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Govind Ballabh Pant University of Agriculture and Technology, Pantnagar

After independence, development of the rural sector was considered the primary concern of the Government of India. In 1949, with the appointment of the Radhakrishnan University Education Commission, imparting of agricultural education through the setting up of rural universities became the focal point. Later, in 1954 an Indo-American team led by Dr. K.R. Damle, the Vice-President of ICAR, was constituted that arrived at the idea of establishing a Rural University on the land-grant pattern of USA. As a consequence a contract between the Government of India, the Technical Cooperation Mission and some land-grant universities of USA, was signed to promote agricultural education in the country. The US universities included the universities of Tennessee, the Ohio State University, the Kansas State University, The University of Illinois, the Pennsylvania State University and the University of Missouri. The task of assisting Uttar Pradesh in establishing an agricultural university was assigned to the University of Illinois which signed a contract in 1959 to establish an agricultural University in the State. Dean, H.W. Hannah, of the University of Illinois prepared a blueprint for a Rural University to be set up at the Tarai State Farm in the district Nainital, UP. In the initial stage the University of Illinois also offered the services of its scientists and teachers. Thus, in 1960, the first agricultural university of India, UP Agricultural University, came into being by an Act of legislation, UP Act XI-V of 1958. The Act was later amended under UP Universities Re-enactment and Amendment Act 1972 and the University was rechristened as Govind Ballabh Pant University of Agriculture and Technology keeping in view the contributions of Pt. Govind Ballabh Pant, the then Chief Minister of UP. The University was dedicated to the Nation by the first Prime Minister of India Pt Jawaharlal Nehru on 17 November 1960. The G.B. Pant University is a symbol of successful partnership between India and the United States. The establishment of this university brought about a revolution in agricultural education, research and extension. It paved the way for setting up of 31 other agricultural universities in the country.

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  • ThesisItemOpen Access
    Comparative evaluation of anaesthetic effects of various combinations of acepromazine, butorphanol, dexmedetomidine and ketamine in glycopyrrolate premedicated dogs maintained with Sevoflurane
    (G.B. Pant University of Agriculture and Technology, Pantnagar, District Udham Singh Nagar, Uttarakhand. PIN - 263145, 2021-02) Kundu, Saikat Kumar; Kandpal, Manjul
    Eighteen adult dogs (requiring various clinical procedures) used in this study were divided randomly into three subgroups (A, B and C). All the dogs were subjected to the administration glycopyrrolate at the dose rate of 0.01 mg/kg intramuscularly followed by acepromazine given as pre anaesthetic at the dose rate of 0.04 mg/kg IM in group A, butorphanol at the dose rate of 0.4 mg/kg IV in group B and dexmedetomidine 10 μg/kg IV in group C. Ten minutes after premedication, all the animals of each group were induced with intravenous ketamine. The induction doses were found to be 7.62±0.09 mg/kg in group A, 7.12±0.09 mg/kg in group B and 6.69±0.08 mg/kg in the animals of group C respectively. Intubation was easy and free of any major complications in the animals of group A and group C, however in the animals of group B, they showed strong coughing reflex, chewing motion and strong tongue with-drawl during the time of intubation. Anaesthesia was maintained in all the groups of animals with sevoflurane. The minimum concentration of inhaled sevoflurane for maintenance of anaesthesia was noted in all the animals by the tail clamp method. The level of anaesthesia was determined by observing various clinical (Induction time, intubation score, duration of anaesthesia, muscle relaxation, pedal reflex, recovery time, complete recovery time, recovery score, required doses of different drugs and minimum concentration of inhaled sevoflurane, physiological (rectal temperature, heart rate, respiration rate, blood pressure, capillary refill time, haemoglobin oxygen saturation-SpO2, electrocardiography), haematological (haemoglobin, total erythrocyte count, erythrocyte sedimentation rate, total leucocyte count, differential leucocyte count, packed cell volume, and biochemical (serum glucose, total protein, serum urea nitrogen, serum creatinine, alanine amino transferase ,aspartate amino transferase and total bilirubin) parameters. The animals of group C showed quicker induction. All anaesthetic combinations tested in the study were able to provide adequate muscle relaxation but group C showed better result. The pedal reflex was totally lost after administration of the induction agents in all the groups. The recovery time, complete recovery time were highest in group B followed by group C and group A. The minimum concentration of inhaled sevoflurane for maintenance of anaesthesia were found to be (1.68 ± 0.47)% for group A, (2.00 ± 0.25)% for group B and (1.85 ± 0.54)% for group C. The rectal temperature, heart rate and respiration rate showed significant decrease at various time intervals. The systolic, diastolic and mean arterial pressures showed significant decrease in its values in the groups A but not in the group B and group C, and returned to their base values by 90 minutes post anaesthesia. In all the groups of animals the capillary refill time (CRT) and haemoglobin oxygen saturation (SpO2) were always less than 2 seconds and more than 95% respectively. The haemoglobin, PCV, TLC and TEC values of the animals in various groups showed significant decrease in its values whereas the ESR showed a significant increase. Significant changes were observed in differential leucocyte count. Serum glucose, Serum urea nitrogen, creatinine, ALT, AST and total bilirubin levels increased significantly in all the groups of animals however there was significant decrease in total protein levels. On the basis of above mentioned parameters, it was concluded that the most efficacious anaesthetic combination was glycopyrrolate (0.01 mg/kg IM) dexmedetomidine (10 μg/kg IV)- ketamine (6.69±0.08 mg/kg IV) and sevoflurane (1.85 ± 0.54) % in the animals of group C and it was found suitable for surgeries because of its smooth induction, less induction time, excellent muscle relaxation and analgesia properties, maximum dose sparing effect on ketamine and smooth and uneventful recovery.
  • ThesisItemOpen Access
    Performance analysis of farmer producer organisations of Uttarakhand
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2021-11) Sandeep Kaur; Kameswari, V.L.V.
    Major proportion of India’s farmers are small and marginal farmers. They together, account for a whopping ownership of 85 percent of the total farm holdings in India. About 1.5 to 2.0 million new marginal and small farms are being added every year due to continued land fragmentation. Small and marginal farmers in India are highly vulnerable to risks and face multifold problems. A variety of approaches have emerged in response to the problems faced by them. Collectivization of small and marginal farmers into Producer Organizations (PO) is one of the most effective pathways to address the challenges faced by them as POs improve access to investments, technology and inputs and markets. Various efforts are being made by the government to promote FPOs. Despite these efforts, FPOs in many places are struggling to survive. Further, there is little empirical evidence regarding the income generating effect of Farmer Producer Organizations in India. Hence, a study entitled, “Performance Analysis of Farmers Producer Organizations of Uttarakhand” was carried out to document: (i) The genesis and organizational structure of FPOs, (ii) to study the performance of FPOs, (iii) to assess the socio-economic impact of FPOs on the members and (iv) to find out the constraints faced by FPOs. The study was carried out in Haridwar district of Garhwal region and Pithoragarh district of Kumaun region of Uttarakhand. All the eight FPOs in both the districts were studied. The respondents were selected by random sampling. Five office bearers, ten members and ten non members from each FPO were selected and the total sample size was 200. Descriptive research design was adopted for the study and data was collected by using pre tested interview schedule and past records of FPOs. It was found that four FPOs were in Grade A, which indicates that their performance was very good and they were ready for credit linkages. It was seen that one FPO was in Grade B, two were in Grade C and one was in Grade D. The study showed that majority of members and non members (56.25 %) were in the middle age and majority of the members and non members were men. It was found that majority of members (92.5%) and non members (85%) fall under above poverty line category. The study showed that about half of the members (51.2%) and maximum number of non members (43.75 %) belonged to the General Category and a large majority of members and non members were engaged in cultivation. It was also found that in case of members, maximum number were educated upto high school (42.5%) and in case of non members, maximum (40 %) were educated upto middle school. It was evident from data that 38.75 percent members and 45 percent of non members possessed 1-5 acres of land. It was also found that majority of the members (72.5%) were involved with one organization whereas 85 percent of the non members were not members of any social organization. It was also seen that majority of members and non members lived in pucca house and large majority of the members and non members had 1-2 draught animals. It was found that 53.75 percent members and 48.75 percent non members scored high on material possession and 66.25 percent of members had more than five members in the family, whereas 46.25 percent of the non members belonged to large families. During the study, it was found that 40 percent of members belonged to upper class and 45 percent of non members belonged to middle class. It was also found that there was significant difference in the socio economic status of members of FPO and non members. The results showed that, irrespective of the climatic conditions, crops grown, infrastructural conditions, etc; there was a significant difference in the socio economic status of members of FPOs and non members of hilly as well as plain areas. This showed that the FPOs have a direct and significant impact on the income and living conditions of the farmers. Majority of the members (78.75%) exhibited high level of social empowerment, followed by 21.25 percent with medium social empowerment. In case of non members, it was seen that the maximum of the non members (56.25%) were in the medium category of social empowerment. It was observed that there was significant difference in the social empowerment of members of FPO and non members. The results showed that social empowerment through the FPOs were seen in both the hilly as well as the plain areas. The farmer members were more socially empowered than the non members of the area. They were high on self confidence, more socially aware, had recognition in the area and availed more services and facilities than the non members. It was found that the major constraints faced by the officials were difficulty in getting and renewing the licenses, lack of sufficient number of vehicles for transportation of products to market, lack of price policy by the government, huge equity capital requirement for starting the FPO and lack of adequate working capital. Major constraints faced by members were inadequate transportation facilities, lack of proper infrastructure and processing facilities, low price for produce and distress sale, distant markets, shortage of working capital and lack of team spirit and coordination.
  • ThesisItemOpen Access
    Clinocopathological studies on nanosilver administered Wistar rats
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2021-12) Neeraj Kumar; Batra, Munish
    The present study investigated the pathological effects of nanosilver at NOAEL dose in Wistar rats for a period of 90 days. A total of 35 rats of 6 weeks age were divided randomly into group 1 (control group) and group II (treatment group). Group 1 rats were administered nanosilver orally at NOAEL dose (30 mg/kg). In group II rats, most of rats were dull and lethargic and there was decrease in feed intake. A significant time dependent decrease in body weight. Blood parameters shows decrease in packed cell volume (PCV), haemoglobin (Hb), and total erythrocyte count (TEC). Morphology of erythrocytes is normocytic hypochromic anaemia with increase central pallor. Erythrocytic indices shows significant changes including MCH and MCH values were significant increased while MCHC shows nonsignificant changes in treated rats as compared to control rats. Other blood parameters like total leucocyte count (TLC) and total thrombocyte count (TTC) shows significant increase in value in treated group as compared to control. In differential leucocyte count, lymphocyte count was significantly decreased but neutrophil showed significant increase in value while eosinophils, monocytes and basophils showed nonsignificant variation in both groups throughout experiment. Other blood parameters like absolute neutrophil count (ANC) showed significant increase in value while absolute lymphocyte (ALC) was significantly decrease in treated group as compared to control. In biochemical profile there was significant increase in serum aspartate aminotransaminase (AST), alanine aminotransaminase (ALT), and cholesterol while other parameters like total serum protein, serum gamma globulin, serum albumin, serum globulin, and serum glucose showed decrease in value in treated group as compared to control. Other biochemical parameters like serum calcium and serum phosphorus showed nonsignificant changes in both groups throughout experiment. Grossly group I rats shows non-significant changes while group II rats show red patches in liver, red discoloration in lungs and mild congestion in heart. Histopathologically, no significant lesions could be in any organ of group I rats while in group II rats revealed congestion, thrombus formation, and degeneration of hepatocytes in liver, thickening of interalvular wall, emphysema, congestion and increase number of mononuclear cells in lungs. kidneys revealed the congestion of glomerular capillaries, interstitial haemorrhage, and coagulative necrosis. Heart of group II rats revealed congestion, necrosis, and mild enema in cardiac muscle fibres. Other organs like , spleen, uterus and testis showed non-significant changes. Collectively, these observations indicated that nanosilver at NOAEL dose has toxic effects on heath including serum biochemicals and tissue changes. The observations regarding pathomorphological alterations indicates that liver, kidneys, and lungs, were the most affected organs in nanosilver toxicity. Mild effect on heart was also observed. Collectively our results suggest that nanosilver have toxic effects even at NOAEL dose on the repeated exposure. The use of silver nanoparticles should be used restricted or avoided.
  • ThesisItemOpen Access
    Clinical and radiographic study on surgical management of distal diaphyseal fracture in long bones by end threaded positive profile intramedullary pins and titanium elastic nails in dogs
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2021-08) Manish Kumar; Kandpal, Manjul
    The present study was conducted on 12 clinical cases of dogs of either sex with distal diaphyseal long bone fractures. The animals were randomly divided into two groups having equal numbers viz. Group A in which the fracture was stabilized with End threaded positive profile intramedullary pins and Group B by Titanium elastic nails. Diagnosis of the distal diaphyseal fracture done by preoperative clinical evaluation and radiography. Various parameters like clinic-physiological parameters like rectal temperature, heart rate, respiration rate, inflammation, pain score, lameness and weight bearing score, Haematobiochemical parameters like Hb, TEC, TLC,DLC & ALP were recorded in all the animals at various time interval. Clinico-physiological parameters did not show any significant difference between the both groups and were fluctuating within normal physiological range. Intra operative observation like duration of surgery, radiation exposure time, extent of manipulation and soft tissue damage, degree of technical difficulties, status of fracture reduction and fracture fixation was also recorded. The mean duration of surgery and intraoperative radiation exposure time significantly less in Group A compare to Group B. Lameness score was significantly less in group B compare to group A. The weight bearing score was significantly more in Group B as compare to Group A. Post-operative radiograph revealed intact implants with evidence of significant bridging callus and better radiographic healing grade in Group B as compare to Group A. Functional outcome score was significantly more in Group B as compare to Group A. The post-operative complication comprised of loosening and distal migration of pin in one case of Group A. The haematobiochemical parameters were within the physiological range and change in the mean values between the groups were statistically non-significant. For the physiological and anatomical repair of the fracture site, the titanium elastic nailing was found to be superior method than End threaded positive profile intramedullary pinning.Titanium elastic nailing resulted in early weight bearing and limb use along with better ability to withstand the stresses of weight bearing.
  • ThesisItemOpen Access
    Study on physico-morphology, biochemical constituents and effect of different levels of egg yolk on cryopreservation of Pantja buck semen
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2021-09) Rashmi; Gupta, H.P.
    Present study was conducted to assess the cryopreservation of extended Pantja buck semen in Tris-citric acid-fructose-egg yolk-glycerol (TCF-EY) extender containing 4 different levels (5, 10, 15 and 20%) of egg-yolk and to compare the neat, post diluted, post equilibrated and post-thawed quality of semen. Artificial Vagina (AV) method was used to collect semen from four reproductively mature, 2.5 to 4 years old, healthy breeding Pantja bucks. A total of thirty-two ejaculates were collected twice a week. After semen collection from all bucks, neat semen was examined for pH, volume, concentration, mass motility, live spermatozoa count, HOST, acrosome and abnormal spermatozoa percent. Thereafter semen samples were pooled and evaluated for some biochemical parameters viz; Malondialdehyde (MDA), Glutamic Oxaloacetic Transaminase (GOT), Glutamic Pyruvate Transaminase (GPT) and Glutathione Peroxidase (GSH-Px). Pooled semen sample was divided and diluted into four groups as D1[Neat semen + tris-fructosecitric acid-egg yolk (@5%)-glycerol], D2[Neat semen + tris-fructose-citric acid-egg yolk (@10%)-glycerol], D3[Neat semen + tris-fructose-citric acid-egg yolk (@15%)-glycerol] and D4[Neat semen + tris-fructose-citric acid-egg yolk (@20%)-glycerol]. Pooled semen samples were processed and evaluated without washing after dilution, post equilibration and after thawing for progressive motility, live and dead sperm percentage, sperm abnormalities, plasma membrane integrity (HOST) and acrosome integrity. Evaluation of MDA, GOT, GPT and GSH-Px was also done at post thawing stage. Biochemical parameters were evaluated in pooled semen before dilution and at post-thawing stage only. Dilutions were made according to the SOP and equilibrated at 5°C for 3 h and then the French mini (0.25 ml) straws were filled with semen and prefrozen in liquid nitrogen (LN2) vapours followed by stocking in LN2 container for seven days. After 7 days frozen semen straws were thawed in a water bath at 37˚C for 30 seconds and post thaw evaluation was done for above mention parameters. The mean value for neat seminal parameters of Pantja buck semen were recorded as volume 0.51±0.090 ml, pH 6.83±0.004, concentration 3.94±0.228, mass motility 4.49±0.078, live spermatozoa 84.75±1.75%, head abnormality (%) 2.13±0.057 %, mid piece abnormality 0.46±0.021 %, tail abnormality 4.85±0.29%, total abnormality (%) 7.48±0.34 %, plasma membrane integrity (%) 83.53±1.696 %, Intact acrosome (%) 82.63±1.760 %, MDA 2.22±0.16 (nmol/ml), GOT 127.36±0.556 Units/l, GPT 16.99±0.348 Units/l and GSH-Px 7.94±0.56 Units/ml. Significant difference (P< 0.05) was observed for all the parameters in different stages of freezing viz; post dilution, post equilibration and post thawing and in various diluters viz; D1, D2, D3 and D4. All seminal parameters except total abnormality showed higher value in D2 (10% EY) group at post dilution, post equilibration and post thawing stages compared to other groups. Likewise, significantly lower value for MDA, GOT, GPT and GSH-Px was observed in 10% egg yolk at post thawing stage. It was therefore concluded that the Pantja buck semen can be cryopreserved successfully at 10% egg yolk level (V/V) in Tris-citric acid-fructose-egg yolk -glycerol extender.
  • ThesisItemOpen Access
    Insilico characterisation and epitope profiling of differentially expressed plasma membrane proteins of ‘x’ and ‘y’ chromosome bearing bovine sperm
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2021-09) Sharma, Prachi; Sharma, Mridula
    The specific and differentially expressed plasma membrane proteins of bovine sex sorted semen were selected after reviewing numerous research publications on proteomic profile of sexed semen of bovines. The insilico characters such as physicochemical properties, primary, secondary, tertiary structures, membrane topology, sub-cellular localisation, domain analysis, protein interactions and epitope profiling, both linear and discontinuous peptides was done for all the proteins by using various bioinformatics tools and software. For X specific protein CLRN3, 6 and 4 and for Y chromosome specific protein, SCAMP1, 6 and 8 linear and discontinuous epitopes were predicted, respectively. The proteins were localized on Plasma membrane like SPACA1, Leucine-rich repeat and fibronectin type III domain containing 2, CLRN3, SCAMP1 and Uncharacterised Protein may act as antigenic protein to be used for further production of sex- specific antibodies and separation of X and Y sperm. The results of insilico characterisation and epitope prediction of various selected X and Y bovine sperm plasma membrane specific and differentially expressed proteins would be helpful in selection of some efficient antigenic proteins to be used for further X and Y sperm specific antibody production or synthesis of antibodies artificially.
  • ThesisItemOpen Access
    In silico studies on efficacy of various antibacterial drugs against bovine endometritis
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2021-09) Arya, Damini; Sharma, Mridula
    The present study was conducted with the objective to find effective drug against bacterial bovine endometritis using Molecular docking. The antibacterial property of selected plants (already studied in vivo and new) was studied its comparison with conventional antibiotics was done. The plants selected for study were Tulsi, Garlic, Aloe vera and Kalmegh, among them Tulsi and Garlic were previously studied in vivo and in vitro for treating bovine endometritis and Aloe vera and Kalmegh were selected on the basis of its potent antibacterial activity. The phytochemicals of Tulsi were thymol, eugenol, linalool, carvacrol, apigenin and caryophyllene. Phytochemicals of Garlic were allicin, ajoene, diallyldisulphide and diallyltrisulphide. Phytochemicals of Aloe vera were aloe-emodin, anthracene, campesterol, lupeol and sitosterol β. Phytochemicals of Kalmegh were andrographolide, 14-deoxyandrographolide, neoandrographolide and 3-O-β-D-Glucopyranosyl-14,19-Dideoxyandrographolide. Selection of bacteria (S. aureus and E. coli) for the present study was done on the basis of most predominantly found bacteria causing bovine endometritis as observed in previous studies. From S. aureus Gyrase B, DNA ligase, dehydrosqualene synthase and FtsA protein and from E. coli DNA gyrase, FtsZ and ZapD protein were selected. The selected bacterial proteins were docked against phytochemicals of plants. To conduct molecular docking the 3-D structure of receptors and ligands is needed. The structures of receptor or bacterial proteins were obtained from Protein Data Bank in PDB format. The 3-D structures of ligand were retrieved from PubChem and ChemSpider databases in SDF and Jsmol format respectively. Based on molecular docking it was concluded that, Aloe vera showed highest binding affinity followed by Kalmegh, Tulsi and Garlic i.e., -8.05, -7.55, -6.40 and -3.89 Kcal/mol respectively. When the antibacterial property of plants and antibiotics were compared it was concluded that the Aloe vera and Kalmegh showed higher binding affinity as compared to gentamicin (-6.95 Kcal/mol) and enrofloxacin (-6.97 Kcal/mol) against pathogenic proteins of Staphylococcus aureus and E. coli. On comparing binding energy obtained by each phytochemical of each plant it was observed that Apigenin of Tulsi, Ajoene of Garlic, Lupeol of Aloe vera and 3-O-β-D-Glucopyranosyl-14,19- Dideoxyandrographolide of Kalmegh showed the better efficacy as they had minimum binding energy against all / maximum bacterial proteins as compared to other phytochemicals of the respective plant. Thus, the combination therapy of Aloe vera, Tulsi and Kalmegh or the specific most efficient phytochemicals (Apigenin, Lupeol, sitosterol β, Anthracene, Neoandrographolide and 3-O-β-DGlucopyranosyl-14, 19-Dideoxyandrographolide) combination may be used for further in vivo or in vitro trials in a direction of effective drug discovery against bacterial bovine endometritis.
  • ThesisItemOpen Access
    To study the effect of feeding Moringa oleifera leaves on performance of Pantja goats
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2021-09) Taye, Thomas; Sanjay Kumar
    The present study was conducted on Pantja goats, which were reared at the Sheep and Goat Production Unit at the Department of Livestock Production Management, G.B.P.U.A.T, Pantnagar, Uttarakhand, during the month of March 2021 to May 2021, on two separate age groups, namely 3 months and 6 months to study the effect of feeding Moringa oleifera leaves on body weight, haematological parameters and faecal egg count of internal parasitic load on Pantja goats. A total of 24 animals were selected, 12 from 3months age group and 12 animals from the 6 months age group each containing 6 control and 6 treatment animals. The dietary supplement included were as follows- T0- Control (for 3months age group), T1 - Containing 80% concentrate + 20% dried moringa leaves (for 3months age group), T2- Control (for 6 months age group), T3- Containing 60 % concentrate + 40 % dried moringa leaves (for 6 months age group). Ad libitum grazing and water was allowed for all the experimental animals during the period of 60 days. Body weight was recorded fortnightly. Blood samples were collected at 0 and 60th day for estimation of different haematological parameters. Faecal samples were collected at 0, 3, 7, 14, 21, 28 and 60th day for faecal egg count. The salient findings observed from the current experiment was that there was a significant (P0.05) difference in neutrophil, monocytes, lymphocytes and ESR. Moreover, the feed cost has reduced to a minimum with the inclusion of dried moringa leaves in the diet of the Pantja goats in both the age group. From the current experimental study it can be concluded that replacing dried moringa leaves with the concentrate feed @ 20 and 40% in both the age group of 3 and 6 months respectively may be advised to improve the body weight, haematological parameters and faecal egg count in Pantja goats.
  • ThesisItemOpen Access
    Disposition kinetics and tissue residue study of enrofloxacin in broiler poultry
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2021-08) Swatilika; Ahmad, A.H.
    The present investigation was conducted to study the pharmacokinetics and tissue residue levels of enrofloxacin following administration of enrofloxacin by oral route in broilers as single dose (10 mg.kg-1 b.w.) and as multiple doses (10 mg.kg-1 b.w.) for five consecutive days and to calculate the correct dosage regimen to utilize optimal therapeutic limits in preventing and curing the disease. For the present study commercial enrofloxacin (10% w/v) preparation was administered at a dose rate of 10 mg.kg-1 b.w. orally as a single dose to nine birds and as a multiple dose to nine birds for five days. The plasma and tissue samples were collected at regular intervals and subjected to analysis by HPLC after appropriate processing. From the study it was revealed that the broiler birds showed the distribution half-life of 0.582±0.015h, a volume of distribution (Vp) of 8.53±0.194L.kg-1, an elimination half-life of 13.183±0.623h, a Cmax level of 1.535± 0.007 μg.mL-1 for enrofloxacin after single dose administration. Whereas, a Cmax level of 1.680± 0.010 μg.mL-1, distribution half-life of 0.717±0.102h, a volume of distribution (Vp) of 7.820±0.249L.kg-1, an elimination half-life of 11.888±1.021h was observed for enrofloxacin after first dose following multiple dose administration. A Cmax level of 1.704±0.002 μg.mL-1, distribution half-life of 0.717± 0.102h, a volume of distribution (Vp) of 9.029±0.006L.kg-1, an elimination half-life of 14.011±0.084h was observed for enrofloxacin after last dose following multiple dose administration. According to the results obtained in the pharmacokinetic study an individualized dosage regimen containing a priming dose of 22.262 mg.kg-1 b.w. and a maintenance dose of 16.660 b.w. mg.kg-1 over a period of 12 h was suggested for the enrofloxacin in broiler birds following single dose administration. Following single dose oral administration the highest residue concentration of enrofloxacin was observed in liver (0.427±0.055μg.g-1) followed by kidney (0.312±0.008 μg.g-1) at 24 hrs post administration of enrofloxacin. Minimum residue concentrations of enrofloxacin were detected in heart and lungs. In case of multiple dose oral administration the highest concentration of enrofloxacin was found in liver (0.579±0.071 μg.g-1) at 24 hrs which decreased to 0.078±0.0014 μg.g-1 at 72 hrs. After 72 hrs post administration, no residue of enrofloxacin could be detected in lungs following multiple dose oral administration. Ciprofloxacin could not be detected in the tissues following single dose study while ciprofloxacin residues were observed in tissues following multiple dose study.