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Govind Ballabh Pant University of Agriculture and Technology, Pantnagar

After independence, development of the rural sector was considered the primary concern of the Government of India. In 1949, with the appointment of the Radhakrishnan University Education Commission, imparting of agricultural education through the setting up of rural universities became the focal point. Later, in 1954 an Indo-American team led by Dr. K.R. Damle, the Vice-President of ICAR, was constituted that arrived at the idea of establishing a Rural University on the land-grant pattern of USA. As a consequence a contract between the Government of India, the Technical Cooperation Mission and some land-grant universities of USA, was signed to promote agricultural education in the country. The US universities included the universities of Tennessee, the Ohio State University, the Kansas State University, The University of Illinois, the Pennsylvania State University and the University of Missouri. The task of assisting Uttar Pradesh in establishing an agricultural university was assigned to the University of Illinois which signed a contract in 1959 to establish an agricultural University in the State. Dean, H.W. Hannah, of the University of Illinois prepared a blueprint for a Rural University to be set up at the Tarai State Farm in the district Nainital, UP. In the initial stage the University of Illinois also offered the services of its scientists and teachers. Thus, in 1960, the first agricultural university of India, UP Agricultural University, came into being by an Act of legislation, UP Act XI-V of 1958. The Act was later amended under UP Universities Re-enactment and Amendment Act 1972 and the University was rechristened as Govind Ballabh Pant University of Agriculture and Technology keeping in view the contributions of Pt. Govind Ballabh Pant, the then Chief Minister of UP. The University was dedicated to the Nation by the first Prime Minister of India Pt Jawaharlal Nehru on 17 November 1960. The G.B. Pant University is a symbol of successful partnership between India and the United States. The establishment of this university brought about a revolution in agricultural education, research and extension. It paved the way for setting up of 31 other agricultural universities in the country.

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  • ThesisItemOpen Access
    Studies on genetic characterization of genus Theileria in large ruminants of Uttarakhand state
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2021-09) Sharma, Deepesh; Vatsya, Stuti
    Bovine theileriosis (BT) caused by Theileriaannulata/Theileriaorientaliscauses significant economic losses to the livestock industry in India. The present study was thus planned with the objective of standardizing molecular detection of theileriosis in bovines of Uttarakhand and understanding the genetic variability of genus Theileria based on 18SrRNA, Tams 1 and MPSP gene sequence analysis. Prevalence of BT was studied in large ruminants of different zones of Uttarakhand state from May, 2019 to June, 2021. Out of a total of 1006 blood samples (754 from cattle and 252 from buffaloes) examined, 283 (28.13%) blood samples were found positive (cattle-32.23%, buffaloes- 15.87%) for BT. Agro-climatic area-wise,%prevalence of BT was found to be higher in tarai area {30.93 (cattle-35.96; buffaloes-16.58)} as compared to hilly area {23.56 (cattle-26.05; buffaloes-14.23%)} with the highest being in district Haridwar (cattle-39.23; buffaloes-20.00) of tarai and Nainital (cattle-29.65; buffaloes-22.00) of hill area. Seasonwise% prevalence in large ruminants was found maximum in summer (34.64) followed by rainy (26.63) and then winter (9.42). Age-wise, overall% prevalence of BT was found more in animals > 3 years followed by 1-3 years and least in animals <1 year of age (cattle-40.50, 32.69, 9.93; buffaloes-20.97, 16.67, 3.57, respectively). Overall sexwise% prevalence of BT was found more in female (35.57 and 17.21) than male (16.15 and 8.11) cattle and buffaloes, respectively. During the present study, no Hyalomma tick could be recovered from animals. Rhipicephalusmicroplus was the main tick infesting on the body of animals. Thin blood smear examination (TBE) revealed overall% prevalence of BT as 24.13 and 10.71 in cattle and buffaloes, respectively. AS-PCR assay of partial 18S rRNA gene yielded an amplicon of 1098 bp. AS-PCR assay detected an overall% prevalence of 28.13 of BT. (cattle-32.23; buffaloes-15.87). PCR method was found more sensitive than TBE. The overall% prevalence of BT in cattle was 24.13 and 32.23 and in buffaloes 10.71 and 15.87 using TBE and PCR assay, respectively. Amplification of partial Tams 1 gene for detection of T. annulatayielded amplicon of 785 bp. Overall% prevalence of Bovine Tropical Theileriosis (BTT) infection in bovines of Uttarakhand was 22.96 {cattle-26.12 (Haridwar-30.00; Udham Singh Nagar-28.88; Dehradun-26.80; Pauri-16.07); buffaloes-13.49 (Nainital-20.00; UdhamSingh Nagar-14.54; Pauri-8.69)}. AS-PCR assay to amplify partial MPSP gene to detect T. orientalis yielded amplicon of 776 bp. The overall% prevalence of Bovine Benign Theileriosis (BBT) infection in bovines of Uttarakhand was 5.17 {cattle- 6.10(Haridwar-9.23, UdhamSingh Nagar-6.95, Dehradun-6.54, Champawat and Pauri-1.79); buffaloes-2.38 (UdhamSingh Nagar-5.45, Haridwar-3.77, Nainital-2.00, Dehradun, Champawat and Pauri-0.00)}. The overall% prevalence of T. orientalis was recorded to be 18.40 (cattle-18.93; buffaloes -15) and that of T. annulata as 81.60 (cattle- 81.07; buffaloes-85). The% sensitivity and specificity of TBE and PCR assay was 73.85,100 and 100, respectively. The partial 18S rRNA gene sequence of T. orientalis obtained from Dehradun_S1 isolate from cattle showed% identity in the range of 95.93-99.38, Haridwar_S2 isolate 97.09-99.38, UdhamSingh Nagar_S3 isolate 99.85-100 and Nainital_S4 isolate 98.10-98.20 with different cattle T. orientalis isolates. The sequence analyses of the partial 18S rRNA gene revealed high level of polymorphism between regions. The sequence analysis of the partial Tams 1 gene revealed that UdhamSingh Nagar_T2 and Haridwar _T3 isolates Theileria showed common nucleotide variations at 27 sites (other variations at 48 sites in UdhamSingh Nagar_T2 isolate and at 61 sites in Haridwar_T3 isolate). Phylograms of the partial Tams 1 gene sequences revealed that Udham Singh Nagar_T2 isolate from buffalo of Kumaun region and Haridwar_T3 isolated from cattle of Garhwal region shared common ancestors with T. annulata isolates of cattle from Uttarakhand state. The nucleotide sequence analysis of the partial MPSP gene revealed high level of polymorphism between regions (nucleotide variations at 14 sites in Haridwar M4 isolate and in UdhamSingh Nagar isolate at 61 sites). The phylogenetic analysis of partial MPSP gene sequences of T. orientalis revealed that UdhamSingh Nagar_M2 isolate from buffalo in our study belonged to T. orientalis of N1 type and Haridwar_M4 isolate to T. orientalis of Type 5. Phylogram analysis also revealed that UdhamSingh Nagar_M2 and Ikeda strain of Japan have common ancestor which is a highly pathogenic strain of T. orientalis. This is the first report describing the circulation of T. orientalis in large ruminants (6.10% cattle and 2.38% buffaloes) of Uttarakhand state. Both T. annulata and T. orientalis are circulating in Uttarakhand including two subtypes of T. orientalis(Type 5 and N1Type). It is suggested that the findings of the present study can be useful in devising managemental and control strategies against bovine theileriosis in Uttarakhand state.