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Govind Ballabh Pant University of Agriculture and Technology, Pantnagar

After independence, development of the rural sector was considered the primary concern of the Government of India. In 1949, with the appointment of the Radhakrishnan University Education Commission, imparting of agricultural education through the setting up of rural universities became the focal point. Later, in 1954 an Indo-American team led by Dr. K.R. Damle, the Vice-President of ICAR, was constituted that arrived at the idea of establishing a Rural University on the land-grant pattern of USA. As a consequence a contract between the Government of India, the Technical Cooperation Mission and some land-grant universities of USA, was signed to promote agricultural education in the country. The US universities included the universities of Tennessee, the Ohio State University, the Kansas State University, The University of Illinois, the Pennsylvania State University and the University of Missouri. The task of assisting Uttar Pradesh in establishing an agricultural university was assigned to the University of Illinois which signed a contract in 1959 to establish an agricultural University in the State. Dean, H.W. Hannah, of the University of Illinois prepared a blueprint for a Rural University to be set up at the Tarai State Farm in the district Nainital, UP. In the initial stage the University of Illinois also offered the services of its scientists and teachers. Thus, in 1960, the first agricultural university of India, UP Agricultural University, came into being by an Act of legislation, UP Act XI-V of 1958. The Act was later amended under UP Universities Re-enactment and Amendment Act 1972 and the University was rechristened as Govind Ballabh Pant University of Agriculture and Technology keeping in view the contributions of Pt. Govind Ballabh Pant, the then Chief Minister of UP. The University was dedicated to the Nation by the first Prime Minister of India Pt Jawaharlal Nehru on 17 November 1960. The G.B. Pant University is a symbol of successful partnership between India and the United States. The establishment of this university brought about a revolution in agricultural education, research and extension. It paved the way for setting up of 31 other agricultural universities in the country.

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Subject: Agricultural Biotechnology × Author: Dawane, Akashata P. × End date: 2022 × Type: Thesis × Thesis Type: M.Sc ×
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    ThesisItemOpen Access
    Expression analysis of different genes of MAPK cascade and terpene synthase 21 gene against aphid attack on Brassica juncea (var.) Varuna and in-silico characterisation of terpene synthase 21 gene
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2018-06) Dawane, Akashata P.; Gohar Taj
    Brassica juncea is a major oilseed crop of India and produced on a large scale over the globe. It is a major source of vegetable oil and protein meal. It is mostly affected by a variety of abiotic and biotic stress that affects its potential yield and cause a huge loss of crop productivity annually. One such biotic stress is attack of mustard aphid (L. erysimi) a polyphagous destructive pest, which cause 9-96 % of yield loss in mustard annually. It causes loss in terms of both productivity and oil content. MAPK is a major defense related signalling pathway in eukaryotes which has been documented for its role in providing defense against variety of biotic and abiotic stresses in plants. MAPK signalling pathway is reported to be activated as first line of defense and play an active role during attack of insect herbivore. This pathway further leads to the activation of various defense related genes which are regulated by different transcription factors. Among these transcription factors WRKY also plays a major role in warding off insect herbivore by increasing the synthesis of various secondary metabolites. WRKY are reported to activate or supress the synthesis of these secondary metabolites. One such class of secondary metabolites is Terpene synthase. Terpene synthase release a volatile compound called as (E, E) -farnesene that attracts the natural enemy of insect pest and thus provide defense against herbivore attack. In present study, Brassica juncea cv varuna plants were evaluated against aphid (L. erysimi) infestation to study the defense pathway activated after the attack. The effect of aphids on growth parameters of plants was evaluated with expression level of MAPK cascade, WRKY transcription factors and Terpene synthase 21. The interaction of these genes were studied to derive the possible pathway activated during the aphid attack. During the present course of study, Terpene synthase 21 gene sequence of Brassica rapa was retrieved from NCBI and In silico study was performed to check its phosphorylation site, secondary structures and conserved or functional domain. Terpene synthase 21 was reported to be present in Brassica juncea by quantitative PCR analysis.