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Anand Agricultural University, Anand

Anand Agricultural University (AAU) was established in 2004 at Anand with the support of the Government of Gujarat, Act No.(Guj 5 of 2004) dated April 29, 2004. Caved out of the erstwhile Gujarat Agricultural University (GAU), the dream institution of Sardar Vallabhbhai Patel and Dr. K. M. Munshi, the AAU was set up to provide support to the farming community in three facets namely education, research and extension activities in Agriculture, Horticulture Engineering, product Processing and Home Science. At present there seven Colleges, seventeen Research Centers and six Extension Education Institute working in nine districts of Gujarat namely Ahmedabad, Anand, Dahod, Kheda, Panchmahal, Vadodara, Mahisagar, Botad and Chhotaudepur AAU's activities have expanded to span newer commodity sectors such as soil health card, bio-diesel, medicinal plants apart from the mandatory ones like rice, maize, tobacco, vegetable crops, fruit crops, forage crops, animal breeding, nutrition and dairy products etc. the core of AAU's operating philosophy however, continues to create the partnership between the rural people and committed academic as the basic for sustainable rural development. In pursuing its various programmes AAU's overall mission is to promote sustainable growth and economic independence in rural society. AAU aims to do this through education, research and extension education. Thus, AAU works towards the empowerment of the farmers.

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20124
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Subject: Plant Biotechnology × End date: 2012 × Start date: 2012 × Thesis Type: M.Sc ×
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Now showing 1 - 4 of 4
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    ThesisItemOpen Access
    “Identification and validation of internal control genes for quantitative gene expression studies in pearl millet under biotic stress (downy mildew infection) and abiotic stress (salt stress
    (Anand Agricultural University, Anand, 2012) POONAM D. KANANI; Dr. Y. M. Shukla
    Pearl millet (Pennisetum glauccum (L.) R. Br.) is an annual, cross pollinating, monocot crop belonging to the family Poaceae and sub family Penicedae. It is the sixth most important cereal of the world and has critical value as food security in some of the world’s hottest and driest cultivated areas of Africa and Asia. However, the crop faces constraints to the productivity which includes the prevalence of different biotic and abiotic stresses. The present work was carried out with an aim to identify and select endogenous genes for pearlmillet under biotic stress (downy mildew) and abiotic stress (salinity) and validating constitutively expressed genes in pearlmillet through RT-PCR and analyzed it through geNorm for best stable reference gene
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    ThesisItemOpen Access
    VALIDATION OF ENDOGENOUS GENES IN RICE (Oryza sativa L.) UNDER DROUGHT AND SALT STRESS”
    (Anand Agricultural University, Anand, 2012) ANUCHITHRA V.L.; Dr. Subhash N.
    The use of suitable and stable reference genes for normalization is an important and unavoidable step during gene expression studies in relative quantification using Real Time PCR. Validation of endogenous genes in rice (Oryza sativa L.) under drought and salt stress was carried out with an aim to identify most suitable reference genes for the normalization of gene expression data in rice. After four days of drought and salinity stress the resistant cultivars showed higher RWC as compared to the susceptible cultivars. The drought susceptible cultivar, GR 3 showed higher leaf rolling index (LRI) as compared to the resistant cultivar AAUDR 1. Based on the visual symptoms of salt damage the tolerant cultivar Dandi scored 3 in the Standard Evaluation System (SES) and susceptible cultivar GR 11 scored 7, indicating tolerance and susceptible reactions, respectively. cDNA preparation was carried out from total RNA of rice seedlings
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    ThesisItemOpen Access
    DIFFERENTIAL GENE EXPRESSION STUDY FOR HEAT, DROUGHT AND COMBINE HEAT AND DROUGHT IN WHEAT (Triticum aestivum L.) GENOTYPE”
    (Anand Agricultural University, Anand, 2012) RAFALIYA RUTULKUMAR VALLABHBHAI; Dr. Y. M. Shuklaa
    Wheat (Triticum spp.) is self pollinated cereal crop, belonging to the family Poaceae (grasses) and is the most important and universally grown of all food grains having its origin in south western Asia. It is estimated that one third of the world’s population depend on wheat for their nutritional requirements. Abiotic stress is the primary reason for farm production loss worldwide, causing average yield reductions of more than 50%, in wheat (Triticum aestivum). The present investigation was carried out with an aim to study the candidate genes responsible for resistance against heat and drought in wheat and comparison of gene expression between control and stress tissues with in susceptible and resistant genotypes through RT-PCR. Genotypes differing in degree of sensitivity to drought, heat, combine heat and drought stress selected were GW11 and GW273. The study was conducted with objectives for screening of Hsps genes under heat, drought and combine heat & drought stress and also to study the differential
  • Thumbnail Image
    ThesisItemOpen Access
    “DIFFERENTIAL GENE EXPRESSION STUDY FOR HEAT, DROUGHT AND COMBINE HEAT AND DROUGHT IN WHEAT (Triticum aestivum L.) GENOTYPE”
    (Anand Agricultural University, Anand, 2012) RAFALIYA, RUTULKUMAR VALLABHBHAI; Dr. Y. M. Shukla
    Wheat (Triticum spp.) is self pollinated cereal crop, belonging to the family Poaceae (grasses) and is the most important and universally grown of all food grains having its origin in south western Asia. It is estimated that one third of the world’s population depend on wheat for their nutritional requirements. Abiotic stress is the primary reason for farm production loss worldwide, causing average yield reductions of more than 50%, in wheat (Triticum aestivum). The present investigation was carried out with an aim to study the candidate genes responsible for resistance against heat and drought in wheat and comparison of gene expression between control and stress tissues with in susceptible and resistant genotypes through RT-PCR. Genotypes differing in degree of sensitivity to drought, heat, combine heat and drought stress selected were GW11 and GW273. The study was conducted with objectives for screening of Hsps genes under heat, drought and combine heat & drought stress and also to study the differential ii expression of Hsp genes in wheat through real time PCR and bioinformatics tools. Physiological parameters suggested that root length, shoot length and relative water content significantly decreased in stress conditions as compared to control condition in both the genotypes. However, between the genotypes GW273 had high effect various stresses as compared to GW11 for all the physiological parameters. Total RNA concentration ranged from 332.56-630.00 ng/μl. From total RNA, cDNA synthesis was carried out to study differential gene expression. The genes selected were Hsp70, Hsp26.5, Hsp17.3, Hsp16.9, Hsp23.6 and Hsp 101b. Gene expression study revealed that in drought stress Hsp70, Hsp26.5, Hsp16.9 and Hsp17.3 had higher regulation in genotype GW11. Hsp17.3 and Hsp26.5 showed the maximum expression as compared to Hsp70 and Hsp 16.9 in GW11. In response to heat stress Hsp26.5, Hsp16.9 and Hsp17.3 showed upregulation in genotype GW11 where Hsp17.3 reported the maximum gene expression. The study further revealed all six Hsp genes except Hsp101b were influenced by drought and heat stress. Hsp26.5 and Hsp17.3 were highly upregulated by 6.4 and 5 fold as compare to GW273. Overall gene expression study suggested that Hsp 17.3, Hsp26.5 were upregulated in drought, heat and combine drought-heat in wheat genotypes differing in degree of susceptibility to drought, heat, and combine heat and drought.