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University of Agricultural Sciences, Dharwad

The University of Agricultural Sciences, Dharwad was established on October 1, 1986. The University has 5 Colleges, 27 Research Stations, 6 Agriculture Extension Education Centers, 6 Krishi Vigyan Kendras and ATIC. The University has its jurisdiction over 7 districts namely Bagalkot, Belgaum, Bijapur, Dharwad, Gadag, Haveri, and Uttar Kannada in northern Karnataka. Greater diversity exists in soil types, climate, topography cropping and farming situations. The jurisdiction includes dry-farming to heavy rainfall and irrigated area. Important crops of the region include sorghum, cotton, rice, pulses, chilli, sugarcane, groundnut, sunflower, wheat, safflower etc. The region is also known for many horticultural crops. Considerable progress has been registered in the field of education, research and extension from this University.

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20042
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Subject: Biotechnology × Author: Rajkumar ×
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    ThesisItemOpen Access
    DETECTION AND MAPPING OF QTLs FOR CHARCOAL ROT RESISTANCE AND YIELD COMPONENTS IN SORGHUM [Sorghum bicolor (L.) Moench]
    (UNIVERSITY OF AGRICULTURAL SCIENCES GKVK, BANGALORE, 2004) Rajkumar; Fakrudin, B
    "The genetic arclnitecture of cliarcoal rot resistance and drought tolerance through the construction of molecular linkage maps and identification of QTLs along with molecular variability of Macrophomina phaseolina can expectedly hasten the development of charcoal rot and drought tolerant sorghum cultivars. The study consisted of 93 Recombinant Inbred Lines (RILs) derived from IS22380 and E36-1 . Evaluated for charcoal rot incidence and yield traits in rab. 2003. The contrasting behavior of parents for different traits including charcoa rot resistance was responsible for witnessing the wide range of variability foi the characters studied. Analysis of variance revealed that RILs displayed highlv significant differences in their mean performances for all the characters studied. A total of 240 RAPDs and 24 SSR markers were used and of which IE RAPDs and 19 SSRs were found to be polymorphic; all 37 markers genotypec across all RILs in 4 % sequencing gels by silver staining. Genetic linkage maf: was constructed in which 31 markers were assigned to A, B, D, E, F and h linkage maps by simple interval mapping [MAPMAKER/EXP anc MAPMAKER/QTL (1.1b)). QTL analysis indicated two QTLs for length o infection one each on A (AC13 and ATS) and B (AC12 and G16) linkag( groups; five QTLs for percent lodging on A, B, D, and F linkage group: explaining 15.90, 12.10, 20.30 and 10.90 % phenotypic variance respectively. All the ten isolates of Macrophomina phaseolina representing Dharwai and Bijapur regions proved pathogenic to sorghum; molecular profiling usin( RAPD markers indicated the genetic differences among isolates and was specie specific fingerprint to Macrophomina phaseolina was identified. Isolates wer grouped using molecular and virulence data."
  • Thumbnail Image
    ThesisItemOpen Access
    DETECTION AND MAPPING OF QTLs FOR CHARCOAL ROT RESISTANCE AND YIELD COMPONENTS IN SORGHUM [Sorghum bicolor (L.) Moench]
    (UNIVERSITY OF AGRICULTURAL SCIENCES GKVK, BANGALORE, 2004) Rajkumar; Fakrudin, B
    "The genetic arclnitecture of cliarcoal rot resistance and drought tolerance through the construction of molecular linkage maps and identification of QTLs along with molecular variability of Macrophomina phaseolina can expectedly hasten the development of charcoal rot and drought tolerant sorghum cultivars. The study consisted of 93 Recombinant Inbred Lines (RILs) derived from IS22380 and E36-1 . Evaluated for charcoal rot incidence and yield traits in rab. 2003. The contrasting behavior of parents for different traits including charcoa rot resistance was responsible for witnessing the wide range of variability foi the characters studied. Analysis of variance revealed that RILs displayed highlv significant differences in their mean performances for all the characters studied. A total of 240 RAPDs and 24 SSR markers were used and of which IE RAPDs and 19 SSRs were found to be polymorphic; all 37 markers genotypec across all RILs in 4 % sequencing gels by silver staining. Genetic linkage maf: was constructed in which 31 markers were assigned to A, B, D, E, F and h linkage maps by simple interval mapping [MAPMAKER/EXP anc MAPMAKER/QTL (1.1b)). QTL analysis indicated two QTLs for length o infection one each on A (AC13 and ATS) and B (AC12 and G16) linkag( groups; five QTLs for percent lodging on A, B, D, and F linkage group: explaining 15.90, 12.10, 20.30 and 10.90 % phenotypic variance respectively. All the ten isolates of Macrophomina phaseolina representing Dharwai and Bijapur regions proved pathogenic to sorghum; molecular profiling usin( RAPD markers indicated the genetic differences among isolates and was specie specific fingerprint to Macrophomina phaseolina was identified. Isolates wer grouped using molecular and virulence data."