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University of Agricultural Sciences, Dharwad

The University of Agricultural Sciences, Dharwad was established on October 1, 1986. The University has 5 Colleges, 27 Research Stations, 6 Agriculture Extension Education Centers, 6 Krishi Vigyan Kendras and ATIC. The University has its jurisdiction over 7 districts namely Bagalkot, Belgaum, Bijapur, Dharwad, Gadag, Haveri, and Uttar Kannada in northern Karnataka. Greater diversity exists in soil types, climate, topography cropping and farming situations. The jurisdiction includes dry-farming to heavy rainfall and irrigated area. Important crops of the region include sorghum, cotton, rice, pulses, chilli, sugarcane, groundnut, sunflower, wheat, safflower etc. The region is also known for many horticultural crops. Considerable progress has been registered in the field of education, research and extension from this University.

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20041
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Subject: Biochemistry × Author: Anigol, Aishwarya R ×
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    ThesisItemOpen Access
    CLONING OF ENDOCHITINASE GENE FROM NATIVE ISOLATES OF Trichoderma Sp.
    (UNIVERSITY OF AGRICULTURAL SCIENCES GKVK, BANGALORE, 2004) Anigol, Aishwarya R; Bhat, Sumangala
    "The present study was conducted to screen native fungal isolates for chitinolytic activity and clone the endochitinase gene. Among the fungal isolates (Trichoderma harzianum strains 1,2,3, Trichoderma koningii, Trichoderma virens, Trichoderma viride, Metarrhizium uerrucasia, Bauveria bassiana, Aspergillus oryzae and Nomurea releyi), Trichoderma koningii showed highest hydrolytic activity when screened in Mandels and Reese (1969) salts supplemented with coUoidal chitin as the sole carbon source followed by Aspergillus oryzae. Nomuraea releyi showed least activity. Others were on par with each other. Molecular screening with endochitinase gene specific primers, showed the presence of 260 bp fragment Trichoderma harzianum isolates 1,2,3, Trichoderma koningii and Trichoderma virens but absence in Trichoderma viride and Aspergillus oryzae. Further attempts were made to clone endochitinase gene from Trichoderma species, harzianum, koningii and virens. Desired length of ampUcon was not obtained in Trichoderma koningii Trichoderma harzianum and Trichoderma virens showed desired ampUcons with the designed primers. A 1.7 Kb ampUcon from Trichoderma virens and 1.3 Kb from Trichoderma harzianum were cloned into pTZ57R/T. The clones were confirmed through plasmid minipreparation, PCR amplification, sequencing and homology search. The clones were sequenced and analysed for homology at nucleotide, protein and domains of protein. Trichoderma harzianum clones showed no homology with, the endochitinase genes. Among the 11 clones obtained, pSAV804 was sequenced There was 98% homology at both nucleotide and protein levels wdth the endochitinase genes from Trichoderma spp. The protein was conserved at 85.7%. The primer sequence position in the insert and homology search confirmed the reverse orientation of the insert in the vector. The sequence consisted of two ORFs corresponding to the endochitinase gene one with 274 codons and the other 48 codons. It may be because of occurance of stop codon during PCR. With the available sequence information it can be concluded that the gene is novel."