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University of Agricultural Sciences, Dharwad

The University of Agricultural Sciences, Dharwad was established on October 1, 1986. The University has 5 Colleges, 27 Research Stations, 6 Agriculture Extension Education Centers, 6 Krishi Vigyan Kendras and ATIC. The University has its jurisdiction over 7 districts namely Bagalkot, Belgaum, Bijapur, Dharwad, Gadag, Haveri, and Uttar Kannada in northern Karnataka. Greater diversity exists in soil types, climate, topography cropping and farming situations. The jurisdiction includes dry-farming to heavy rainfall and irrigated area. Important crops of the region include sorghum, cotton, rice, pulses, chilli, sugarcane, groundnut, sunflower, wheat, safflower etc. The region is also known for many horticultural crops. Considerable progress has been registered in the field of education, research and extension from this University.

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  • ThesisItemOpen Access
    Expression and antifungal activity of trichoderma virens ech42 in tobacco
    (UAS, Dharwad, 2007) B.M.Murali; Sumangala Bhat
    Endochitinase is a principal fungal phytopathogen cell wall chitin degrading enzyme utilized to develop transgenic crops resistance to diseases. The full length cDNA coding for endochitinase gene (ech42) was cloned from Trichoderma virens total RNA isolated from induced fungal mycelium. The amplicon obtained through RT-PCR using gene specific primers was cloned into pTZ57R/T vector and confirmed through PCR amplification, restriction analysis, and sequencing. Analysis of sequence has shown 99 per cent homology with the reported endochitinase gene at nucleotide and protein levels. Further, the endochitinase gene sequence was in silico modified and artificially synthesized to overcome codon bias and other undesirable regulatory coding sequences for its improved expression in tobacco. The purified endochitinase was obtained by expressing the modified gene in Escherichia coli with His-tag fusion sequence facilitating Nickel agarose column chromatography. The transgenic tobacco plants with endochitinase gene (ech42g) were analyzed for its expression. Transgenic plants were PCR screened with marker (nptII) and endochitinase gene specific primers. Expression of ech42g at transcription level was confirmed through RT-PCR. Chitinase activity was analyzed through glycol chitin plate assay and reducing sugar estimation across three different growth stages. All the tested transgenic plants showed higher level of chitinase activity compared to untransformed control tobacco plants, and the variation was observed among the progenies of different transgenic lines. Transgenic expression of ech42g tobacco showed resistance against foliar pathogen Alternaria spp. causing leaf spot and soil borne pathogen Sclerotium rolfsii causing root rot.