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Bihar Agricultural University, Sabour

Bihar Agricultural University, Sabour established on 5th August, 2010 is a basic and strategic institution supporting more than 500 researchers and educationist towards imparting education at graduate and post graduate level, conducting basic, strategic, applied and adaptive research activities, ensuring effective transfer of technologies and capacity building of farmers and extension personnel. The university has 6 colleges (5 Agriculture and 1 Horticulture) and 12 research stations spread in 3 agro-ecological zones of Bihar. The University also has 21 KVKS established in 20 of the 25 districts falling under the jurisdiction of the University. The degree programmes of the university and its colleges have been accredited by ICAR in 2015-16. The university is also an ISO 9000:2008 certified organisation with International standard operating protocols for maintaining highest standards in teaching, research, extension and training.VisionThe Bihar Agricultural University was established with the objective of improving quality of life of people of state especially famers constituting more than two third of the population. Having set ultimate goal of benefitting society at large, the university intends to achieve it by imparting word-class need based agricultural education, research, extension and public service.

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20211
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Subject: Biotechnology and molecular Biology × Author: Kumari, Seema ×
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    ThesisItemOpen Access
    Genetic basis of waterlogging stress tolerance in pigeonpea (Cajanus cajan L.)
    (Department of Molecular Biology & Genetics Engineering, BAU, Sabour, 2021-07) Kumari, Seema; Thakur, Dharamsheela
    Waterlogging stress is major abiotic stress having detrimental effects on pigeonpea productivity worldwide. This study was conducted with 13 pigeonpea genotypes for identification of waterlogging stress tolerant genotypes and to identify morpho-physiological traits and molecular marker linked to water logging stress tolerance. Waterlogging treatment was given at early vegetative stage (30 days after sowing) for 6 days by placing pots in cemented tank filled with water 5 cm above the soil. Plant survival was recorded at 2, 4 and 6 days of waterlogging treatment, and after 5 and 10 days of drainage. Different morphological and biochemical parameters including seed coat colour, root length, shoot length, number of root branches, chlorophyll content, peroxidase (POX), Catalase, Super oxide dismutase (SOD) and alcoholic dehydrogenase (ADH) activity along with total soluble sugar (TSS) content were studied under control and waterlogged condition. Survival data revealed that the genotypes ICPL 20241, ICPL 20092, AK-13B and ICPL 84023 showed high level of tolerance towards waterlogging stress whereas genotype ICPL 87 and ICP 7035 showed least tolerance. Pigeonpea genotype ICPL 20092 having cream coloured seed showed high level of tolerance whereas ICP 7035 with dark brown seed coat showed susceptibility towards waterlogging stress so no correlation could be established between dark seed coat colour and plant survival. A significant decline in root length, primary root branches and chlorophyll content was observed in all genotypes under water logging stress with greater level of decrease in susceptible genotypes than tolerant genotypes. POX, SOD and ADH activity increased significantly as a consequence of waterlogging stress, and was higher in tolerant than susceptible genotypes. Catalase activity increased in response to water logging treatment in some tolerant as well as susceptible genotypes but increase could not be correlated with waterlogging tolerance. Increase in TSS content was observed in response to water logging in root of tolerant genotypes whereas TSS got decreased in susceptible genotype. Based on results of the present investigation pigeonpea genotypes ICPL 20241, ICPL 20092, AK-13B and ICPL 84023 have been identified as tolerant genotypes and change in root length, root branches, chlorophyll, TSS content, POX, SOD and ADH activity as major morpho-physiological markers for waterlogging stress tolerance study. Five genic SSRs were found to be polymorphic and grouped nineteen pigeonpea into three major clusters and five sub-clusters. Four genic SSRs were found to be transferable to chickpea. ADH promoter primer targeting CAAT box mutant region showed amplification in tolerant pigeonpea genotypes and no amplification in susceptible genotypes. This primer could be used as functional marker in identification of waterlogging stress tolerant pigeonpea genotype.