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Sri Venkateswara Veterinary University, Tirupati

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  • ThesisItemOpen Access
    Effect of Salinity and pH on the Growth and Haematology of Pangasianodon hypophthalmus (Sauvage, 1878)
    (SRI VENKATESWARA VETERINARY UNIVERSITY TIRUPATI - 517 502. (A.P.) INDIA, 2020-12) SRINIVASULU SRAVANI, RAMADASU; Balasubramanian, A (MAJOR); Suguna, T; Anupama, R.R
    The present study was carried out to investigate the growth and haematology of Pangasianodon hypophthalmus exposing to various salinities of 0 (Control), 4, 9 and 14 ppt and pH of 6, 7.5 (Control), 9 and 9.5. The experiment was carried out in two components i.e., salinity and pH for a period of 30 days each and 10 Nos. of randomly selected fish were stocked in every experimental tank. The physico-chemical parameters were estimated everyday; whereas, growth and haematological parameters were estimated weekly. In first component, effect of different levels of salinity (0 (C), 4, 9 and 14 ppt) on survival, growth and haematology were studied. The results indicated that P. hypophthalmus could tolerate salinities up to 14 ppt with 100% survival rate throughout the experimental period. However, the growth in terms of length and weight, Specific Growth Rate (SGR) and Protein Efficiency Ratio (PER) decreased with increase in salinity from 0 ppt to 14 ppt owing to utilization of high energy Author : RAMADASU SRINIVASULU SRAVANI Title of the thesis : “Effect of Salinity and pH on the Growth and Haematology of Pangasianodon hypophthalmus (Sauvage, 1878)” Submitted for the Award of degree : Master of Fisheries Science in Fisheries Resource Management Faculty : Faculty of Fisheries Science Department : Department of Fisheries Resource Management Major advisor : Dr. A.BALASUBRAMANIAN University : Sri Venkateswara Veterinary University, Tirupati Year of Submission : 2020 which directly affects the growth of fish in unfavourable environments. In contrary, Feed Conversion Ratio (FCR) raised with increase in salinity and low feed intake was noticed in 14 ppt. Similarly, haematological parameters viz.,Total Erythrocyte Count (TEC), Haemoglobin (Hb) and Haematocrit (Ht) were low in 14 ppt when compared with other salinities of 9 ppt and 4 ppt but Total Leukocyte Count (TLC) was found high in 14 ppt which indicated that the experimental fish were in high stress condition. Mean Corpuscular Volume (MCV) and Mean Corpuscular Haemoglobin Concentration (MCHC) were also varied with changes in the Hb and Ht. After observing the significant (p < 0.05) elevation in haematological parameters of the fish studied in higher salinities, it is concluded that the salinity of 9 ppt is optimum for good growth in P. hypophthalmus. Prior to the actual experiment on effect of pH, lethal studies (LC50) were conducted which resulted in 100% survival of the juveniles up to pH of 9.5. Therefore, the pH levels of 6, 9 and 9.5 were opted with pH 7.5 as control. The experimental results showed decreasing trend in length and weight, SGR and PER from pH 6 to 9.5 compared with control (7.5). However, FCR values were found peak at pH 9.5 followed by 9 and 6 when compared with control. The alterations in growth parameters were due to low feed intake, loss of energy and stress. The mean values of haematological parameters like TEC, Hb, Ht and MCHC showed decreasing trend from pH 6 to 9.5 when compared to control. But TLC values were found maximum at pH 9.5 followed by 9, 6 and 7.5 (C), whereas, the MCV values were found high at pH 9 and least at pH 9.5. Changes in haematological parameters significantly (p<0.05) varied between different pH. The changes in the haematological parameters were due to immunological response, failure of the erythropoeisis and haemopoietic potential.