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Anand Agricultural University, Anand

Anand Agricultural University (AAU) was established in 2004 at Anand with the support of the Government of Gujarat, Act No.(Guj 5 of 2004) dated April 29, 2004. Caved out of the erstwhile Gujarat Agricultural University (GAU), the dream institution of Sardar Vallabhbhai Patel and Dr. K. M. Munshi, the AAU was set up to provide support to the farming community in three facets namely education, research and extension activities in Agriculture, Horticulture Engineering, product Processing and Home Science. At present there seven Colleges, seventeen Research Centers and six Extension Education Institute working in nine districts of Gujarat namely Ahmedabad, Anand, Dahod, Kheda, Panchmahal, Vadodara, Mahisagar, Botad and Chhotaudepur AAU's activities have expanded to span newer commodity sectors such as soil health card, bio-diesel, medicinal plants apart from the mandatory ones like rice, maize, tobacco, vegetable crops, fruit crops, forage crops, animal breeding, nutrition and dairy products etc. the core of AAU's operating philosophy however, continues to create the partnership between the rural people and committed academic as the basic for sustainable rural development. In pursuing its various programmes AAU's overall mission is to promote sustainable growth and economic independence in rural society. AAU aims to do this through education, research and extension education. Thus, AAU works towards the empowerment of the farmers.

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Subject: Animal Genetics and Breeding × End date: 2004 × Start date: 2004 × Type: Thesis × Thesis Type: Ph.D ×
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    ThesisItemOpen Access
    MOLECULAR GENETIC CHARACTERIZATION OF BANTAM, BANTAMISED WHITE LEGHORN, AND WHITE LEGHORN CHICKENS USING PCR-RAPD, PCR-RFLP & MICROSATELLITE TECHNIQUES
    (AAU, Anand, 2004) Pipalia, Devendrakumar L.; Solanki, J. V.
    The molecular genetic techniques RAPD, PCR-RFLP and microsatellite markers studies were used for investigation of genetic composition and genetic distances among BNT, BWLH and WLH genetic groups of chicken at molecular level. Out of 13 RAPD primers, 9 primers gave amplifications and amongst them 3 primers (BG-1, PLANTAGO-01 and PLANTAGO-06) showed variation between pooled DNA samples from each sex of BNT, BWLH and WLH groups. The BG-1 produced 4 to 7 scorable bands from sexwise pooled DNA samples within 550 to 1550 bp range and amplified approximately 1235 bp female specific band and 625 & 550 bp fragments specific to BWLH and WLH. The band sharing (BS) value was highest between BWLH and BNT group (0.4454) as compared to BWLH and WLH group (0.3880). The PLANTAGO-01 produced 7 to 8 scorable bands from sexwise pooled DNA samples within 724 to 2038 bp range and amplified approximately 1014 bp size fragment specific to BWLH and WLH group. While 724 bp fragment was only found in BNT group. The PLANTAGO-06 primer produced 5 to 8 scorable bands from sexwise pooled DNA samples within 696 to 2530 bp range and amplified approximately 790 and 832 bp fragments specific to BNT group. These primers, PLANTAGO-01 and 06, showed higher BS values between BWLH and WLH (0.6235 and 0.5235) as compared to BWLH and BNT groups (0.5346 and 0.4637). Phylogenetic tree using data revealed that distance between BWLH was less with WLH than BNT group. The PCR products amplified by cGH1 and cGH2 primers specific to chicken growth hormone gene showed size variation as compared to literature i.e. 1216 bp instead of 1163 bp (53 bp difference) for cGHI and 770 bp instead of 756 bp (14 bp difference) for cGH2 region. PCR-RFLP of growth hormone showed restriction enzyme (RE) sites at 628 and 1074 bp at cGHI/Sac / locus and RE sites at 373 and 529 bp at cGH2//Wsp / locus. The new RE sites found at 628 bp for cGHI/ Sac I locus and at 529 bp for cGH2/Msp / locus. The exact size, sequence and position of restriction sites can be more precisely known only by sequencing. For cGHI/Sac / locus, alleles A2 and A3 were not observed in BNT and WLH groups, while only allele A3 was absent in BWLH group. The allele A was found in highest frequency in BNT (0.5357) and WLH (0.5968) groups. While allele A1 was at highest frequency in BWLH (0.7766) group. The allele A2 was only found in BWLH group. It may happen due to insertion of particular restriction site (628 bp) at the time of gametic recombination. The WLH population significantly (P<0.05) deviated from genetic equilibrium at this locus. For cGH2IMsp I locus, the allele frequencies for A1 and A3 alleles were found intermediate in BNT genetic group. For BWLH group, A1 allele showed higher frequency (0.8878), while WLH group showed lack of polymorphism with only A1 allele present. This may be due to mutation or deletion of restriction site at 373 bp in WLH. BNT and BWLH genetic groups were in genetic equilibrium (P<0.01). The phylogenetic trees constructed from cGH1 and cGH2 motifs showed that WLH was at equidistance from BWLH and BNT. The five microsatellite markers i.e. ADL-102, ADL-136, ADL-158, ADL-171 and ADL-172 have shown presence of genetic polymorphism in all genetic groups. The number of alleles ranged from 2 to 6. The marker ADL- 171 was found to be highly polymorphic than other four microsatellite markers. BNT and WLH genetic groups deviated significantly (P<0.01) from genetic equilibrium at ADL-102 locus. All three genetic groups deviated significantly (P<0.01) from genetic equilibrium at ADL-136 locus and ADL-158. The genotypic frequencies of BWLH and WLH populations significantly deviated (P<0.01) from equilibrium at ADL-171 locus. The genetic groups BNT and BWLH were in genetic equilibrium while genetic group WLH significantly (P<0.01) deviated from equilibrium at ADL-172. The heterozygosity for five microsatellite markers ranged from 0.48 for ADL-102 to 0.78 for ADL-171. The PIC values ranged from 0.463 for ADL-102 to 0.683 for ADL-171. The microsatellite marker ADL-171 was more polymorphic and informative than other four microsatellite markers. A consensus tree obtained by bootstrapping multiple data set analysis from all 5 microsatellite markers revealed that WLH and BWLH were forming one cluster in cent percent cases, where as BNT branched out separately from the cluster. The statistical analysis revealed non-significant effect of genotypes at cGH1/Sac / and cGH2/Wsp / locus on BW-8, BW-20, BW-40, TEN-40, EW- 40 and TFC-40 traits. The statistical analysis revealed that various genotypes of all 5 microsatellite markers exerted no significant effect on different production traits under study except for BW-8 in WLH at Adl-102; BW-40 in BNT at ADL- 171; and BW-20, BW-40, TEN-40 and EW-40 in BNT at ADL-172.