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Kerala Veterinary and Animal Sciences University, Wayanad

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20211
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Subject: Veterinary Surgery and Radiology × Author: ANJANA SATHEESH × Start date: 2020 ×
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    ThesisItemOpen Access
    COMPARATIVE EVALUATION OF DECELLULARISED BOVINE OMENTUM AND POLYIONIC GEL DRESSING FOR HEALING OF FULL THICKNESS BURNS IN RAT MODEL
    (Kerala Veterinary and Animal Sciences University,Pookode, Wayanad, 2021-08-10) ANJANA SATHEESH; Reji Varghese
    Burns are considered as a serious global public health concern. Full thickness extensive burns are often accompanied by inflammatory and immune response, metabolic changes and hypovolemic shock which can lead to multi-organ failure and eventually death. Apart from the physical damage caused to the body. burns also affect the mental well-being and quality of life of the patient due to the excessive scarring and skin contractures. Extensive research is being carried out to replicate different aspects of burn injury, understand the unique pathophysiology as well as to develop novel treatment modalities for managing the burn wounds. Hence, the study was conducted to evaluate the efficiency of decellularized bovine omentum and polyionic gel in burn wound healing and and to compare the efficacy of the above two treatment protocols with silver sulphadiazine, which is the conventional treatment of burn wounds. The study was conducted in 63 adult male Wistar rats with an average body weight of 150g. In all the animals full thickness burn wounds were created on mid dorsal line at thoracolumbar area under general anaesthesia using custom made burn inducing device. The animals were randomly divided into three groups with 21 animals each. In group A, necrosed tissue was excised after 72 h of burn infliction and the wound was grafted with decellularized bovine omentum. In group B, wounds were treated with polyionic gel containing sodium carbonate, sodium chlorite, sodium hydroxide and sodium sulphate. In group C, the wounds were dressed using silver sulphadiazine and was kept as control group. All animals tolerated well with the experiment. There was no sign of systemic affection or wound infection during the observation period. The animals were observed up to 30 days post burn infliction or till complete wound healing. Decellularised omental graft produced fastest healing with highest wound contraction rates and minimal scarring. Through its properties of evoking extensive neovascularisation, Shoplasia and recpithelialisation, grafting of full thickness burn wound helped in increasing the wound contraction rate and reducing the time for wound closure with minimal scarring indicating good quality of healing. Topical therapy using polyionic gel for full thickness wound resulted in better healing compared to the control group. There was significant reduction in healing time when compared tothe control group. The antioxidant properties of the material might have helped in arresting the burn wound progression on the initial days. Anti-inflammatory action and antimicrobial action of the polyionic gel resulted in faster wound healing without any complications. Silver Sulphadiazine, the conventional topical treatment for burn wounds had proved to delay wound healing and produced excessive scarring. Burn wound progression was evident on the initial days. Wound healing was not complete even at the end of observation period. Inflammatory phase of wound healing was prolonged in this group and this could be attributed to one of the reasons for delayed wound healing and also for excessive scarring.