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ThesisItem Open Access Seed Technological Investigations to Enhance Seed Yield, Quality and Storability in Onion (Allium cepa L.) cv. Arka Kalyan(University of Agricultural Sciences, Dharwad, 2017-07) Lamani, Koteshi; Deshpande, V.K.ThesisItem Open Access Influence of Provenances, Seed Forms, Storage Conditions and Packaging Materials on Seed Longevity in Groundnut(University of Agricultural Science, Dharwad, 2017-08) Sujatha M.; Uppar, D.S.The investigation was undertaken to know the influence of provenances, seed forms, storage conditions and packaging materials on seed longevity in groundnut. The results were analysed in CRD with factorial concept. The seed materials were produced at different locations viz., Dharwad, Bagalkot and Kumta during summer season of 2015-16 and stored during 2016-17 in their respective places under ambient condition and cold storage condition at cold storage unit Dharwad in HDPE bag, polylined gunny bag and vacuum packing in the form of pod and kernel for 12 months. Seed materials were collected bimonthly from respective places to assess the seed quality parameters in laboratory of Department of Seed Science and Technology and National Seed Project, University of Agricultural Sciences, Dharwad. The results revealed that the seeds produced in Bagalkot (L1) and stored as pod in vacuum packing under ambient and cold condition recorded significantly higher seed germination (68.00 and 69.75 %), seedling length (18.36 and 18.20 cm), seedling vigour index (1248 and 1270), seedling dry weight (231 and 245 mg), mobilization efficiency (6.21 and 6.53 %), oil content (47.59 and 47.88 %) and protein content (31.06 and 31.19 %), respectively at the end of 12 months of storage compared to other locations, seed forms and packaging material. Other seed quality parameters like electrical conductivity (0.929 and 0.776 dS/m), lipase activity (0.54 and 0.53 μ moles min-1), protease activity (0.262 and 0.245 U/ml), moisture content (7.05 and 7.31 %) were significantly lower throughout the storage period in the pods produced in Bagalkot and stored in vacuum packing under both ambient and cold condition respectively, compared to other treatments. Bagalkot is suitable for both production and storage of summer groundnut and the seeds stored in vacuum packing showed better storability under both storage conditions.ThesisItem Open Access tudies on Age of Seedlings, Fruit Retention Load, Shoot and Fruit Borer Control and Seed Invigoration Techniques to Enhance Seed Yield and Quality in Brinjal (Solanum melongena L.)(University of Agricultural Science, Dharwad, 2016-06) Bhanuje, Tejagouda; Raikar, S.D.The field experiments were conducted at farmer’s field Chinchali village, Rayabag taluka, Belagavi District, Karnataka during kharif 2014-15 and 2015-16 and laboratory experiment were conducted in Department of Seed Science and Technology, University of Agricultural Sciences, Dharwad during 2014-15. The results revealed that, retaining all fruits for seed purpose recorded significantly higher seed yield (369 kg/ha) as compared to plucking initial formed 18 fruits of green stage for vegetable purpose and retaining remaining fruits for seed purpose. For seed production of brinjal, plucking initial formed 3 fruits of green stage for vegetable purpose and retaining remaining fruits for seed purpose compared to retaining all fruits for seed purpose was found to be most ideal as it registered on par test weight, number of seeds per fruit, seed yield with better quality parameters and an extra income of Rs. 14,395 per hectare was obtained. Among the foliar spray with insecticides, emamectin benzoate was most effective in suppressing the pest population as lowest mean per cent fruit infestation and seed damage was recorded after its application (15.50 and 12.33 % respectively) followed by flubandamide (16.71 and 13.09 %) and spinosad (18.17 and 14.93 %). Brinjal seeds primed with GA3 50 ppm and PEG @ 1.25 MPa and drying them back to their original moisture content needs to be practiced to obtain higher seed quality. The optimum yield with better quality seed can be obtained from the brinjal cv. Kudachi local when 35 days old seedlings were transplanted with GA3 @ 50 ppm growth regulator spray.ThesisItem Open Access Seed Technological Research on Hybrid Pigeon Pea (ICPH-2671)(University of Agricultural Science, Dharwad, 2016-06) Chandravathi B.; Gurumurthy R.The field and laboratory experiments were conducted to study the effect of pollination methods, planting ratio’s, insect attractants and morphological and molecular characterization of parental lines of hybrid pigeon pea ICPH-2671 at Seed Science and Technology, Department, College of Agriculture, University of Agricultural Sciences, Dharwad during kharif-2011 and 2015. Among pollination methods, P3: plant to progeny row selection recorded significantly the highest number of seeds per pod (4.23), hundred seed weight (12.01 g) and seedling vigour index (2963) as compared to P1: selfing and P2: random mating pollination methods. The treatment T2: insect attractant spray Bee-Q at the rate of 12.5 g/liter of water recorded significantly the highest number of seeds per pod (3.56 seeds /pod), seed yield per plant (62.68 g/plant), seed yield per plot (4.90 kg/plot) and seed yield per ha (2243.00 kg/ha), germination (93.39 %), shoot length (15.75 cm), root length (12.32 cm), seedling vigour Index (2627), seedling dry weight (0.91 g), hundred seed weight (12.19 g) and low electrical conductivity (0.39 dS/m) as compared to T1: control (Without insect attractant spray) in F1 hybrid ICPH-2671 seed production. Among the different planting ratio’s the P6: 5:2 and P5: 4:2 female to male planting ratio’s recorded significantly higher seed yield and quality as compared to P1: 3:1, P2 :4:1, P3:5:1 and P4:3:2 planting ratio’s in F1 hybrid ‘ICPH-2671’seed production. The study of morphological characterization of parental lines of the hybrid pigeon pea ‘ICPH-2671’ is useful for identification of off types in hybrid pigeon pea seed production mainly to carry out the rouging operation. In molecular markers characterization study the SSR primers CcM-0021 and CcM-1707 showed DNA amplification pattern between F1 hybrid and its parental lines of hybrid pigeon pea ‘ICPH-2671’ these primers can be employed for detection of genetic purity.ThesisItem Open Access Seed Technological Approaches to Enhance Seed Yield and Quality In Perennial Fodder Sorghum (Sorghum bicolor L.)(University of Agricultural Science, Dharwad, 2015-06) Patil, Bapurayagouda; Merwade, M.N.The field experiments were conducted at Main Agricultural Research Station, Dharwad during kharif 2012-13 and 2013-14 and laboratory experiment were conducted in Department of Seed Science and Technology, University of Agricultural Sciences, Dharwad during 2013-14. The results revealed that, the inter row spacing of 30 cm recorded significantly higher seed yield (657.6 kg ha-1) as compared to 45 and 60 cm row spacings. In contrary, inter row spacing of 60 cm recorded significantly higher seed quality parameters as against to other row spacings. Application of 150:60:40 NPK kg ha-1 significantly higher seed yield (641.6 kg ha-1) and seed quality parameters. Whereas, the treatment combination of 30 cm row spacing and 150:60:40 NPK kg ha-1 recorded higher seed yield. The early sowing of crop in July IV week recorded significantly higher seed yield (593.4 kg ha-1), seed germination (39.67%) and seedling vigour index (1065) as compared to later sowings. The intra spacing of 45 x 10 cm recorded higher seed yield (492.4 kg ha-1) as against 45 x 15 and 45 x 20 cm spacings. The combination of July IV week sowing and 45 x 10 cm intra row spacing recorded higher seed yield (598.8 kg ha-1) as compared to other treatment combinations. Among the number of cuttings, uncut crop (control) recorded significantly maximum seed yield (576.4 kg ha-1), seed germination (40.00%) and seedling vigour index (1126) as compared to one, two, three and four cuttings. Seeds treated with KNO3 @1.0 % for 2 hrs found effective in breaking seed dormncy of perennial fodder sorghum cv. CoFS-29. It was recorded significantly higher seed germination (82.33%), seedling vigour index (2303), lesser dormant seeds (5.67 %) and dead seeds (7.00%) at the end of ten months of storage period which was followed by Thiourea @1.0 % for 2 hrs.ThesisItem Open Access Studies on Seed Production Techniques, Storability and Morphological Characterization of Fodder Maize (Zea Mays L.)(University of Agricultural Science, Dharwad, 2015-06) Patil, Shivagouda; Merwade, M.N.Three field experiments were conducted in fodder maize during 2013-14 and 2014-15 at MARS, UAS, Dharwad on effect of spacings, fertility levels, micronutrient application methods on growth, seed yield and quality traits and morphological characterization of thirteen genotypes and laboratory experiment was also conducted on effect of chemical and botanical seed treatments on seed storability under ambient conditions for twelve months. In first experiment, the combined effect of 60 x 30 cm row spacing and 150:100:50 kg N, P2O5, K2O ha-1 recorded significantly maximum seed yield (31.58q/ha) as against 75 x 30 cm and 75:100:50 kg N, P2O5, K2O ha-1 + 50% N supplemented through FYM (27.89 q/ha). Whereas, seed germination (98.50%), seedling vigour index (4164) were highest in 75 x 30 cm and 150:100:50 kg N, P2O5, K2O ha-1 as against 60 x 30 cm and 75:100:50 kg N, P2O5, K2O ha-1 + 50% N supplemented through FYM (93.50% and 3559, respectively). Soil application of Zinc sulphate @ 25 kg ha-1 + foliar spraying of 0.2% boron at 50 % flowering along with 150:100:50 kg N, P2O5, K2O ha-1 (RDF) recorded significantly highest seed yield (122.38g) per plant and (32.57 q.) hectare, seed germination (99.17%) due to better growth, flowering and yield components. Seeds treated with vitavax power @ 3 gkg-1 of seeds retained higher 100 seed weight (29.70g), germination (90.50%), seedling vigour index (2891) and least seed infection (5.55%) as against untreated control seeds throughout twelve months ambient storage period. Thirteen fodder maize genotypes were grouped based on DUS guidelines as presence and absence of brace root anthocynin colouration, straight and drooping leaf attitude, silk colour, dent and flint grain types, white and orange seed colour. Among different characters studied, anthocynin colouration of brace roots was distinctly absent in the MFM-07-03, MFM-07-07 and MFM-07-11 genotypes over other genotypes.ThesisItem Open Access STANDARDIZATION OF SEED PRODUCTION TECHNOLOGY AND MOLECULAR CHARACTERIZATION OF HYBRID SUNFLOWER (DSFH-2)(University of Agricultural Sciences, Dharwad, 2015-06) SHANKRAYYA; VYAKARANAHAL, B. S.The field experiments were conducted to study the effect of staggered sowing on flowering synchrony, seed yield and quality of sunflower hybrid DSFH-2 consisted of seven staggered sowing treatments with three replications. Significantly higher head diameter (13.15 cm), number of seeds per head (946.17), head dry weight (86.78 g), number of filled seeds per head (783.85) and its weight (35.07 g), seed set (82.76 %), seed weight per plant (38.50 g) and seed yield per hectare (12.38 q/ha) was significantly higher when the male parent was sown two days later to the female parent followed by four later sowing. Effect dates of sowing on flowering behaviour of parental lines of sunflower hybrid DSFH-2 nine different sowing dates with three replications. The results revealed that male parent (RHA-16) has to be sown one to two days later to female parent (CMS-234A) between July to September and January to March sowing whereas, two to three days later to female parent in October to December sowing. Effect of foliar application of growth regulators and micronutrients on flowering synchrony, seed yield and quality of sunflower hybrid DSFH-2 consisted of foliar spray of ten growth regulators and micronutrient treatments with three replications. Significantly higher head diameter (13.03 cm), number of seeds per head (938.50), head dry weight (88.25 g), number of filled seeds per head (794.15) and its weight (34.20 g), seed set (84.19 %), seed weight per plant (40.77 g), seed yield per hectare and also higher seed quality parameters like germination (95.00 %), seedling vigour index (3072), shoot length (17.43 cm), root length (14.90 cm), seedling length (32.33 cm), dry weight of seedling (0.44 g), volume weight of seed (46.82 g) 100 seed weight (4.72 g), oil content (37.47 %), field emergence (89.50 %) and lower values of electrical conductivity (0.355 dSm-1) were significantly highest (11.73 q/ha) due to foliar application of GA3 @ 50 ppm (T6) which was followed by boron @ 0.2 per cent (T5) (11.49 q/ha) and ZnSO4 @ 0.5 per cent (T9) (11.24 q/ha) respectively. Sunflower hybrids could be distinguishable clearly from their parental lines using SSR markers. HA3640 and HA4011 can be confidently used for identification of DSFH-2, but the SSR marker ORS 691 can be used for identification as well as for seed genetic purity test of DSFH -3.ThesisItem Open Access STUDIES ON SEED YIELD, QUALITY AND SEED LONGEVITY IN SOYBEAN (Glycine max (L.) Merrill.)(University of Agricultural Sciences, Dharwad, 2014-11) BHARAMARAJ BADIGER; Dr. RAVI HUNJEThe field experiments were conducted at Water and Land Management Institute (WALMI), Dharwad while, storage and molecular studies were conducted at Seed Quality and Research Laboratory, National Seed Project, University of Agricultural Sciences, Dharwad during 2012-13 and 2013-14. Foliar application of chemicals exhibited significant variation on seed yield and quality components of different soybean genotypes. FeSO4 @ 0.50% foliar application recorded higher seed yield (22.49 q/ha), seed germination (92.0%) and seedling vigour index-I (2878) compared to control. Among the genotypes, JS-335 exhibited significantly higher seed yield (23.45 q/ha) compared to other genotypes. Seed lots revealed significant variation on seed yield, quality and storability during pulsed electromagnetic field treatments on soybean cv. JS-93-05. Fresh seed lot recorded higher seed yield (21.70 q/ha), seed germination (91.5%) and seedling vigour index-I (3035) compared to revalidated seed lot Whereas, pulsed electromagnetic field treatment 50 Hz (F4) recorded significantly higher seed yield (22.63 q/ha), seed germination (92.5%) and seedling vigour index- I (3127) compared to control . At the end of 12 month storage period 50 Hz (F4) pulsed electromagnetic field treatment recorded the higher seed germination (49.2%) and seedling vigour index-I (716) in contrast to control Among the varieties stored, Kalitur (71.3%) and Birsa soya (70.0%) recorded germination percentage above minimum seed certification standard (70%) up to ten months of storage period compared to other varieties. Molecular screening with SSR marker revealed that, primer Satt 316 gave distinct banding pattern between good and poor longevity genotypes. From the present investigation, Kalitur, Birsa soya and JS-9305 genotypes were recognized as better storer.ThesisItem Open Access STANDARDIZATION OF SEED PRODUCTION TECHNOLOGIES IN LUCERNE (Medicago sativa L.)(University of Agricultural Sciences, Dharwad, 2014-05) ARUNAKUMAR; Dr. V. K. DeshpandeAn investigation entitled “Standardization of seed production technologies in lucerne (Medicago sativa L.) Cv. RL-88” was carried out during rabi-summer seasons of 2010-11 and 2011-12 at Indian Grassland and Fodder Research Institute, Southern Regional Research Station, Dharwad and Department of Seed Science and Technology, University of Agricultural Sciences, Dharwad with four field and two laboratory experiments. The results on the cutting management practices revealed that two cut (T3) treatment recorded significantly the highest seed yield (228.93 kg/ha.), seed quality parameters like seed germination (87.50 %), seedling vigour index (1152). Among the different time of sowing treatments, sowing on December 14th registered significantly higher growth, seed yield (222.41 kg/ha.), seed quality like seed germination (87.13 %) and seedling vigour index (1137). Seeds harvested from two cut treatment and 14th December sown crop showed higher seed quality parameters during all the months of ambient storage over other cutting management and time of sowing treatments. The seeds stored in polyethylene bag (700 gauge) were superior over cloth bag throughout the storage period. Among the supplementary pollination techniques, spraying of Jaggery solution (10 %) recorded highest seed setting (61.73 %), seed yield (297.90 kg/ha) and seed quality traits like seed germination (87.59 %), seedling length (12.89 cm) and seedling vigour index (1129) as compared to other techniques. The experiment on seed development and maturation studies revealed that harvesting of pods and seeds on 35 days after anthesis (DAA) produced higher seed quality attributes like seed germination (85.67 %), seedling length (13.86 cm), seedling vigour index (1187). It also indicated that harvesting pods on 35th DAA is the actual physiological maturity stage for obtaining better seed quality in lucerne.