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Open Access
In-vitro cloning of commercial cultivars of Gerbera (Gerbera jamesonii Bolus)
(Department of Floriculture and Landscaping, OUAT, Bhubaneswar, 2021-10-18) Sharma, Ruchi; Beura, Sashikala; Palai, S.K.; Tripathy, P.; Jagadev, P.N.
The experiments for the thesis entitled “In-vitro cloning of commercial cultivars of Gerbera (Gerbera jamesonii Bolus)” were conducted at Ph. D. Lab, Biotechnology-Cum-Tissue Culture Centre, Department of Floriculture and Landscaping, College of Agriculture, OUAT, Bhubaneswar during the year 2016-17 and 2017-18. The objectives of the study were standardization of surface sterilant and timing for sterilization of explants, effect of plant bio regulators on callus formation, effect of plant bio regulators on shoot proliferation, effect of plant bio regulators on root formation and standardization of media mixture for hardening of three commercially important cultivars viz. Intense, Balance and Goliath. In these experiments quarter capitulum (3-4 days immature buds) was used as explant. The basal media used was MS media and it has been supplemented with different combinations of plant bio regulators for various treatments. Each treatment had 3 replications and each replication had 10 cultures. Therefore, each treatment had 30 cultures. Recorded data for different parameters during different stages of growth were statistically analysed using standard CRD. The result revealed that the explants surface sterilized with 0.1% HgCl2 for 7 min. followed by 0.5% NaOCl for 2 min. significantly resulted in minimum rate of infection i.e. 6.33% and 4.33% with high percentage of survival of cultures i.e. 84.67% and 84.33% in cv. Intense and Goliath respectively. Explants of cv. Balance when surface sterilized with 0.1% HgCl2 for 6 min followed by 0.5% NaOCl for 2 min significantly resulted in minimum rate of infection i.e. 5.33% and high percentage of survival of cultures (88.67%). Early callus induction (16.97 days), early callus proliferation (27.83 days) and maximum callus spread (2.5 cm2 ) in cv. Intense were significantly observed in MS media fortified with 2.5 ppm BAP and 0.5 ppm IAA. In cv. Balance MS media supplemented with 2.5 ppm BAP and 1.0 ppm IAA resulted in early callus induction (19 days), whereas early callus proliferation (30.53 days) and maximum callus spread (2.40 cm2 ) was reported when MS media was supplemented with 2.0 ppm BAP and 0.5 ppm IAA. In cv. Goliath earliness in callus induction (18.67 days) was observed with MS media fortified with 2.5 ppm BAP and 0.5 ppm IAA but early callus proliferation (30.33 days) and maximum callus spread (2.41 cm2 ) was recorded with MS media supplemented with 2.5 ppm BAP and 1.0 ppm IAA. MS media fortified with 4.0 ppm BAP, 1.0 ppm IAA and 100 mg/l ADS significantly resulted in the earliness to shoot initiation i.e. 8.89 and 8.33 days, maximum number of multiple shoots per calli mass i.e. 5.71 and 4.57, maximum shoot length i.e. 5.78cm and 5.72cm in cv. Intense and Goliath respectively and for cv. Balance earliness in shoot initiation (9 days) and maximum shoot length i.e. 5.63 cm was observed with MS media supplied with 4.0 ppm BAP, 0.5 ppm IAA and 100 mg/l ADS, but number of shoots per calli mass was recorded maximum with 3.5 ppm BAP, 1.0 ppm IAA and 100 mg/l ADS. In culturing of shoots and sub culturing 1 and 2 for multiplication and shoot proliferation of the cultures, probable number of multiple shoots was significantly maximum (1468.64 and 1619.68) with MS media fortified with 2 ppm 2ip in cv. Intense and Goliath respectively. Whereas in cv. Balance, MS media fortified with 0.5 ppm TDZ significantly enhanced the maximum probable number of shoots (1497.76) produced. Half MS media fortified with 1.0 ppm IAA was observed significantly suitable for early root initiation within 8.33 days and also enhanced the percentage of cultures producing roots with 91.00% in cv. Intense. Whereas in cv. Balance early root initiation (12.67 days) was achieved with half MS media fortified 0.5 ppm IAA and maximum percentage of culture producing roots (91.33%) with ½ MS media supplemented with 1.0 ppm IAA. In cv. Goliath, ½ MS media fortified with 1.0 ppm IAA significant resulted in early root initiation (13.67 days) with percentage of cultures producing roots (89.00%), but maximum cultures producing root (91.67%) was recorded with full MS media fortified with 1.0 ppm IAA. For hardening and acclimatization of these in vitro produced plantlets they were planted in treatment having potting mixture of Soil, Sand, FYM and Cocopeat in different combination .In cultivars Intense, Balance and Goliath better establishment of plantlets and maximum survival percentage of 98.73%, 100% and 98.63% respectively were significantly recorded with potting mixture of Soil, Sand, FYM and Cocopeat in ratio ½ : 1 : 1: 2.
Open Access
Effect of IBA, Aspirin and natural plant extracts on rooting of Chrysanthemum (Dendranthema grandiflora L.) cv. Flirt
(Department of Floriculture and Landscaping, OUAT, Bhubaneswar, 2021) Sahoo, Tosali Trishna; Maharana, Kaberi; Beura, Sashikala; Palai, Sidhartha Kumar; Dash, Abhiram
The present investigation entitled “Effect of IBA, Aspirin and Natural plant extracts on Rooting of Chrysanthemum (Dendranthema grandiflora L.) cv. Flirt” was carried out in form of pro-tray experiment under polyhouse of the Department of Floriculture and Landscaping, located in the premises of College of Agriculture, O.U.A.T., Bhubaneswar during August – September 2021 with the following objectives: (i) To study the effect of IBA on rooting of cuttings of chrysanthemum, (ii) To study the effect of Aspirin on rooting of cuttings of chrysanthemum and (iii) To study the effect of Natural plant extracts on rooting of cuttings of chrysanthemum. The research study was conducted as per the Completely Randomized Design (CRD) with 8 treatments and 3 replications. The 8 treatments were T1 (control), T2 (IBA 750 ppm), T3 (Aspirin 40mg/l of distilled water), T4 (IBA 750 ppm + Aspirin 40mg/l of distilled water), T5 (Diluted alcoholic leaf extract of Ipomoea aquatica), T6 (20 % water-soluble garlic cloves extract), T7 (Gel from Aloe vera leaves) and T8 (Diluted alcoholic leaf extract of Ivy gourd). The terminal cuttings of chrysanthemum variety, ‘Flirt’ were collected and subjected to different treatment solutions and planted in pro-trays with sand as the rooting medium. Different observations of rooting parameters were recorded at 10, 15 and 20 DAP. The results obtained in the experiment revealed that there was significant effect among the treatments. The treatment T4 i.e., IBA 750 ppm + Aspirin 40mg/l of distilled water proved to be the best treatment for rooting in Chrysanthemum cuttings in comparison to other treatments as well as control, since it exhibited the best results on cuttings, viz., minimum number of days to callus formation (2.40), least number of days for root initiation (6.83), highest number of roots per cutting (15.10 at 10 DAP, 33.93 at 15 DAP, 45.83 at 20 DAP), highest number of secondary roots per cutting at 20 DAP (6.17), maximum length of longest root per cutting (9.15 mm at 10 DAP, 19.58 mm at 15 DAP, 28.90 mm at 20 DAP), maximum fresh weight of roots per cutting (0.23 gm at 15 DAP, 0.34 gm at 20 DAP), lowest number of senescenced leaves per cutting (0.33 at 10 DAP, 0.03 at 20 DAP) and highest rooting percentage in cuttings (96.67 %) as compared to T1 i.e., Control (2.42 days for callus formation, 7.20 days for root initiation, 6.07 number of roots per cutting at 10 DAP, 7.63 number of roots per cutting at 15 DAP, 16.07 number of roots per cutting at 20 DAP, 3.57 number of secondary roots per cutting at 20 DAP, 4.20 mm length of longest root per cutting at 10 DAP, 8.52 mm length of longest root per cutting at 15 DAP, 12.55 mm length of longest root per cutting at 20 DAP, 0.05 gm of fresh weight of roots per cutting at 15 DAP, 0.12 gm of fresh weight of roots per cutting at 20 DAP, 0.63 number of senescenced leaves per cutting at 10 DAP, 0.13 number of senescenced leaves per cutting at 20 DAP and 80 % rooting in cuttings. Similarly, T7 (Gel from Aloe vera leaves) and T6 (20 % water-soluble garlic cloves extract) also recorded best results respectively next to T4 (IBA 750 ppm + Aspirin 40mg/l of distilled water). Therefore, the conclusion from the research work drawn was that naturally available plant extracts and products are organic, non-toxic, readily available at low cost and contain phytohormones and active compounds which stimulate rooting in cuttings, prevents infections as well as support plant growth. Hence, they can be successfully used as effective alternatives of synthetic rooting hormones that are expensive but also toxic to plants and environment at high concentrations. So, the present study opens up many opportunities for further research in future for use of plant extracts and natural products in commercial propagation of floricultural crops.
Open Access
Effect of variable doses of nitrogen and phosphorus on gladiolus variety candyman
(Department of Floriculture and Landscaping, OUAT, Bhubaneswar, 2021) Rahithya, Sanda; Nath, Manas Ranjan; Beura, Sashikala; Palai, Sidhartha Kumar; Mohanty, S.
The present investigation entitled “Effect of variable doses of nitrogen and phosphorus on gladiolus variety candyman” was conducted in College of Agriculture, Odisha University of Agriculture and Technology, Bhubaneswar, during the period of October 2019 to March 2020. The experiment was laid out in Randomized block design, Replicated three times with 12 treatments each and control. Treatments comprised of different doses and split applications of nitrogen and phosphorus and a constant dose of potassium. Sources of fertilizers are urea, single super phosphate and muriate of potash. Among all the treatments vegetative parameters were found maximum in treatment T7 (30grams of nitrogen and 30grams phosphorus applied twice) maximum plant height (66.87 cm), Number of leaves (7.01) leaf width (3.5 cm), leaf length (48.27cm) and plant spread (48.27 cm). Results of reproductive parameters are found maximum in treatment T7 (30grams of nitrogen and 30grams phosphorus applied twice) spike length (127.59 cm), spike thickness(8.47mm), rachis length(42.73 cm), number of florets per spike(11.80), second floret diameter(10.61cm), flowering duration (11.11 days), vase life (5.56 days) and minimum days taken for spike initiation(60.73 days), flower bud initiation(4.67 days) and first floret to second floret opening(2 days). Results of corm parameters in Treatment T7 (30grams of nitrogen and 30grams of phosphorus applied twice) recorded maximum number of corms per plant (1.78), fresh weight of corm (61.13 gm), diameter of corm (5.46 cm), number of cormels (14.22) per plant, fresh weight of cormel (1.64 gm) and diameter of cormel (1.08 cm). Therefore, from the above given results it may be concluded that treatment T7 (30g of nitrogen and 30g phosphorus applied twice) gave the best results with respect to vegetative, reproductive and corm parameters of gladiolus variety candyman.
Open Access
Effect of growth retardants on growth and flowering of garden sunflower (Helianthus debilis Nutt.)
(Department of Floriculture and Landscaping, OUAT, Bhubaneswar, 2021) Thabbannavar, Anuradha Ravasaheb; Pandey, Geeta; Beura, Sashikala; Palai, Sidhartha Kumar; Panda, Rajendra Kumar
An evaluation of “Effect of growth retardants on growth and flowering of garden sunflower (Helianthus debilis Nutt.)” was conducted at the Department of Floriculture and Landscaping, College of Agriculture, Odisha University of Agriculture and Technology, Bhubaneswar, Odisha during January, 2021 to May, 2021. The research was performed to study the effect of four growth retardants viz., cycocel (CCC), daminozide (alar), maleic hydrazide (MH) and paclobutrazol (cultar) on growth and flowering of garden sunflower. CCC, MH and daminozide were applied at 500 ppm, 1000 ppm, 1500 ppm and 2000 ppm, respectively while, the concentration of paclobutrazol was 50 ppm, 100 ppm, 150 ppm and 200 ppm, respectively. The study was undertaken as pot experiment following completely randomised design (CRD) with seventeen treatments replicated thrice. The finding of the study reported that the foliar application of different growth retardants at various concentrations significantly influenced all the vegetative, flowering and post-harvest parameters except number of ray florets per flower in garden sunflower. Daminozide @ 1000 ppm is most effective in reduction of plant height. Foliar application of cycocel @ 2000 ppm produced highest number of secondary branches with maximum plant spread in both East-West and North-South directions. Application of cycocel @ 2000 ppm also resulted in enhancing the floral parameters such as earliest bud initiation, earliest bud showing colour, earliest flower opening and maximum number of flowers per plant with smaller flower diameter. Plants sprayed with MH @1000 ppm produced greater number of primary branches whereas, maximum stem girth and highest number of leaves were observed under treatment of MH @ 1500 ppm. Among post-harvest parameters, it was recorded that fresh weight, dry weight, shelf-life and vase life of flower were maximum under treatment applied with MH @1000 ppm. However, same treatment reported delay in bud initiation, bud showing colour as well as flower opening. Influence of various concentrations of different growth retardants was significantly varied in most of the growth, flowering and post- harvest parameters except number of ray florets per flower which was found to be non-significant. Influence of various concentrations of different growth retardants when applied to garden sunflower showed varied vegetative and floral characteristics, which in turn, significantly improved the plant aesthetics.
Open Access
Effect of Growing container, planting media and nutrients on growth and flowering of Gaillardia (Gaillardia pulchella Foug.)
(Department of Floriculture and Landscaping, OUAT, Bhubaneswar, 2021) Aleesha, Shaik; Maharana, Kaberi; Beura, Sashikala; Tripathy, Lilymoony; Dash, Abhiram
An investigation entitled “Effect of containers, planting media and nutrients on growth and flowering of Gaillardia (Gaillardia pulchella Foug.)” was conducted in the Department of Floriculture and Landscaping, College of Agriculture, Odisha University of Agriculture and Technology, Bhubaneswar during the year 2021 with the following objectives-(i) To study the effect of growing containers on growth and flowering of Gaillardia (ii) To study the effect of media on growth and flowering of Gaillardia (iii) To study the effect of source of nutrients on growth and flowering of Gaillardia. One month old Gaillardia pulchella seedlings were grown in two different types of containers viz. C1 ( clay pots size of 25cm ) and C2 (polybags size of 15 × 15 × 30cm )filled with three types of media leaving 2.5-3cm from the rim of pots and polybags viz.M1( garden soil ), M2( garden soil + sand @ 1:1 v/v ) and M3 ( garden soil + sand + cocopeat @ 1:1:1 v/v ) and three sources of nutrients viz.N1( FYM @ 10g/plant ), N2( vermicompost @ 5g/plant ) and N3( NPK @ 5 : 2.5 : 2.5g/plant ) applied at 30 and 60 days after transplanting. There were 18 treatments with 3 replications and the experiment was laid out in factorial CRD. The materials and methods were followed as per standard procedure. The biometric observations were recorded at 15, 60, 90 and 120 days after transplanting w.r.t plant height, number of leaves, number of branches, leaf area, stem girth, plant spread, days taken for first bud appearance, days taken for bud to ray floret opening, number of flowers and flower diameter. The result obtained indicated that the vegetative characters recorded maximum values with C2 × M3 × N3 viz. plant height (30.86cm), leaf area ( 6.92cm2 ) at 120DAT and number of branches/plant (4.86) at 30DAT . whereas the number of leaves/plant (63.37) at 60DAT was recorded for C2 × M3 × N2 and plant spread (453.96cm2 ) was recorded for C1× M2× N1 and stem girth (4.63cm) at 90DAT was recorded for C1 × M1 × N2. As regards to the floral characters, earliest flowering (51.40 DAT), shortest time to full bloom (4.96 days), more number of flowers/plant (92.06) largest diameter of flowers (6.03cm) was recorded in C2 × M3 × N3. Whereas largest diameter of flowers (6.03cm) was recorded in C2 × M3× N3. Thus, it was concluded that by using polybags as growing containers with a potting mixture of garden soil + sand + cocopeat (1:1:1 v/v) and nutrients (NPK @ 10g/plant) is best for growth and flowering of Gaillardia pulchella under Bhubaneswar conditions.
Open Access
Effect of plant growth regulators on growth and flowering of gomphrena (Gomphrena globosa L.)
(Department of Floriculture and Landscaping, OUAT, Bhubaneswar, 2021) Pravallika, Ronanki Durga; Pandey, Geeta; Beura, Sashikala; Tripathy, Pradyumna; Palai, S.K.
An experiment entitled “Effect of plant growth regulators on growth and flowering of gomphrena (Gomphrena globosa L.)” was carried out at the Department of Floriculture and Landscaping, College of Agriculture, Odisha University of Agriculture and Technology, Bhubaneswar, Odisha during March 2021 to July 2021. The trial was conducted to study the effect of Gibberellic acid (viz., 50 ppm,100 ppm and 150 ppm), Napthalene acetic acid (viz., 100 ppm,150 ppm and 200 ppm) and Maleic hydrazide (viz., 500 ppm, 1000 ppm and 1500 ppm) on two cultivars of gomphrena i.e., SG-9 and SG-27. The study was conducted as a pot culture trial following Factorial Completely Randomized Design (FCRD) with ten treatments, replicated thrice in both the cultivars. The result of the study revealed that the two cultivars of gomphrena performed satisfactorily with respect to several parameters related to growth and flowering, though they exhibited variation among themselves with respect to these parameters. Cultivar SG-27 produced maximum plant height and leaf area. However, it produced minimum plant spread, number of branches, number of leaves and stem girth. The floral parameters such as days to first flowering and 50 per cent flowering were earliest in cv. SG-27. The post harvest parameters such as vase life, fresh weight and dry weight of harvested flower were also recorded maximum in cv. SG-27. Cultivar SG-9 produced plant with minimum plant height and leaf area whereas it also produced plants with maximum plant spread, stem girth, number of primary branches, number of secondary branches and number of leaves. Among floral parameters, cv. SG-9 recorded more duration of flowering, number of flowers and higher flower yield per plant whereas in case of post harvest parameters maximum flower diameter and shelf life were found. Among the treatments, application of GA3@150 ppm recorded the best results with respect to plant height, plant spread, number of primary branches, number of secondary branches, number of leaves and leaf area. While, MH@1500ppm produced dwarf and compact plants with small leaves and higher stem girth. NAA @ 200 ppm resulted in early days to first flowering and 50 per cent flowering along with maximum flowering duration whereas GA3 @ 150 ppm resulted in maximum number of blooms and highest flower yield per plant. However, control resulted in delayed days to first flowering and 50 per cent flowering, minimum flowering duration, flower numbers and least yield. Among, post harvest parameters GA3@150ppm showed higher results in fresh weight of flower, dry weight of flower, flower diameter, vase life and shelf life. While, MH@1500ppm recorded lower fresh weight of flower, dry weight of flower, flower diameter and control recorded lower shelf life and vase life. Interaction of cultivar with treatment was found to be significant for most of the parameters except plant spread, stem girth, fresh weight and dry weight of flower which was found to be non-significant.
Open Access
Assessment of African marigold (Tagetes erecta L.) cultivars suitable for commercial cultivation during summer season under coastal plain zone of Odisha
(Department of Floriculture and Landscaping, OUAT, Bhubaneswar, 2021) Chhattar, Ashutosh; Palai, S.K.; Beura, S.; Nath, M.R.; Panda, N.
The present investigation entitled, “Assessment of African marigold (Tagetes erecta L.) cultivars suitable for commercial cultivation during summer season under coastal plain zone of Odisha.” was conducted at experimental plots of All India Co-ordinated Research Project on Floriculture, Biotechnology-cum-tissue culture Laboratory, Department of Floriculture& Landscaping, College of Agriculture, Odisha University of Agriculture & Technology, Bhubaneswar, Odisha, India during 2020-21. The experiment was laid out with nine treatments and three replication following RBD. Thenine African marigold cultivars chosen for the experiment are, Arka Alankar, Arka Bangara, Arka Agni, Pusa Basanti Gainda, BB-1, BM-1, BM-2, BM-3 and BRMG-113. The genotypes were evaluated for 10 parameters including, plant height, plant spread (N-S), plant spread (E-W), days to 505 flowering, days to first flower bud appearance, flowering duration , number of flowers per plant, diameter of flower, flower weight, loose flower yield per plant were studied. All the character pertinent to vegetative growth and flowering varied significantly among the marigold cultivars evaluated.Maximum plant height (70.27 cm) and longest flowering duration (83.67 days) were observed in cultivar Arka Alankar. Maximum plant spread (55.18 cm) was observed in the cultivar Pusa Basanti Gainda. Least number of days to 50% flowering (69.67 days), maximum individual flower weight (6.27g) and maximum flower diameter (6.7 cm) were observed in cultivar Arka Bangra. Earliest flower bud appearance was observed in BM-3(54.00 days). Maximum number of flower per plant (135.22) and loose flower yield per plant (0.77 kg) was observed in cultivar BRMG-113. It was observed that the loose flower yield of African marigold cultivar BMRG 113 was maximumin coastal plain zone of Odisha during summer season.
Open Access
Varietal performance of tuberose (Polianthes tuberosa L.) in Bhubaneswar condition
(Department of Floriculture and Landscaping, OUAT, Bhubaneswar, 2021) Nayak, Sambit Sourabh; Nath, Manas Ranjan; Beura, Sashikala; Palai, Sidhartha Kumar; Mohanty, Simanta
The experiment was conducted at All India Coordinated Research Project on Floriculture, Biotechnology-cum-Tissue Culture Center, Odisha University of Agriculture and Technology, Bhubaneswar, Odisha during the year 2019 - 2020 entitled “Varietal performance of tuberose (Polianthes tuberosa L.) in Bhubaneswar condition.” Twelve tuberose varieties were studied in Randomized Block Design with three replications. The varieties studied were Arka Nirantara, BR-1, BR-2, BR-3, Calcutta Single, GKTC-4, Hyderabad Single, Mexican Single, Prajwal, Phule Rajani, Pratap Rajani, Shringar. After studying all the parameters it was revealed that Prajwal performed better than the other tuberose varieties in terms of vegetative characters viz. plant height (46.17 cm) and number of leaves per plant (87.33), apart from these variables, Prajwal performed well as compared to others in terms of various flowering characters viz. spike length (89.50 cm), rachis length (43.67 cm), number of florets per spike (55.33), weight of individual floret (1.50 g), weight of florets per spike (83.04 g), number of spikes per clump (4.33), number of spikes per plot (63.33), diameter of cut spike (0.77 cm) and weight of florets per plot (5.28 Kg) , while first floret initiation took the minimum days (12.67 days) from the day of spike emergence. Prajwal was also found to be superior in terms of various bulb characters like, bulb diameter (2.33 cm), bulb length (6.03 cm), and diameter of bulblet (0.77 cm) and bulbs per clump (5.33).
Open Access
Studies on tinting of tuberose and vase life of tinted tuberose (Polianthes tuberosa L.) cv. Calcutta Double
(Department of Floriculture and Landscaping, OUAT, Bhubaneswar, 2021) Panda, Kakali; Palai, Siddharth Kumar; Nath, Manas Ranjan; Mohanty, Simanta
The present investigation entitled “Studies on tinting of tuberose and vase life of tinted tuberose (Polianthes tuberosa L.) cv. Calcutta Double”, was carried out in the laboratory of All India Co-ordinated Research Project on Floriculture, Biotechnology-cum-Tissue Culture Center, Department of Floriculture and Landscaping, College of Agriculture, O.U.A.T., Bhubaneswar, Odisha during the year 2020-21. Experiments were conducted to standardize the optimum concentration of dye, time of harvest of spike and duration of treatment with the dye to get the best tinted spike of tuberose. The tinted tuberose spikes obtained from the best treatment were subjected to vase life study. The experiment was laid out in completely randomized design with 13 treatments and 3 replications. The treatments were T1 -Orange Red, T2- Orange Red + Sucrose (2%) + Citric Acid (300 ppm), T3 - Orange Red + Sucrose (2%) + Aluminium Sulphate (200 ppm), T4 - Orange Red + Sucrose (2%) + HQS (300 ppm), T5- Apple Green, T6- Apple Green + Sucrose (2%) + Citric Acid (300 ppm), T7- Apple Green + Sucrose (2%) + Aluminium Sulphate (200 ppm), T8-Apple Green + Sucrose (2%) + HQS (200 ppm), T9-Lemon Yellow, T10- Lemon Yellow + Sucrose (2%) + Citric Acid (300 ppm),T11- Lemon Yellow + Sucrose (2%) + Aluminium Sulphate (200 ppm), T12- Lemon Yellow + Sucrose (2%) + HQS (200 ppm), T13- Control ( without tinting). The result of the study revealed that there is significant difference among the treatments in parameters such as vase life, change in fresh weight, percent of open flowers and water uptake throughout the vase life. Tuberose spikes in all treatments absorbed colour and looked attractive as only white tuberose is available in nature. The vase life of treated spikes varied significantly between the treatments. Maximum vase life of 11.67 days was observed in T2 - (Orange red+ sucrose(2%)+ citric acid (300 ppm) and T6- (Apple green+ sucrose(2%)+citric acid(300 ppm) and it was at par with T10 - (Lemon yellow + sucrose(2%)+citric acid(300 ppm). The quantity of dye uptake by the spikes and basal floret diameter did not vary significantly between the treatments. Maximum percentage of flowers opened in T2 (Orange red+ sucrose(2%)+ citric acid (300 ppm). The quantity of water uptake (ml/spike) was also maximum in T2 (Orange red+ sucrose(2%)+ citric acid (300 ppm). Hence, the tuberose spikes treated with sucrose (2%) and citric acid (300ppm) irrespective of the food dye exhibited maximum vase life.

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