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Professor Jayashankar Telangana State Agricultural University, Hyderabad (Telangana State)

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20181
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Subject: Microbiology × Author: ANJALI, C ×
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    ThesisItemOpen Access
    “GENOTYPING AND PHENOTYPING OF ADVANCED BACKCROSS PROGENIES DERIVED FROM TELLAHAMSA AND MTU1010 RICE VARIETIES FOR BB AND BLAST RESISTANCE’’
    (PROFESSOR JAYASHANKAR TELANGANA STATE AGRICULTURAL UNIVERSITY, 2018) ANJALI, C; DURGARANI, Ch.V
    Bacterial blight (BB) and rice blast are the two most important diseases causing significant yield loss in rice, and they are endemic to several rice growing states of India. In Telangana, the yield loss is very severe due to BB and blast. In order to address the above said issues, the efforts have been made and developed MTU1010 and Tellahamsa introgression lines with bacterial blight (xa13 and Xa21) and blast (Pi54 and Pi1) resistance genes by Institute of Biotechnology, Hyderabad, Rajendranagar. GPP2 was used as a donor for bacterial blight resistance genes (xa13 and Xa21), while NLR145 (Swarnamukhi) was used as donor for blast resistance genes (Pi54 and Pi1). In continuation to a DBT funded research project the present research study entitled “Genotyping and phenotyping of advanced backcross progenies derived from Tellahamsa and MTU1010 rice varieties for BB and Blast resistance” has been designed. DNA bulking method was used to reduce the time, resources and number of samples from 3465 to 99 for DNA isolation without compromising the quality of DNA. Genotyping was carried out in 29 advanced backcross progenies (BC3F4, BC4F5) for the presence of four target genes viz., xa13, Xa21, Pi54 and Pi1 by using gene specific / linked PCR based molecular markers viz., xa13-prom, pTA248, Pi54 MAS and RM224, respectively. The bulk sample analysis of 29 progenies exhibited uniformity for the target genes. 25 Progenies were screened for BB resistance along with resistant donor parent, GPP2; susceptible check, TN1 and recurrent parents, MTU1010 and Tellahamsa. A highly virulent isolate of Xoo (IX-020) from Indian Institute of Rice Research (IIRR) was used for BB screening. Out of 25 progenies, 23 progenies recorded resistance score of “1” where as two progenies were moderately resistant with a score of “3”. These genes were found to be very effective in all the genetic backgrounds (23 out of 25 showed resistance, while 2 were with moderate resistance).The results revealed that xa13 and Xa21 genes individually and together offered high level of resistance compared to recurrent parents. However it is always preferred to use pyramided lines rather than lines with single resistance gene to avoid break down of resistance genes. Twenty five progenies were screened for blast resistance, along with resistant donor parent, NLR145, susceptible check, HR12 and recurrent parents, MTU1010 and Tellahamsa by Principal Scientist (Pathology, ICAR-IIRR) using a highly virulent blast isolate (SPI-04. Six progenies viz., TPL-59, TPL-62, TPL-63, MPL-3, MPL-7 and MPL-8 showed resistance with a score of “3”, while eight progenies viz., TPL-58, TPL-60, TPL-61, TPL-67, TPL-68, TPL-69, MPL-1 and MPL-4 showed moderate resistance to blast with a score of “5”. Pi54 and Pi1 genes individually and together exhibited resistance to moderate resistance in 14 out of 25 lines. Unlike BB resistance genes blast resistance genes showed suscetibilty in 11 progenies (TPL-53, TPL-54, TPL-55, TPL-56, TPL-57, TPL-64, TPL-65, TPL-66, MPL-2, MPL-5 and MPL-9). Towards developing durable blast resistance, it is desirable to introgress additional major gene(s) and QTLs with minor effects. During precise introgression of blast resistance genes from NLR145 with simultaneous recovery of recurrent parent genome (> 96%), several other genes or QTLs responsible for offering blast resistance might be lost from NLR145, which could be one of the reasons for blast suscetibilty in some of the introgressed lines. Agro-morphological evaluation was carried out in 29 advanced backcross lines. Replication wise data was recorded for eight yield and yield contributing characters and RBD analysis was carried out. In case of Tellahamsa derived lines, six progenies (TPL53, TPL-56, TPL-59, TPL-62, TPL-68 and TPL-69) shown significant superiority over Tellahamsa and four lines (TPL-54, TPL-55, TPL-60 and TPL-71) were on par with Tellahamsa for yield. Among 10 MTU1010 derived lines, six lines (MPL-1, MPL-3, MPL-4, MPL-7, MPL-8 and MPL-9) showed significant superiority over MTU1010 for yield. TPL-59, MPL-7 and MPL-8 were found to be promising having yield superiority over parents with BB and blast resistance.