Thumbnail Image

University of Agricultural Sciences, Dharwad

The University of Agricultural Sciences, Dharwad was established on October 1, 1986. The University has 5 Colleges, 27 Research Stations, 6 Agriculture Extension Education Centers, 6 Krishi Vigyan Kendras and ATIC. The University has its jurisdiction over 7 districts namely Bagalkot, Belgaum, Bijapur, Dharwad, Gadag, Haveri, and Uttar Kannada in northern Karnataka. Greater diversity exists in soil types, climate, topography cropping and farming situations. The jurisdiction includes dry-farming to heavy rainfall and irrigated area. Important crops of the region include sorghum, cotton, rice, pulses, chilli, sugarcane, groundnut, sunflower, wheat, safflower etc. The region is also known for many horticultural crops. Considerable progress has been registered in the field of education, research and extension from this University.

Browse

20041
Reset filters
Subject: Biotechnology × Author: Ashajyothi, S S ×
Reset filters

Search Results

Now showing 1 - 1 of 1
  • Thumbnail Image
    ThesisItemOpen Access
    STUDIES ON REGENERATION AND AGROBACTERIUM MEDIATED IN VITRO AND IN PLANTA TRANSFORMATION IN CHILLI (Capsicum annuum L)
    (University of Agricultural Science, Dharwad, 2004) Ashajyothi, S S; Bhat, Sumangala
    "In vitro regeneration potential was studied in two popular local cultivars viz., Byadgi Dabbi and Sankeshwar local. Different explants (Distal half of cotyledon. Basal half of cotyledon. Whole cotyledon, Shoot tip and Hj'pocotyl portion) were cultured on medium with various level of BAP (2 to 12 mg/1) for multiple shoot bud induction. Sankeshwar local was found to give better response compared to Byadgi Dabbi. Highest per cent response was obtained from whole cotyledon cultured on 10 mg/1 BAP. Addition of 2 mg/1 GAs in shoot bud induction medium (10 mg/1 BAP) produced better differentiated shoot buds. Elongation and rooting was observed on MS with 0.1 mg/1 NAA + 0.2 mg/1 BAP and 2 mg/I IBA respectively. Different concentrations and combinations of growth regulators were tried to study callus induction and regeneration from cotyledon and hypocotyl explants. Though calli were induced, regeneration could not be achieved. However, on higher level of lAA with 0.5 mg/1 kinetin cream, compact and small calli were induced under complete darkness and shoots were regenerated after 45 days of exposure to light. For in vitro transformation, Shoot tips were co-cultivated with Agrobacterium stain EHA105 carrying pBinBta construct having cry 1 (Ac) gene. Average of 0.535 per cent PCR positive plants were obtained. simple dipping and vacuum infiltration was carried out at different stages with Agrobacterium culture harbouring pCAMBIA having gus gene or pBinBta having cryl(Ac) gene. Simple dipping of seeds completely inhibited germination. Dipping of seedlings in culture inoculum having different concentration of Triton- X-100 showed expression of gus gene in leaf samples of plants. In case of floral dip, per cent flowers showing GUS expression was highest when treated with culture inoculum having 0.02 per cent Triton-X-100. However, vacuum infiltration of germinating seeds with Agrobacterium having pCAMBIAlSOl showed GUS expression in five per cent plants, while infiltration of seedlings showed expression of gene in ten per cent of plants. Per cent transformation was low (0.5%) when germinating seeds were vacuum infiltrated with Agrobacterium culture having cry 1 (Ac) gene"