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Govind Ballabh Pant University of Agriculture and Technology, Pantnagar

After independence, development of the rural sector was considered the primary concern of the Government of India. In 1949, with the appointment of the Radhakrishnan University Education Commission, imparting of agricultural education through the setting up of rural universities became the focal point. Later, in 1954 an Indo-American team led by Dr. K.R. Damle, the Vice-President of ICAR, was constituted that arrived at the idea of establishing a Rural University on the land-grant pattern of USA. As a consequence a contract between the Government of India, the Technical Cooperation Mission and some land-grant universities of USA, was signed to promote agricultural education in the country. The US universities included the universities of Tennessee, the Ohio State University, the Kansas State University, The University of Illinois, the Pennsylvania State University and the University of Missouri. The task of assisting Uttar Pradesh in establishing an agricultural university was assigned to the University of Illinois which signed a contract in 1959 to establish an agricultural University in the State. Dean, H.W. Hannah, of the University of Illinois prepared a blueprint for a Rural University to be set up at the Tarai State Farm in the district Nainital, UP. In the initial stage the University of Illinois also offered the services of its scientists and teachers. Thus, in 1960, the first agricultural university of India, UP Agricultural University, came into being by an Act of legislation, UP Act XI-V of 1958. The Act was later amended under UP Universities Re-enactment and Amendment Act 1972 and the University was rechristened as Govind Ballabh Pant University of Agriculture and Technology keeping in view the contributions of Pt. Govind Ballabh Pant, the then Chief Minister of UP. The University was dedicated to the Nation by the first Prime Minister of India Pt Jawaharlal Nehru on 17 November 1960. The G.B. Pant University is a symbol of successful partnership between India and the United States. The establishment of this university brought about a revolution in agricultural education, research and extension. It paved the way for setting up of 31 other agricultural universities in the country.

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  • ThesisItemOpen Access
    Pathogenesis of susceptible and resistant strains of Haemonchus contortus in sheep
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2007-01) Rajanish Kumar; Yadav, C.L.
    Haemonchus contortus is the most pathogenic gastrointestinal nematode of sheep and goats. The anthelmintics are the only practical method to reduce the menace of haemonchosis. The indiscriminate use of anthelmintics has led to emergence of resistant strain/subspecies of H. contortus throughout the world. There are numerous reports of benzimidazole resistance in H. contortus from the world as well as India. The present study was planned to investigate differences in biological behaviour and pathogenecity of susceptible and FBZ resistant strain of H. contortus. The prepatent period of 17-18 days was recorded for both the strains. The mean total worm recovery of susceptible (1777.5), resistant (2680) and naturally infected sheep (1565) were recorded. The sex ratio, body weight loss and establishment rates were also measured for the both strains. The faecal egg count for susceptible strain 1683±33.0, for resistant strain 3775±118.3 and 1850±99.9 for naturally infected sheep were observed. Significant difference were observed between susceptible and resistant strain for worm count, sex ratio and establishment rates. The degree of reduction in PCV and Hb% indicated pathogenecity of worms but no significant differences were observed between susceptible and resistant strains. Peripheral eosinophil count and total erythrocytic count indicated significant difference between the two strains. The erythrocytic indices indicated normocytic, normochromic anaemia. The total iron levels of resistant strain infected group decreased significantly as compared to susceptible strain infected group. The total serum protein reduced from 6.95 ± 0.37 to 582 g/dl, 6.79 ± 0.20 to 3.53 ±0.28 and 6.88 ± 0.19 to 4.92 ± 0.47 from the day of infection to 49 DPI in susceptible, resistant strain infected sheep and sheep infected by grazing, respectively. The serum albumin levels also mirrored similar trends to total serum protein. Thus, hypoproteinaemia and hypoalbuminaemia was significantly more in resistant infected sheep. The serum pepsinogen level increased from 1.10 ± 0.23 to 2.67 ± 0.12 IU tyrosine in susceptible strain, 1.19 ± 0.27 to 3.23 ± 0.31 in resistant strain infected sheep and 1.37 ± 0.25 to 3.22 ± 0.03 IU tyrosine in sheep infected by grazing. Significant differences were recorded with regards to serum pepsinogen level between the two strains indicating higher abomasal damage in resistant strain infected sheep. Thus, it can be inferred that both the strains are different in their biological behaviour and the pathogenecity, with resistant strain being more pathogenic than susceptible strain.
  • ThesisItemOpen Access
    Immunological and molecular characterization of Marek’s disease virus isolated from field outbreaks
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2007-07) Chamling, Prerna; Umapathi, V.
  • ThesisItemOpen Access
    Clinicopathological studies on effects of brass industry effluent in animals
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2007-01) Manjunatha, K.N.; Thathoo, A.K.
    The present experiment was conducted to study the clinicopathological effects of Brass industry effluent in animals. For this, various samples (effluent, soil and fodder) were collected around the brass industries located in Moradabad and subjected to physico-chemical analysis. Toxicity of the effluent was studied by using mice as an experimental animal model. The physico-chemical analysis of effluent samples showed significantly (P<0.05) increased levels of COD, BOD, TS, TDS, TSS, and heavy metals as compared to control. There was significantly higher levels of metals in soil (Zn, Cu, Cd and Pb) and in fodder (Zn, Cu, Mn, Fe, Cd, and Pb) samples that were collected from and around the industries compared to the control site. Animal experimentation revealed significant effects of the effluent on body and organ weights, hematological and biochemical parameters. There was significant decrease in body and liver weight as compared to control. Total erythrocyte count was increased significantly, whereas total leukocyte count decreased significantly as compare to the control. There was decrease in percent monocyte (%) and percent increase in the neutrophils as compared to the control. On serum biochemical examination it was evident that there was significantly (P<0.05) higher levels of BUN, Creatinine, AST and ALT, where as significant decrease in total protein and albumin levels as compared to the control. The levels of zinc, cadmium and lead in serum and liver samples of mice exposed to effluent were significantly (P<0.05) increased as compared to the control. The histopathological examination of various organs revealed, congestion, edema, adhesion of glomerulus to the Bowman‟s capsule, interstitial nephritis, extensive degeneration of tubular epithelium, proteinacious casts, atrophy of glomerulus, glomerular hemorrhages and necrosis in kidneys. Liver showed congestion, edema, vacuolar degeneration and dilatation of sinusoids. Congestion hemorrhages and focal areas of necrosis were seen in spleen. Lungs showed congestion, edema, emphysema, interstitial pneumonia, bronchopneumonia and focal areas of accumulation of mononuclear cells around bronchus. Testes showed mild congestion, edema, degeneration, detachment of cells from basement membrane, impaired spermatogenesis and atrophy of seminiferous tubules. In the brain, congestion, edema, perivascular cuffing, chromatolysis, neuronophagia and extensive vacuolations were observed. Based on above findings, it may be concluded that the effluent from brass industries have higher levels of COD, BOD, TS, TDS, TSS and heavy metals. Effluent discharged from these industries has increased the heavy metal concentration in and around the areas of these industries (soil and fodder). From the animal experiment, it is suggested that the brass industry effluent causes significant chronic health effects on animals.
  • ThesisItemOpen Access
    Isolation and characterization of fowlpox virus of poultry
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2007-07) Madanpal; Rao, V.D.P.
    Fowl pox is a contagious and slow spreading viral disease. It affects the birds of all age, sex and breeds. The disease is manifested in three different clinical forms–cutaneous, diphtheritic and oculo nasal forms. The mucosal lesions involving the mouth, esophagus and trachea can be confused with other respiratory diseases like infectious laryngotrachaetis, coryza etc. Keeping in view of the impact of disease on economics of poultry industry, the present study was undertaken to isolate the virus from scab lesions of birds of a poultry farm near Barielly following isolation of virus on chorioallantoic membrane (CAM), of developing chicken embryos, further characterization was carried by studying cytopathogenicity in cell culture and sero diagnostic tests. It was observed that virus was successfully adapted to CAM, CEF cells as well as in BHK 21 cell line. Characteristic pock lesion in CAM and CPE in unstained and stained preparations confirmed the presence of virus. In MGG staining, the cytopathic changes characterized by rounding of cells 24 hrs PI and the cytoplasmic vacuolation and syncytia formation by 48 hrs PI. In few cells, the nucleus occupied eccentric position and degenerative changes in the nucleus characterized by fragmentation of nuclear membrane in the infected CEF cells while the cytopathic changes in infected BHK 21cells were characterized by rounding of cells 36 hrs PI and the cytoplasmic vacuolation and syncytia formation by 48 hrs PI. The infectivity titre was calculated to be log104.25/ml (EID50/ml) on CAM and log10 9.79/ml (TCID50/ml) in CEF cell culture. The agar gel precipitation test (AGPT) revealed the precipitation band, which confirms the presence of antigen and antibody. Counter immunoelectrophorasis (CIE) showed a precipitation line within one hr of electrophoretic run. The indirect fluorescent antibody technique (IFAT) was used to demonstrate the virus in infected cell culture and cell line. The infected chicken embryo fibroblast cells revealed small particulate fluorescence in the cytoplasm of the cells. These tests confirmed that the virus isolate as fowlpoxvirus SDS-PAGE analysis of cell culture supernatant infected with FPV isolate revealed 11 polypeptides with molecular weights ranging from 120k Da to 15 kDa.
  • ThesisItemOpen Access
    Studies on tubal patency in cattle and buffaloes
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2007-07) Ansari, Gulam Muhammad; Gupta, H.P.
    Fallopian tube, an important and vital link between the ovary and uterus is concerned for the both transport of gametes and fertilization. So, for these processes, tubal patency must be maintained. Thus, an investigation was conducted to study tubal patency in buffaloes and cows. There were two parts in this study, in vitro and in vivo. In vitro study was conducted on morbid buffalo genitalia. After recording gross abnormalities of the genitalia, utero-tubal insufllation was conducted to study tubal patency. Out of 309 fresh morbid buffalo genitalia examined, 7.76% bursal adhesion, 5.82% follicular cyst, 5.17% luteal cyst, 15.53% ovarian hypoplasia, 1.96% paraovarian cyst, 3.88% hydrosalpinx and 23.94% kinked cervix were observed. Out of 309 fresh morbid buffalo genitalia studied, 39.15% unilateral patency, 49.51% bilateral patency and 11.32 % bilateral blockade were noted. These genitalia were also subjected to salpingography by injecting lead oxide-soap-water suspension in both the salpinx and the course of the tube was delineated by this method. Tubal histopathology was also accomplished. The salient pathological lesion causing blockades were pyosanlpingitis and hydrosalpingitis. In vivo study was conducted on 21 repeat breeding cows for assessment of gross abnormalities and tubal patency by phenolsulphonthalein dye test and uterotubal insufflation test. Out of 21 repeat breeding cows, PSP dye test gave result of 28.57% unilateral patency, 61.90% bilateral patency and 9.52% bilateral blockade. However, 23.80% unilateral patency, 71.42% bilateral patency and 4.76% bilateral blockade was found in insufflation test. From this study it is concluded that: Pathological lesions, like bursal adhesion, hydrosalpinx, suppurative and non-suppurative salpingitis has direct influence over the tubal patency, but other affections like ovarian hypoplasia and para-ovarian cyst failed to cast such influence over tubal patency of morbid genitalia. About 50% morbid genitalia had tubal blockade, either unilateral or bilateral. Salphigography remains at its infancy for the detection of tubal patency in buffalo. Pyosalpingitis and hydrosalpingitis were salient pathological lesions associated with ovoductal blockadge. Phenolsulphonphthalein (PSP) dye test and air-insufflation test are the two non-invasive and sensitive methods for the assessment of tubal patency in repeat breeding cows.
  • ThesisItemOpen Access
    Detection and quantification of chlorpyrifos and endosulphan residues in dead animal’s using high performance liquid chromatography
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2007-05) Tyagi, Amit; Dixit, V.P.
    In the present study, about 168 tissue samples were analyzed for the presence of endosulfan and chlorpyrifos residues and 74 for presence of monocrotophos using the standardized method. The residues were extracted by treating with acetonitirile followed by liquid-liquid partition with sodium sulfate solution (2.5%): dichloromethane. The extracts obtained after dehydration on sodium sulfate column were cleaned up by performing adsorption chromatography on alumina column. The detection and quantification of these residues was carried out with the help of High Performance Liquid Chromatography using Diode Array Detector. Five (7.14 %) out of 70 muscle, 2 (2.85 %) of 70 liver and none of 70 kidney tissues were detected positive for endosulfan α residues. Out of these none of the samples violated the prescribed limits given by CODEX. Only one (1.42 %) of muscle tissue sample out of 70 was detected positive for endosulfan β residues. Out of these also none of the samples violated the prescribed limits given by CODEX. Seven (10.00 %) of muscle tissue, 6 (8.57 %) of kidney and 2 (2.85 %) of liver were detected positive for endosulfan sulfate residues. Out of these total samples none of the samples violated the prescribed limits given by CODEX. Chlorpyrifos residues were detected in 5 (7.14 %) of 70 muscle samples, 4 (5.71 %) of 70 liver and none of kidney tissue was detected positive. None of these samples also violated the prescribed limits given by CODEX. All of the 121 samples analyzed for presence of monocrotophos were found negative.
  • ThesisItemOpen Access
    Studies on mineral profile in feeds, soil and animals in Haridwar district of Uttaranchal
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2006-01) Tiwary, Manoj Kumar; Tiwari, D.P.
    A survey was conducted in four villages, two each from Roorkee and Laksar Tehsils, of Haridwar district of Uttaranchal in order to find out the existing livestock practices, macro and micro nutrients profile in soil, feeds and fodder and animals and also to establish the correlations among soil, plant and animals. Fifteen farmers under three major categories on land holding basis i.e. landless (0 acre), small (2-5 acres) and large (>5 acres) from each village were selected, from where the above informations and samples of soil, feeds, fodder and blood of animals of different physiological status were collected. The collected feed, soil, water and blood samples were subjected for macro and micro nutrient analysis. The CP (23.41 %) and total ash (16.91 %) content were highest in cowpea green whereas, lowest in wheat straw (3.33 % and 7.70 %), in Roorkee Tehsil. While in concentrate, CP content was highest in soybean cake. Animals of the target area showed negative balances in DM, CP and ME intakes. Aneostrus problems were higher in the villages of Roorkee Tehsil as compared to Laksar Tehsil whereas, incidence of repeat breeding in cattle and buffaloes were higher in villages of Laksar Tehsil than Roorkee Tehsil. Only soil of Roorkee Tehsil was found deficient in calcium while rest minerals were found above their respective critical levels. In concentrate ingredients, all minerals except copper such as calcium, phosphorus, magnesium, zinc, iron, cobalt, manganese and selenium were above their respective critical levels with corresponding values as 0.38 ± 0.01, 0.36 ± 0.02, 0.11 ± 0.01 per cent, 53.77 ± 1.78, 279.95 ± 6.96, 0.31 ± 0.04, 65.93 ± 4.34 and 0.15 ± 0.02 ppm, respectively, whereas, copper was found 7.74 ± 0.51 ppm, below its critical level. Likewise, in roughage, minerals such as calcium, magnesium, zinc, iron, cobalt, manganese and selenium were above the critical levels with corresponding values as 0.36 ± 0.03, 0.10 ± 0.01 per cent, 53.50 ± 6.47, 297.78 ± 21.73, 0.49 ± 0.12, 47.01 ± 8.01 and 0.18 ± 0.02 ppm, respectively, whereas, phosphorus and copper were 0.20 ± 0.03 per cent and 5.06 ± 0.75 ppm, respectively, below their respective critical level. The blood serum mineral concentration except for copper and phosphorus i.e. calcium, magnesium, zinc, iron and manganese (10.43 ± 0.22 mg/dl, 1.65 ± 0.14 mg/dl, 1.54 ± 0.11 ppm, 1.97 ± 0.19 ppm and 0.54 ± 0.02 ppm) were found to be above their respective critical levels and were not deficient. The serum phosphorus (3.79 ± 0.21 mg/dl) and copper (0.46 ± 0.07 ppm) were deficient with their values falling below their respective critical levels. Soil-plant-animal correlations for different minerals did not show clear cut picture regarding their accumulation in plants and utilization by the animals. Research findings revealed for the supplementation of phosphorus, copper as well as magnesium in the selected areas of Haridwar.
  • ThesisItemOpen Access
    Comparative study of different marination treatments and methods of cooking in the preparation of hurdled processed chevon curry
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2006-10) Sisira Piyasiri, H.H.A.; Tanwar, V.K.
    Preparation of chevon curry is not an uncommon practice in normal domestic house holds and restaurants in the Asian countries including India. However main problems encountered in chevon curry preparation are toughness of meat chunks and shorter shelf life of the product. There for attempts have been made to standardize the technology for production of organoleptically acceptable chevon curry with longer storage life by applying various hurdles such as temperature, pH and preservatives. The meat chunks were first marinated with different marinade ingredients for 18 hours at 4±1oC.The different marinaded meat samples were cooked with two cooking methods viz microwave cooking and conventional cooking. In the first part of the study best marinade treatment was selected from each cooking method on the basis of sensory scores. The marinade treatment 1 from the conventional cooking and marinade treatment 3 from the microwave cooking method were rated highest and these two groups with the two controls were subjected to further studies. The physico chemical, microbiological and sensory characteristics of chevon curries treated with different marinades and cooked with two cooking methods were studied at different storage time intervals under refrigeration temperature. The chevon curry marinated with preservatives was rated significantly higher in all sensory attributes and shelf life compared to chevon curry marinated with out preservatives. Chevon curry prepared with out preservatives in marinades spoiled after 10 days at refrigeration storage. However chevon curries marinated with preservatives and combined different hurdles had the shelf life beyond the 15th day of similar storage. The chevon curries marinated without preservatives were high in microbiological load, low in sensory scores and high in TBA values compared to chevon curries marinated with preservatives during storage. In comparative study of chevon curry cooked by microwave and conventional methods, the cooking yield was significantly higher in microwave cooked products. In sensory quality aspects conventional cooked chevon curry was rated higher than the microwave cooked product in all the sensory attributes excluding the juiciness and colour scores. In microbiological aspects chevon curry prepared by conventional cooking method, was better than microwave cooking. It was observed that yeast and mould counts of conventional cooked chevon curry were significantly lower than microwave cooked chevon curry. From the results it can be concluded that highly palatable and stable chevon curry can be prepared by applying different hurdles such as preservatives and adjusted pH. Conventional cooked chevon curry was better in overall acceptability and shelf life compared to microwave cooked chevon curry.
  • ThesisItemOpen Access
    Studies on vaccine strain of Fowlpoxvirus with special reference to its adaptation in primary cell cultures and its characterization
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2006-08) Tripathi, Ashok Kumar; Rajesh Chandra
    Fowlpox is an economically important and widespread disease of poultry that causes heavy morbidity and mortality in the affected birds resulting in heavy losses in the form of low egg and meat production. It affects the birds of all age, sex and breeds. The disease is caused by Avipoxvirus of the family Poxviridae. The disease is manifested in three different clinical forms–cutaneous, diphtheritic and oculo nasal forms. The mucosal lesions involving the mouth, esophagus and trachea can be confused with other respiratory diseases like infectious laryngotrachaetis, coryza etc. Keeping in view the economic impact of disease on poultry industry, the present study was undertaken to adapt the vaccine strain of FPV in chorioallantoic membrane, chicken embryo fibroblast cell culture as well as in chicken kidney cell culture and evaluate the various serological tests for rapid diagnosis of FPV infection. Fowlpoxvirus was successfully adapted to CAM, CEF and CK cells. Characteristic pock lesions on CAM and CPE in unstained and stained preparations confirmed the presence of virus. In MGG staining, the cytopathic changes were characterized by rounding of cells 12 hrs PI and the cytoplasmic vacuolation and syncytia formation by 18 hrs PI. In few cells, the nucleus occupied eccentric position and degenerative changes in the nucleus were characterized by fragmentation of nuclear membrane in the infected CEF cells, while the cytopathic changes in infected CK cells were characterized by rounding of cells 36 hrs PI and the cytoplasmic vacuolation and syncytia formation by 48 hrs PI. The infectivity titre was calculated to be log107.14 EID50/ml on CAM and log10 9.25TCID50/ml in CEF cell culture. The agar gel immunodiffusion test revealed the precipitation band, which confirms the presence of antigen and antibody. The serum neutralization test using the beta (constant virus serum dilution) procedure, revealed an antibody titre of 1:160. CIE showed a precipitation line within one hr of electrophoretic run. The indirect fluorescent antibody technique (IFAT) demonstrated the presence of virus in infected cell culture. The infected embryonic chicken kidney cells as well as the infected chicken embryo fibroblast cells revealed small particulate fluorescence in the cytoplasm of the infected cells.