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Bihar Agricultural University, Sabour

Bihar Agricultural University, Sabour established on 5th August, 2010 is a basic and strategic institution supporting more than 500 researchers and educationist towards imparting education at graduate and post graduate level, conducting basic, strategic, applied and adaptive research activities, ensuring effective transfer of technologies and capacity building of farmers and extension personnel. The university has 6 colleges (5 Agriculture and 1 Horticulture) and 12 research stations spread in 3 agro-ecological zones of Bihar. The University also has 21 KVKS established in 20 of the 25 districts falling under the jurisdiction of the University. The degree programmes of the university and its colleges have been accredited by ICAR in 2015-16. The university is also an ISO 9000:2008 certified organisation with International standard operating protocols for maintaining highest standards in teaching, research, extension and training.VisionThe Bihar Agricultural University was established with the objective of improving quality of life of people of state especially famers constituting more than two third of the population. Having set ultimate goal of benefitting society at large, the university intends to achieve it by imparting word-class need based agricultural education, research, extension and public service.

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20171
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Subject: Plant Pathology × Author: Karn, Monika ×
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    ThesisItemOpen Access
    Deciphering the role of Nightshade (Solanum nigrum) in epidemiology of Tomato leaf curl virus infecting Tomato (Solanum lycopersicum)
    (Department of Plant Pathology, BAU, Sabour, 2017-07) Karn, Monika; Ansar, Mohammad
    Tomato (Solanum lycopersicum L.) is the world’s fourth important vegetable crop with an annual production of 162 million metric tons. At present India ranks second in overall production worldwide. The crop is severely hampered by various biotic constraints, among them viruses play an important role. In whitefly (Bemisia tabaci) transmitted geminiviruses, Tomato leaf curl virus (ToLCV) affect tomato cultivation to a huge extent. The disease may cause up to 90 per cent or more reduction in fruit yield due to its devastating nature. Weed plants are well known reservoir of begomoviruses which act as major factor in epidemiology of the disease in different crops. Considering the economic importance of crop and potentiality of associated virus the present investigation was undertaken to study the prevalence of ToLCV disease in Bhagalpur locality along with associated Solanum nigrum (nightshade) weed. The study covered molecular detection and characterization of virus in tomato and nightshade weed, reciprocal transmission experiments and temporal dynamics of viral incidence. The survey for viral infection in tomato and nightshade in rabi season (2016-17) revealed ToLCV was present in almost locations of Bhagalpur district. Disease incidence ranged from 25 to 38 per cent in tomato and 11 to 22 per cent in nightshade. Population dynamics of whitefly during survey revealed 1 to 3 individuals/plant on tomato and 0.3 to 3 on nightshade plant. In molecular detection of virus carried out through PCR assay, with specific primer pairs (Deng-F/R, ToLCNDV-F/R, and ToLCNDV-AF/AR). PCR amplified product expected size of 957 bp band in gel electrophoresis. The product having CP gene was clonned and sequenced through outsourcing. After procurement of sequence, full CP gene sequence were checked through ORF finder which revealed a single open reading frame with 771 nucleotide encoding 256 amino acid. The full sequence was submitted to GenBank under the accession number KY112754. Sequence data was subjected to multiple sequence alignments and phylogram was constructed using CLUSTAL W software. Based on the CP gene homology of the virus isolates infecting nightshade regarded as an isolates of ToLCNDV and is designated as ToLCNDV:SN (94-96 % homology with isolates of ToLCNDV). In artificial transmission experiment of ToLCV, average rate of 16.6 per cent recorded from infected tomato to nightshade plant (seedling, 6-leaf and flowering stage). Back transmissibility of ToLCV from infected nightshade to healthy tomato plant was found to be 12.21 per cent. In temporal dynamic studies of leaf curl in tomato and nightshade incidence progressively increases upto middle February. A similar trend was observed in case of nightshade plant but the appearance was noticed after symptom expression in tomato. A positive correlation was found in tomato and nightshade leaf curl incidence under the study. The study covers a detail exploration of associated nightshade weed in epidemiology of tomato leaf curl virus. The result seems to helpful in growers community in order to manage the disease by destroying weeds.