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Subject: Plant Physiology ×

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    ThesisItemOpen Access
    Morphogenetic study in bamboo (Bambusa bambos (L.) Voss) for large scale production of planting material
    (Department of Plant Physiology, BAU, Sabour, 2021-01) Kumari, Baby; Pal, Awadhesh Kumar
    Bambusa bambos (L.) Voss, popularly known as Indian Thorny Bamboo is in high demand due to its multipurpose importance. It is cultivated for building and raw material for pulp and paper industry. Being the fastest growing woody plant, it has important role in repairing damaged and degraded soil outside forests. Availability of planting materials is a constraint, as the natural propagation methods have limitation. Tissue culture offers rapid and reliable method to produce planting materials on large scale in lesser time. The current study was taken up with an objective to optimize an efficient system of micropropagation using nodal segments. Choice of explants and different media formulations were standardized for shoot induction, shoot multiplication, rooting and hardening. Nodal segments of 3.0 mm girth and 3.0 cm length beneath the node induced 4.5 shoots per explants in liquid MS basal media supplemented with 3.0 mg/l BAP and 2.0 mg/l TDZ. Shoot multiplication with 20.8 shoots per clump was obtained liquid MS media supplemented with 6.0 mg/l BAP, but the length of shoots originated went on decreasing with the increase in BAP concentrations. Among various concentrations of NAA and BAP, 9.0 mg/l NAA alone gave 18.7 roots per explants. Among the IBA and BAP hormonal combinations, IBA at 20.0 mg/l gave a maximum of 18.4 roots per explants. Total number of roots originated per explant increased significantly with the increase in concentrations of auxins. But within a particular concentration of auxins, the number of roots decreased significantly with the increase in the concentrations of BAP, particularly at the higher concentrations of auxins. NAA at 9.0 mg/l and IBA at 20.0mg/l were at par in terms of root regeneration. In the media containing both NAA and IBA was associated with necrosis, which increased with the concentration of auxins, while within a particular auxins concentration; it decreased with the increase in BAP concentration. Four different potting mixtures including coco-peat, soil, sand and vermi-compost, did not show significant difference, but the days of emergence of new shoots was lesser in coco-peat: soil: sand: vermi-compost (1:1:1:1:1) potting mixture (23.9 days). The optimized procedure may effectively be used for large scale planting material production of Bambusa bambos (L.) Voss.