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University of Agricultural Sciences, Dharwad

The University of Agricultural Sciences, Dharwad was established on October 1, 1986. The University has 5 Colleges, 27 Research Stations, 6 Agriculture Extension Education Centers, 6 Krishi Vigyan Kendras and ATIC. The University has its jurisdiction over 7 districts namely Bagalkot, Belgaum, Bijapur, Dharwad, Gadag, Haveri, and Uttar Kannada in northern Karnataka. Greater diversity exists in soil types, climate, topography cropping and farming situations. The jurisdiction includes dry-farming to heavy rainfall and irrigated area. Important crops of the region include sorghum, cotton, rice, pulses, chilli, sugarcane, groundnut, sunflower, wheat, safflower etc. The region is also known for many horticultural crops. Considerable progress has been registered in the field of education, research and extension from this University.

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  • ThesisItemOpen Access
    Development and Evaluation of Ds Tagged Mutants in Sorghum
    (UAS, Dharwad, 2013) Amrish H. Antre; Remesh Bhat
    Maize Dissociator (Ds)-mediated insertional inactivation tagging and activation tagging were attempted in M 35-1, a popular rabi variety of Sorghum bicolor L. (Moench) for its gene discovery and functional genomics. Six and five transgenic events produced from pUR224NA and pNU435, respectively were used as the Ds starter lines for insertional inactivation tagging. Five Ds events obtained with pUbiDs were used for activation tagging. Five events of iAc plants obtained with pKU352NA were used as the source of transposase. Ds starter lines with single copy of the launch pad (T-DNA/Ds) were selected by segregation analysis and used for crossing with iAc plants following artificial emasculation and hand pollination. The seeds set on the female plant were harvested and sown in the green house. The plants showing the presence of both Ds and iAc as tested by various PCR were regarded as true F1s. The seeds borne on the true F1s were harvested to raise the F2 generation. Among the F2 plants, those with transposed Ds (excised out of launch pad and reinserted within the genome) and without iAc were identified by various PCR as Ds tagged stable mutants. In total, five and three insertionally inactivated mutants (IIM) were obtained from the launch pads of pUR224NA and pNU435, respectively. In addition, three activation tagged mutants (ATM) were recovered from the launch pad of pUbiDs. Majority of the mutants showed unlinked Ds transposition as they did not carry the empty launch pad (TDNA without Ds). Six mutants (IIM2, IIM3, IIM6, IIM7, ATM1 and ATM3) with unlinked transposition showed Ds insertion within genic regions, indicating tagging of six different genes of sorghum. Preliminary phenotyping of the Ds tagged mutants (without ascertaining their zygosity) under green house for various morphological traits did not reveal any gross morphological changes when compared to M 35-1 (wild type).