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University of Agricultural Sciences, Dharwad

The University of Agricultural Sciences, Dharwad was established on October 1, 1986. The University has 5 Colleges, 27 Research Stations, 6 Agriculture Extension Education Centers, 6 Krishi Vigyan Kendras and ATIC. The University has its jurisdiction over 7 districts namely Bagalkot, Belgaum, Bijapur, Dharwad, Gadag, Haveri, and Uttar Kannada in northern Karnataka. Greater diversity exists in soil types, climate, topography cropping and farming situations. The jurisdiction includes dry-farming to heavy rainfall and irrigated area. Important crops of the region include sorghum, cotton, rice, pulses, chilli, sugarcane, groundnut, sunflower, wheat, safflower etc. The region is also known for many horticultural crops. Considerable progress has been registered in the field of education, research and extension from this University.

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  • ThesisItemOpen Access
    HISTOLOGY AND HISTOCHEMISTRY OF THE ENDOCRINE ORGANS OF THE INDIAN DONKEY (Equus as in us)
    (University of Agricultural Science, Dharwad, 2001) Pawar, Ashok; Ramakrishna, V
    "The present study on the histology and histochemistry of the endocrine organs viz. pineal , Pituitary , thyroid , parathyroid and adrenal gland in the young and adult Indian donkey to know the cytoarchitecture and histochemical characterstics of the cells with their location and functional status. The gross morphological studies revealed the location and position of various endocrine organs in the Indian donkey. Histologically, the pineal gland consisted of pinealocytes, me lanocytes and glial cells in the parenchyma. Th e presence of two types of pinealoc ytes indicate the functional status of the gland. The pituitary gland consisted of a d e n oh yp op h y sis and neurohypophys is. The a d e n o h yp o p h y s is consisted of acidophils, basophils and ch romo p h o be s . Neu ro hyp o ph y s is consisted of pituicytes and neuroglial cells. Herring bodies were noticed in the n ear blood vessels. Thyroid gland mainly consisted of small, me d ium and large follicle Vi/ith blood vessels and parafollicular cells. The presence of small follicles were more in n umb e r and lineu by cuboidal epithelium in young donkey. Large follicles lined by tlat epithelium with loss or no colloid indicatGd ttu? aclivily of the gland. Parathyroid gland consisted, oxyphil,two types pf cliie f cells and parafollicular cells. Th e adrenal gland was consisted of outer cortex, middle g a nglionic and medulla. Epinephrine and norepinephrine cells were noticed in the niedulla. Histochemically. Histochemically the pinealocytes of the pineal, basophils and neurosecretory substances (Herring bodies) o f pituitary, colloid o f tlie thyroid, chief cells and oxyphil cells of parathyroid and zona arcuata and zona reticularis of adrenal gland showed PAS positive reaction. Pinealocytes of pineal, basophils of pituitary, follicular epithelium and colloid o f follicles, chief cells o f parathyroid and ganglionic cells o f the adrenal gland were alcian blue (pH 2.5) positive. No significant difference in the histology and histochemistry between young and adult Indian donkey endocrine glands except pineal and thyroid gland."
  • ThesisItemOpen Access
    CLONING OF ENDOCHITINASE GENE FROM NATIVE ISOLATES OF Trichoderma Sp.
    (UNIVERSITY OF AGRICULTURAL SCIENCES GKVK, BANGALORE, 2004) Anigol, Aishwarya R; Bhat, Sumangala
    "The present study was conducted to screen native fungal isolates for chitinolytic activity and clone the endochitinase gene. Among the fungal isolates (Trichoderma harzianum strains 1,2,3, Trichoderma koningii, Trichoderma virens, Trichoderma viride, Metarrhizium uerrucasia, Bauveria bassiana, Aspergillus oryzae and Nomurea releyi), Trichoderma koningii showed highest hydrolytic activity when screened in Mandels and Reese (1969) salts supplemented with coUoidal chitin as the sole carbon source followed by Aspergillus oryzae. Nomuraea releyi showed least activity. Others were on par with each other. Molecular screening with endochitinase gene specific primers, showed the presence of 260 bp fragment Trichoderma harzianum isolates 1,2,3, Trichoderma koningii and Trichoderma virens but absence in Trichoderma viride and Aspergillus oryzae. Further attempts were made to clone endochitinase gene from Trichoderma species, harzianum, koningii and virens. Desired length of ampUcon was not obtained in Trichoderma koningii Trichoderma harzianum and Trichoderma virens showed desired ampUcons with the designed primers. A 1.7 Kb ampUcon from Trichoderma virens and 1.3 Kb from Trichoderma harzianum were cloned into pTZ57R/T. The clones were confirmed through plasmid minipreparation, PCR amplification, sequencing and homology search. The clones were sequenced and analysed for homology at nucleotide, protein and domains of protein. Trichoderma harzianum clones showed no homology with, the endochitinase genes. Among the 11 clones obtained, pSAV804 was sequenced There was 98% homology at both nucleotide and protein levels wdth the endochitinase genes from Trichoderma spp. The protein was conserved at 85.7%. The primer sequence position in the insert and homology search confirmed the reverse orientation of the insert in the vector. The sequence consisted of two ORFs corresponding to the endochitinase gene one with 274 codons and the other 48 codons. It may be because of occurance of stop codon during PCR. With the available sequence information it can be concluded that the gene is novel."
  • ThesisItemOpen Access
    PCR-RFLP OF MITOCHONDRIAL DNA OF PENINSULAR CARP Labeo fimbriatus (Bloch.)
    (UNIVERSITY OF AGRICULTURAL SCIENCES GKVK, BANGALORE, 2001) Sade, Robert S; Sudharshana, L
    ABSTRACT NOT AVAILABLE
  • ThesisItemOpen Access
    BIOCHEMICAL CHARACTERIZATION OF DWARF COWPEA (Vigna unguiculata (L.) Walp.) MUTANT FOR NITROGEN FIXATION CHARACTERS
    (University of Agricultural Sciences GKVK, Bangalore, 2000) Anjana, G; Thimmaiah, S K
    ABSTRACT NOT AVAILABLE
  • ThesisItemOpen Access
    ISOLATIOH AND CHARACTERIZATION OF LECTIN FROM FIELD BEAN ( Lablab purpureus ) ROOTS
    (University of Agricultural Sciences GKVK, Bangalore, 2002) Narasimha Prasad, K; Podder, Uma S
    ABSTRACT NOT AVAILABLE
  • ThesisItemOpen Access
    POPULATION GENETICS OF PENINSULAR CARP Labeo fimbriatus (Bloch.) USING BIOCHEMICAL MARKERS
    (University of Agricultural Sciences GKVK, Bangalore, 2000) Parimala, P; Sudharshana, L
    ABSTRACT NOT AVAILABLE
  • ThesisItemOpen Access
    COMPARISON OF GEL PROFILES OF SURFACE COMPONENTS OF RHIZOBIUM SPECIFIC AND NON-SPECIFIC TO CHICKPEA (Cicer arietinum L.)
    (University of Agricultural Sciences GKVK, Bangalore, 2001) Aggarwal, Anup; Podder, Uma S
    ABSTRACT NOT AVAILABLE
  • ThesisItemOpen Access
    BIOCHEMICAL STUDIES ON CHICKPEA (Cicer arietinum L.) ROOT LECTIN AND ITS COMPARISON WITH SEED LECTIN
    (University of Agricultural Sciences GKVK, Bangalore, 2002) Jagadhish Kumar, H R; Podder, Uma S
    ABSTRACT NOT AVAILABLE