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Bihar Agricultural University, Sabour

Bihar Agricultural University, Sabour established on 5th August, 2010 is a basic and strategic institution supporting more than 500 researchers and educationist towards imparting education at graduate and post graduate level, conducting basic, strategic, applied and adaptive research activities, ensuring effective transfer of technologies and capacity building of farmers and extension personnel. The university has 6 colleges (5 Agriculture and 1 Horticulture) and 12 research stations spread in 3 agro-ecological zones of Bihar. The University also has 21 KVKS established in 20 of the 25 districts falling under the jurisdiction of the University. The degree programmes of the university and its colleges have been accredited by ICAR in 2015-16. The university is also an ISO 9000:2008 certified organisation with International standard operating protocols for maintaining highest standards in teaching, research, extension and training.VisionThe Bihar Agricultural University was established with the objective of improving quality of life of people of state especially famers constituting more than two third of the population. Having set ultimate goal of benefitting society at large, the university intends to achieve it by imparting word-class need based agricultural education, research, extension and public service.

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20211
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Subject: Biotechnology and molecular Biology × Author: Kumari, Priyanka ×
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    ThesisItemOpen Access
    Knockdown of movement protein (MP) of Potato Leaf Roll Virus (PLRV) using RNA interference technology
    (Department of Molecular Biology & Biotechnology, BAU, Sabour, 2021-03) Kumari, Priyanka; Ranjan, Tushar
    Viruses cause many severe plant diseases, resulting in immense losses of crop yield worldwide. Potato crop is severely affected by various biotic stresses and among which viruses play a significant contribution in terms of huge loss in crop yield worldwide including India. Potato is affected by deadly viruses especially more potential infection via seed tubers due to the vegetative reproduction of the crop. More than 40 viruses and viroids hampering the cultivation of potato across the globe. The crop is infected by more than 30 RNA viruses, out of which 13 are mainly transmitted by aphids. Potato leaf roll virus (PLRV), belongs to genus Polerovirus and family Luteoviridae, is widely spreaded potato virus worldwide and responsible for more than 20 million tonnes yield loss (up to 90 %) globally. PLRV is only transmitted by aphids, namely, Myzus persicae. It is widely multiplied in the phloem tissue and the symptoms of disease reflect this position. Because potato is a vegetatively propagated crop, once it gets infected with viruses, they can easily disseminate in the progeny tubers. These viruses are found in single or most of the time as a mixed infection within the potato crops. Tubers used for planting in next season can harbor latent viruses that subsequently reduce emergence, plant vigor and yield. All daughter tubers produced by infected mother tubers (secondary infection) will become infected via systemic translocation of the virus during growth. Therefore, developing novel approaches to control plant viruses is crucial to meet the demands of a growing world population. Recently, RNA interference (RNAi) has been widely used to develop virus-resistant plants. Once genome replication and assembly of virion particles is completed inside the host plant, viral genomes spread cell-to-cell through plasmodesmata (PD) by interacting with the virus-encoded movement protein (MP). MPs help in the enlargement of PD pore size and active transport of the viral nucleic acid or mature viral particle as well into the adjacent cell, thereby allowing local and systemic spread of viruses in plants. We used the RNAi approach to suppress MP gene expression, which in turn prevented potato leafroll virus (PLRV) systemic infection in Solanum tuberosum cv. Khufri Ashoka. Potato plants agroinfiltrated with MP siRNA constructs exhibited no rolling symptoms upon PLRV infection, indicating that the silencing of MP gene expression is an efficient method for generating PLRV-resistant potato plants. Further, we identified novel ATPase motifs in MP for the first time that may be involved in viral nucleic acid binding and their translocation through plasmodesmata during viral maturation process. We also showed that the putative ATP binding motifs viz., walker A, walker B, sensor motif, arginine finger I and II together forms a fantastic binding site for substrate ATP. Intriguingly, in silico data also fetched DNA binding domain in MP. Based on the above findings, we proposed a hypothetical model for transportation of mature virion particles or their naked genome across the PD. Overall, our findings provide a robust technology to generate PLRV-resistant potato plants, which can be extended to other species. Phylogenetic analysis of MP from different families of plant viruses showed that Luteoviridae has ancestral existence, which later on diversed into multiple variants during course of evolution. These findings will further complement in developing biochemical and structural approaches to study the plant virus transportation mechanisms through PD. Moreover, this approach contributes to the study of genome translocation mechanisms of plant viruses.