxic effect of Cr (VI), different sets of plants were treated with salicylic acid and ferrous sulphate–EDTA solution through different modes viz. pre-treatment, soil treatment and foliar spray or both. Loss in growth, biochemical and nutritional parameters of clusterbean plants was recovered to different extent by different treatments with salicylic acid (0.25 and 0.5 mM) and FeSO4-EDTA solution (0.25 mM and 0.5 mM). The recovery in biomass of clusterbean plants was maximum with combined soil pretreatment and foliar application of 0.50 FeSO4-EDTA solution. In current study, salicylic acid application by different modes also emerged as a potential way to ameliorate Cr toxicity from economically important crops or plants.
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    ThesisItemOpen Access
    Isolation, Purification, Characterization And Immobilization Of Microbial Amylase
    (Chaudhary Charan Singh Haryana Agricultural University; Hisar, 2010) Nisha Kumari; Jain, Veena
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    ThesisItemOpen Access
    Isolation, Purification, Characterization And Immobilization Of Rennin From Microbial Sources
    (Chaudhary Charan Singh Haryana Agricultural University; Hisar, 2010) Kumari, Rajesh; Malhotra, Sarla