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Tamil Nadu Veterinary and Animal Sciences University, Chennai
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ThesisItem Restricted HEMATOPOIETIC PROGENITOR CELL CHARACTERIZATION IN CANINES(2018) Nandhini, S; Mangala Gowri, A; Meenambigai, TV; Baranidharan, GR; TANUVASBlood cells are responsible for constant maintenance and immune protection of every cell type of the body and this relentless and brutal work requires cells that have the greatest powers of self-renewal and are designated as Hematopoietic progenitor cells (HPCs). Peripheral blood stem cells in circulation have become the most common source of hematopoietic stem cells intended for transplantation after minimal manipulation. In vitro and in vivo animal studies have shown that the enriched CD34+marrow cells of different species can give rise to the multiple blood cell lineages and may provide long-term hematopoiesis, suggesting that CD34 could be regarded as a cell surface marker of primitive hematopoietic stem cells. This has made it possible to enrich HSCs from mice and human beings for molecular characterization and transplantation purposes. Homeobox (Hox), sonic hedgehog (SHH), and Wingless-type MMTV integration site family (Wnt) are known to modulate the self-renewal and expansion of hematopoietic progenitor/stem cells in humans and mice. Unlike cytokines, Hox, SHH, and Wnt are highly conserved among species from flies to humans but studies regarding the self-renewal and expansion of the HSC are extremely limited in dogs. Hence in the present research, a rapid CD34+population enrichment and immuno separation protocol has been standardized in canine blood. The expression of signaling molecules for self renewal and colony forming potential of the isolated CD34+ population of cells from Canine blood showed that they were cells with proliferation and self renewal potential.ThesisItem Restricted PROPIDIUM MONOAZIDE (PMA) QUANTITATIVE POLYMERASE CHAIN REACTION (q PCR) FOR DETECTION OF LIVE BACTERIA IN FOOD SAMPLES(2018) Shathish Sharma, BJ; Rathnapraba, S; Meignanalakshmi, S; TANUVASThe present study was aimed to investigate the presence of foodborne bacterial pathogens in meat products. A total of 100 meat samples (including raw chicken, chicken nuggets, sausage, Kheema, cutlet, meat balls, pakoda, raw mutton, mutton balls, pork sausage, kheema, khabab, ham bacon, beef khabab, cubes, kheema and sausage) have been collected from different retail outlets in Chennai. The overall occurrence of B. cereus, S. aureus and E. coli in different meat samples were found to be 15%, 20% and 14%respectively. All the presumptive colonies were subjected to molecular screening by targeting the Hemolysin gene, fem A gene and mal B gene in B. cereus, S. aureus and E. coli respectively. Based on the molecular characterization, about 11, 5 and 5 isolates of B. cereus, S. aureus and E. coli were identical in screened meat samples. The isolates from the meat and meat products were subjected to antibiotic susceptibility test and the results revealed that all the isolates showed 100% resistance to Vancomycin and Penicillin. Staphylococcus aureus shows 100% resistance to Methicillin and 68.8% sensitivity to ciprofloxacin. In B. cereus they showed 100% sensitivity to methicillin whereas E. coli showed 100% resistance to ampicillin and 100% sensitivity to ciprofloxacin. PMA-qPCR was developed to quantify the viable foodborne bacterial pathogen in meat products. In the present study 2.5 mM concentration of PMA dye was used, which will help to prevent the amplification of DNA from the dead cells. The developed PMA-qPCR had the detection limit of 1X101.25 CFU/g, 1X100.80CFU/g and 1X 100.4 CFU/g forB. cereus, S.aureus and E. coli respectively. These PMA qPCR results were compared with conventional qPCR. These results indicated that the developed PMA-qPCR was more sensitive than the conventional culture method. The sensitivity, specificity and accuracy of developed PMA-qPCR were calculated using Kappa statistics method. The sensitivity of PMA-qPCR for Staphylococcus aureus, Bacillus cereus and Escherichia coli were found to be 60%, 54.4% and 60% respectively. Specificity of the PMA qPCR was found to be 66.7%, 66.7% and 64.4% for Bacillus cereus Staphylococcus aureus and Escherichia coli respectively. The PMA q-PCR could discriminate among viable and dead bacteria it detect only live bacteria from the food samples.ThesisItem Open Access IN SILICO APPROACHES TO STUDY THE EFFECT OF A1 AND A2 MILK VARIANTS ON TYPE 1 DIABETES MELLITUS INCIDENCE(TANUVAS, 2016) Ophelia, E. Felix Anto; Sujatha, PL; Kumarasamy, P; Cauveri, D; TANUVASCow’s milk [3-casein is a protein product of CSN2 gene, containing 209 amino acids. There arc many genetic variants of β-casein among which Al and A2 are recurrent which differs only by the presence of histidine in Al and proline in A2 variant at position 67. Due to the presence of histidine, the Al variant is more susceptible to the enzymatic digestion. As a consequence of this enzymatic digestion in the gastrointestinal tract, a bioactive peptide of 7 amino acids known as β-casomorphin 7 (BCM7), is released and absorbed in infants, as their gastrointestinal tract is more permeable As the name indicates, BCM7 elicit an opiate-like activity.ThesisItem Open Access IN SILICO ANALYSIS OF ACTIVE PRINCIPLES PRESENT IN GYMNEMA SYLVESTRE AGAINST INSULIN RECEPTOR ON ANTI - DIABETIC EFFECT IN TYPE I AND TYPE II DIABETES(2013) Premchand, S.; Kumarasamy, P.; Sujatha, P.L.; Sureshkannan, S.; TANUVASDiabetes is a metabolic disease characterized by hyperglycemia resulting from defects in insulin secretion and its action. Diabetes can be categorized into three types namely type l diabetes, type ll diabetes and gestational diabetes. The chronic hyperglycemia oi‘ diabetes is associated with long-term damage, dysfunction and failure of various organs mainly the eyes, kidneys, nerves, heart and blood vessels. Generally insulin is used in the treatment of diabetes.ThesisItem Open Access PREPARATION, CHARACTERIZATION AND IN VITRO TRANSFECTION ANALYSIS OF CHITOSAN BASED NANOPARTICLES(TANUVAS, Chennai, 2013) Priya, Y.; Senthilkumar, T.M.A.; Mangalagowri, A.; TANUVASGene therapy is a modern medicine in the present decade to treat many diseases. It is promising a great future in saving many lives in the present world. Based on this, we developed chitosan nanoparticles which can be used as vehicle to transfect the plasmid DNA pEGFP-C1 expressing green fluorescence in the host cells. It was prepared by ionic gelation with polyanions to couple with plasmid and transfect the plasmid into host cells.ThesisItem Open Access ASSESSMENT OF INDIGENOUS ORNAMENTAL FISHERY RESOURCE OF TAMIRAPARANI RIVER(TANUVAS, Chennai, 2013) Kannan, A. Pon Saravana; Venkataramani, V.K.; Francis, T.; Sundaramoorthy, B.; TANUVASA detailed investigation was undertaken to study the indigenous freshwater ornamental fishery resource of Tamiraparani river originating from the Western Ghats of Tamilnadu. The information on the availability and catch details of small indigenous freshwater ornamental fishes in this riverine system is scanty. Further, study on the stock of two commercially important ornamental fish species namely, Etruplus suratensis, Puntius sarana have also not been assessed so far in this riverine systems.ThesisItem Open Access CLINICAL EVALUATION IN SKIN CLOSURE FOR OVARIOHYSTERECTOMY IN DOGS(TANUVAS, Chennai, 2013) Vijay, Karnad Vibhavari; George, Ravi Sundar; Nagarajan, L.; Vairamuthu, S.; TANUVASThe objective of the study was to compare the efficacy of nylon sutures and Octylcyanoacrylate in skin wound closure. The study was carried out in 12 dogs randomly divided into two groups of 6 animals each. The skin wound was closed with nylon sutures in group I and with Octylcyanoacrylate in group ll. Wounds were assessed for healing on postoperative day 3, 7 and l0. Parameters used for wound healing analysis were — erythema, swelling, discharge and infection, dehiscence, pain and overall appearance.ThesisItem Open Access Comparative Evaluation of Radiography and Echocardiography in the Diagnosis of Dilated Cardiomyopathy and Mitral Valve Disease in Dogs(TANUVAS, Chennai, 2013) Dharmendra, Dave Kushal; Gowri, B.; Jayaprakash, R.; Jeyaraja, K.; TANUVASThesisItem Open Access Amplification and Cloning of Manganese Peroxidase Gene from Phanerochaete chrysosporium(TANUVAS, Chennai, 2012) Anjpriya, R.; Kumanan, K.; Vijayarani, K.; TANUVAS