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University of Agricultural Sciences, Dharwad
The University of Agricultural Sciences, Dharwad was established on October 1, 1986.
The University has 5 Colleges, 27 Research Stations, 6 Agriculture Extension Education Centers, 6 Krishi Vigyan Kendras and ATIC. The University has its jurisdiction over 7 districts namely Bagalkot, Belgaum, Bijapur, Dharwad, Gadag, Haveri, and Uttar Kannada in northern Karnataka. Greater diversity exists in soil types, climate, topography cropping and farming situations. The jurisdiction includes dry-farming to heavy rainfall and irrigated area. Important crops of the region include sorghum, cotton, rice, pulses, chilli, sugarcane, groundnut, sunflower, wheat, safflower etc. The region is also known for many horticultural crops.
Considerable progress has been registered in the field of education, research and extension from this University.
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ThesisItem Open Access Identification of Single Chain Fragment Variable Clones Expressing Monoclonal Antibody Against Potato Leaf Roll Virus Coat Protein(University of Agricultural Science, Dharwad, 2017-09) Shivaranjini C.; Moger, NarayanThesisItem Open Access Molecular Interaction Between Rhizobacteria and Root Pathogens in Groundnut (Arachis hypogea L.) and Brinjal (Solanum melongena L.)(University of Agricultural Science, Dharwad, 2017-11) Roopa K.P.; Krishnaraj, P.U.ThesisItem Open Access Molecular Interaction of Arabidopsis-Rhizobacteria for Induced Systemic Tolerance Under Water Deficit Condition(University of Agricultural Science, Dharwad, 2017-07) Basavaarya, B.R.; Krishnaraj, P.U.Root colonization by actinobacteria induces systemic drought tolerance in Arabidopsis thaliana. Actinobacterial cultures were taken from culture repository of Department of Biotechnology, UAS, Dharwad on February, 2015. Experiment was conducted in vitro at the Department of Biotechnology. Collected isolates were screened under water deficit condition using Poly Ethylene Glycol 6000 (PEG) and tested for the production of Indole Acetic Acid (IAA), a plant growth promoting harmone. Three actinobacterial cultures were tested for their rhizospheric interaction with Arabidopsis thaliana plants under water deficit conditions. The isolate AUDT 651 promoted good root (10.68 cm) and shoot (11.38 cm) growth better than AUDT 545 and AUDT 605. Illumina RNA seq was performed to identify differentially expressed genes from plants inoculated with or without actinobacteria under drought stressed conditions and normal growth conditions. Only genes having a log2-fold change ≥ 2.0 or ≤ -2.0 and an adjusted p-value < 0.05, were included in present analysis of differential gene expression. While genes involved in metabolism, transport and abiotic stress tolerance were up regulated in actinobacterial inoculated conditions, stress responsive genes like scavenging enzymes (catalase, superoxide dismutase, peroxidase), LEA protein and ethylene-responsive transcription factor were down regulated. Transcripts of photosynthetic genes (cytochrome b6-f complex subunit, photosystem II reaction center protein), and transporter genes (detoxification protein, chloroplastic) were uniquely expressed in actinobacterial colonized plants. These data show how gene expression in plants in water deficit condition can be influenced by microbial colonization leading to plant protection, induced by the presence of actinobacteria in the rhizosphere.ThesisItem Open Access Study of Mungbean Yellow Mosaic Virus (Mymv) Resistance in Mungbean (Vigna radiata L. Wilczek) and Urdbean (Vigna mungo L. Hepper) Employing Nbs-Lrr and in-Silico Based Markers(University of Agricultural Science, Dharwad, 2017-11) Jyothi N.; Patil, B.R.The present investigation was carried out at the Institute of Agriculture Biotechnology (IABT), Dharwad, during 2015- 16 with an objective to study the mungbean yellow mosaic virus (MYMV) resistance in urdbean and mungbean employing NBS-LRR and in-silico based markers. 107 and 13 genotypes of mungbean and urdbean were screened for MYMV disease at GPB botanical garden in summer 2015. Genetic variability, correlation and molecular diversity were studied. Sixteen genotypes of mungbean were found to be resistance to MYMV. Among the urdbean genotypes only three genotypes, viz., UTTARA, IPU-02-43 and DU3 exhibited moderate resistance reaction to MYMV. Markers MTB 99, RGA1-TG, VuRS02F16V and SI-INV were specific to MYMV. The molecular diversity analysis revealed that markers used in the study distinguished the mungbean and urdbean genotypes by forming separate clusters. High GCV, PCV, higher heritability and high genetic advance over the mean were recorded for number of branches, number of bunches, number of pods per plant and total seed yield indicated the presence of additive gene action. So these traits can be improved through simple direct selection. The total seed yield exhibited highly significant and positive correlation with pods per plant, pod length, seeds per pod and hundred seed weight at both phenotypic and genotypic level among urdbean genotypes which indicates indirect selection of these traits will improve the seed yield. The mungbean genotypes viz., HUM1, SML 134, HUM 12 and urdbean genotypes, viz., IPU-02-43, UTTARA which showed resistance to MYMV can be utilized as parents in developing disease resistant varieties. The four putative markers identified can be used to differentiate the resistant and susceptible genotypes among mungbean genotypes and they could also be further validated using mapping populations.ThesisItem Open Access Induced Mutagenesis in the Introgression Lines to Improve Resistance to Foliar Diseases and Productivity in Groundnut (Arachis hypogaea L.)(University of Agricultural Science, Dharwad, 2017-11) Joshi, Pushpesh; Bhat, Ramesh S.Present investigation was carried out to develop and evaluate induced mutants for superior productivity traits and resistance to foliar diseases in groundnut. M0 seeds of IL 3 and IL 4 were mutagenized with gamma rays (200 Gy and 300 Gy) and sodium azide (2 mM and 3 mM). The M1 seeds were sown during post-rainy season 2015 at IABT garden (E115), MARS, UAS, Dharwad. In M1 population, increasing dose of gamma rays led to reduction in germination and increase in lethality. But the increasing dose of sodium azide resulted in increased germination. The M2 generation was raised during the rainy season of 2016. Both IL 3 and IL 4 recorded the score of 5 for ELS and 3 for LLS. ELS score ranged from 3 to 6 for both IL 3 and IL 4 derived mutants. LLS score ranged from 2 to 4 in IL 3 derived mutants and 1 to 4 in IL 4 derived mutants. In IL 3 and IL 4, the mutation frequency increased with increase in concentration of the mutagens and mutagenic effectiveness decreased with increase in dose of the mutagens individually and in combination. IL 3, IL 4 and their selected mutants were subjected to ddRAD-Seq to discover SNPs and copy number variations (CNVs). Number of SNPs varied from 2 to 12 in IL 3 derived mutants and 1 to 9 in IL 4 derived mutants. Total 86 M3 lines were evaluated during post-rainy season 2016. Genotypes showed significant difference for majority of traits except for sound mature kernel weight percentage. All the M3 lines and parents showed resistant type of allele at all the foliar disease resistance-linked marker loci. Four IL 3 derived M3 lines were found superior for productivity over parent and can be tested under multi-locations for variety development.ThesisItem Open Access Identification of Single Chain Fragment Variable Monoclonal Antibody Against Coat Protein of Tomato Leaf Curl Virus Using Phage Display Technology(University of Agricultural Science, Dharwad, 2017-06) Honnesh S.H.; Patil, M.S.An investigation was carried out to identify single chain fragment variable monoclonal antibody against Tomato leaf curl virus (ToLCV) coat protein using phage display technology, conducted at department of biotechnology, University of Agricultural Sciences, Dharwad during 2015-16. ToLCV is a major Geminivirus which causes serious loss to tomato production in tropical and subtropical regions of the world. The most commonly used diagnostic tool for ToLCV detection is immunological assays, which is dependent on the availability of highly specific antibody to differentiate the viruses. Production of antibody using phage display technology needs pure protein therefore, ToLCV/CP was bacterially expressed. A 786 bp PCR product containing coat protein coding region of ToLCV was amplified using ToLCV CPF and ToLCV CPR primers and the amplified product was cloned into the pTZ57R/T and further subcloned in to the pQE30. The transformed clones were confirmed through PCR and sequencing. The coat protein was expressed using 1 mM IPTG. A band of 31 kDa on the gel confirmed that coat protein was really fused to the His-tag. Further, the coat protein was purified using His-tag purification kit. Four rounds of biopanning was performed by coating purified ToLCV /CP in to the immunotube using Tomlinson library. The fourth biopan reading (1.37) showed higher binding specificity to ToLCV/CP. These were subsequently used for scFv monoclone for ToLCV/CP. Finally, the randomly selected scFv clones were screened with ELISA. ELISA reading showed that one clone had higher binding affinity to ToLCV/CP. The sequencing of the clone showed 80% similarity with the scFv antibody gene (DQ375454.1). The selected clone is highly specific to ToLCV/CP as there was no cross reaction with PRSV and groundnut GBNV. Further, the sensitivity test results imply that the developed scFv antibody can detect ToLCV coat protein at the concentration of 20 g/ml.ThesisItem Open Access Genetic analysis and validation of markers linked to nitrogen use efficiency and Yield components in bread wheat(University of Agricultural Science, Dharwad, 2017-08) Ranjitha K.M.; Biradar, Suma S.The present investigation was carried out for genetic analysis and validation of markers linked to NUE and yield components in bread wheat. Six parents were crossed in half diallel fashion and all the 15 diallel crosses obtained along with the six parents were evaluated in hydroponic culture for root and shoot parameters under Hoagland half NO3 media in the Department of Biotechnology, UAS, Dharwad, during 2015-16. The same 15 diallel crosses and six parents were evaluated in field under 50 per cent N condition for yield attributes and NUE related traits at AIRCP wheat, MARS, UAS, Dharwad during 2016-17. Results of combining ability analysis showed the predominance of non-additive gene action in controlling all characters under both the condition. The parent UAS323 for root characters and the two parents GW322, 2 WYCYT34 for yield and NUE related traits were found to be the best general combiners. The crosses GW322 × K9107 and 2 WYCYT34 × C306 for root and shoot parameters and the crosses namely, GW322 × C306, 2 WYCYT34 × C306 and K9107 × C306 for NUE and yield attributes were found to be the best specific combiners. Out of 15 diallel crosses, three populations GW322 × K9107, 2 WYCYT34 × C306 and 2 WYCYT34 × UAS323 were selected and advanced to F2 population. Genetic variability, correlation and transgressive segregants analysis indicated that these three populations could be used for future breeding programs to improve NUE. However, the F2 population of the cross 2 WYCYT34 × C306 found to be more promising. Validation by using 44 SSR markers in the three F2 populations showed that the potential SSR marker m081 showing significant association with most of the agronomic traits and the marker wmc720 associated with NUE in particular hence it can be used for improvement of NUE in wheat.ThesisItem Open Access Association Analysis of Charcoal Rot Disease and Yield Component Traits in Sorghum Minicore Germplasm with EST-SSR and Allelic Variation in SbRGA114(University of Agricultural Science, Dharwad, 2016-03) Harsha Kumar, B.N.; Bhat, SumangalaEvaluation of sorghum minicore consisting of 242 accessions during rabi season (2014-15) at RARS, Vijayapura revealed significant variations both at phenotypic and genotypic level for charcoal rot disease and yield component traits. High values of PCV, GCV and heritability coupled with GA was recorded for percent lodging, plant height, panicle exertion, earhead length, earhead width, number of leaves per plant, 100 seed weight, seed yield per plant. SbRGA114 based association for charcoal rot component traits in a set of 30 sorghum minicore accessions revealed nucleotide changes at seven positions with minor allele frequency(MAF) of >5%. Six significant associations (P<0.05) were observed for nucleotide changes at five positions (SbRGA114 86, SbRGA114 102, SbRGA114 944, SbRGA114 1278, SbRGA114 110) with phenotypic variation ranging from 2.9- 7.5%. Thereby SbRGA114 can be used for candidate gene based association analysis and if validated, will be useful for improving Rabi sorghum for charcoal rot resistance through molecular breeding. Total of 31 polymorphic EST-SSR markers which were developed and mapped at IABT UAS-Dharwad was used for association analysis. Population structure was assigned based on molecular data in Structure v. 2.0 software, while association analysis was done by Tassel program using the Q matrix. Six markers (Xiabt 210, Xiabt 527, Xiabt 301, Xiabt 37, Xiabt 77, Xiabt 81) for charcoal rot component traits and thirteen (Xiabt 82, Xiabt 85, Xiabt 47, Xiabt 30, Xiabt 241, Xiabt 457, Xiabt 275, Xiabt 397, Xiabt 378, Xiabt 380, Xiabt 445, Xiabt 81 and Xiabt 527) markers for yield component traits were stable at two locations viz., MARS Dharwad and RARS, Vijayapura. Also in the present study, previously identified flanking markers for charcoal rot disease (locus Xiabt 275 at RARS Vijayapura location) and yield related QTLs (Xiabt 81 and Xiabt 527 both MARS Dharwad and RARS Vijayapura locations) were validated. Therefore these markers can be utilised in marker assisted selection in charcoal rot disease resistance and yield improvement breeding programs.ThesisItem Open Access BIOCHEMICAL STUDIES ON RESISTANCE TO Fusarium WILT IN PIGEONPEA (Cajanus cajan (L.) Millisp.) SEEDLINGS(University of Agricultural Sciences, Dharwad, 2000) SRINIVAS, H; THIMMAIAH, S KABSTRACT NOT AVAILABLE
