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University of Agricultural Sciences, Dharwad

The University of Agricultural Sciences, Dharwad was established on October 1, 1986. The University has 5 Colleges, 27 Research Stations, 6 Agriculture Extension Education Centers, 6 Krishi Vigyan Kendras and ATIC. The University has its jurisdiction over 7 districts namely Bagalkot, Belgaum, Bijapur, Dharwad, Gadag, Haveri, and Uttar Kannada in northern Karnataka. Greater diversity exists in soil types, climate, topography cropping and farming situations. The jurisdiction includes dry-farming to heavy rainfall and irrigated area. Important crops of the region include sorghum, cotton, rice, pulses, chilli, sugarcane, groundnut, sunflower, wheat, safflower etc. The region is also known for many horticultural crops. Considerable progress has been registered in the field of education, research and extension from this University.

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20051
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Subject: Biotechnology × Author: Aseemulla Baig, M M ×
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    ThesisItemOpen Access
    TRANSFORMATION STUDIES IN SUGARCANE (Saccharum officinarum L.)
    (UNIVERSITY OF AGRICULTURAL SCIENCES GKVK, DHARWAD, 2005) Aseemulla Baig, M M; Bhat, Sumangala
    "Importance of Agrobacterium mediated transformation is increasing in sugarcane as means of introducing better and useful traits into many cultivars of economical relevance. As Agrobacterium mediated transformation protocol differ from one plant species to the other and within species, the optimization of transformation methodology requires consideration of several factors that can increase transformation efficiency. Calli derived from leaf and leaf sheaths of sugarcane cultivars Coc-671 and Co-86032 were tested in vitro for kanamycin sensitivity during shoot regeneration and concentration of 25 mg/l was found optimum for the selection of putative transformants. Different factors influencing Agrobacterium mediated transformation have been investigated using Agrobacterium strain EHA-105 that harboured a binary vector pBinBta carrying neomycin phosphotransfesate II and crylAc gene in the T-DNA region and putative transformants selected were confirmed by PCR amplification of npt II gene. Of different factors considered, cocultivation for three days, Agrobacterium cell density of 1.0 OD, 200 ;L;M acetosyringone was found to be better. In addition, use of fresh calli for cocultivation, desiccation of calli for 30 min prior to co-cultivation, co-culturing on solid medium in the absence of 2, 4-D and use of 400 mg/l cystein in infection and co-cultivation medium were found to increase frequency of PCR positive plants. In one experiment, Agrobacterium mediated and biolistic transformation were compared using construct GV 2260 with gus A reporter gene. Per cent transformation was found to be more in biolistic method. In total 21 npt II positive plants were obtained and of which two plants expressed crylAc protein."