Thumbnail Image

Theses

Browse

20095
Reset filters
End date: 2009 × Start date: 2009 × Thesis Type: M.V.Sc. ×
Reset filters

Search Results

Now showing 1 - 5 of 5
  • Thumbnail Image
    ThesisItemOpen Access
    Immunopathological effects of kitazin, an organophosphorous pesticide in broilers
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2009-05) Kohila, P.; Thathoo, A.K.
    The present experiment was carried out to assess the acute and sub acute toxic effects of Kitazin, an organophosphorous pesticide in broiler chicken. To assess the acute toxic effects of Kitazin, a total of 48 day old broiler chicks were randomly divided in four groups of 12 birds each. After one week of acclimatization period, a single oral dose of Kitazin was administered @ 300mg, 600mg and 900 mg/ Kg body weight to group 2 (T1), group 3 (T2) and group 4 (T3) respectively. To assess the sub acute toxic effects of Kitazin, a total of 48 day old broiler chicks were randomly divided in four groups of 12 birds each. After one week of acclimatization period, Kitazin was incorporated in the feed @ 100 ppm, 200 ppm and 400 ppm to group 2 (T1), group 3 (T2) and group 4 (T3) respectively for a period of 6 weeks. In both the studies the group 1 birds served as control (C). Blood and serum samples were collected once in a week in acute toxicity study, and once in two weeks interval in sub acute toxicity study to assess the haematological and biochemical parameters respectively. In acute study, the birds were sacrificed at the end of 14th and 21st day of age, to study the pathomorphology of organs. In subacute study, the birds were sacrificed on 49th day of age. Acute toxicity of Kitazin produced typical neurological signs such as ataxia, in coordination, drooping of wings, tremors, convulsions and death. The average body weights of experimental birds measured at once in 14 days interval revealed a significant decrease in Kitazin treated birds in both acute and sub acute toxicity study. Effects of Kitazin on the haematological parameters were significantly decreased haemoglobin, PCV and TEC values in both acute and sub acute toxicity study. A significant decrease in TLC, lymphocyte count, MCV and MCH values were also observed. The results of the biochemical studies had a significant increase in serum glucose, total cholesterol, SGOT and creatinine values in both the studies. A significant increase in SGPT and BUN values were observed in sub acute toxicity study. The levels of total proteins, albumin, globulin and A/G ratio were significantly decreased in both the studies. Results of Lymphocyte Stimulation Test (LST) showed a significant decrease in mean delta OD values of both Con-A and LPS stimulated lymphocyte cultures from treatment groups in comparison to control group.A significant reduction in the organ/body weight ratio was observed for liver, spleen and bursa in both the studies. Grossly, congestion of all organs was noticed. Histopathological examination of liver showed hyperemia, mononuclear cell inifiltration and hepatocytes undergoing various stages of degeneration. Kidney showed glomerular nephritis, nephrosis and haemorrhages. Desquamation of bronchial epithelium, edema, emphysema and congestion were observed in the lungs. Degenerative and necrotic changes were observed in the spleen and bursa. The lesions in the brain were congestion, perivascular cuffing, chromatolysis, neuronophagia and extensive spongiform changes. From the findings of the present study, it may be concluded that, Kitazin has adverse effects on the general health, body weight and immune status of the birds and these effects are dependent on its dose and duration of exposure.
  • Thumbnail Image
    ThesisItemOpen Access
    A study on some characteristics of Lochia collected from crossbred cows
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2009-07) Suresh Kumar, N.; Singh, D.V.
    The study was conducted from October, 2008 to March, 2009 at Pantnagar on lochia (post-partum uterine secretion) collected from ten crossbred cows. The objective was to study physical, biochemical and microbial nature of lochia. On an average 21.3±1.7 ml of lochia could be collected from the cows. The colour of lochia was reddish brown, due to the autolysis and liquefaction of most superficial layers of uterine epithelium. The consistency of lochia was medium and slightly thicker than water and blood. The mean pH and electrical conductivity of lochia were 6.63±0.06 and 3.79 ± 0.64 ms/m, respectively. Typical fern pattern of cervical mucus of estrus was not observed with lochia. The mean glucose, urea nitrogen, albumin, globulin, total protein, calcium, phosphorus, sodium, potassium and chloride concentrations of lochia samples were19.97±1.77 mg/dl, 30.99±3.33 mg/dl, 2.30 ± 0.09 g/dl, 4.69±0.14 g/dl, 7.00±0.20 g/dl, 11.26±0.46 mg/dl, 0.38±0.02 mg/dl, 116.50±4.96 mEq/l, 5.46±0.34 mEq/l, and 98.86±3.66 mEq/l, respectively. The glucose concentration in lochia samples was higher than that of uterine luminal fluid but lower than that of blood serum. Urea nitrogen content of lochia was higher than that of normal blood serum level. Protein concentrations in lochia samples were within normal range of blood serum. The calcium content in lochia was more than that of post-partum uterine fluid and blood serum level. Potassium and serum were within normal range of blood serum. The mean bacterial load of lochia was (4.53±0.48) x 103/ml, and significantly lower than lochia collected during uterine infections. Above findings indicate that lochia may be considered as uterine secretory product. Further analysis of lochia may reveal some specific constituents in it which may have role in improving fertility.
  • Thumbnail Image
    ThesisItemOpen Access
    Studies on morphological and performance traits of feathered shank local hill fowl
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2009-06) Sharma, Naresh Chand; Singh, S.K.
    The present study was conducted to evaluate the morphological and performance traits of feathered shank local hill fowl.” The average body weight at day old, 1, 2, 3, 4, 5, 6, 8, 10, 12, 14, 16, 18 and 20 weeks of age were 30.84±0.18, 43.81±0.23, 51.83±0.29, 84.27±0.60,107.11±0.52, 185.60±0.70, 251.03±0.82, 373.56±1.62, 513.99±1.71, 585.01±2.21, 737.49±3.83, 879.35±4.81, 965.90±4.94, and 1194.17± 6.25 g. Feathered shank local hill fowl males had prominently mixed colours in their plumage with, black brown gold (43%), followed by black brown, black white, black golden and black red brown white. However, female birds had 50% single colour plumage; most frequent single colour was founded to be black followed by brown, dusty brown and almond colour. Feathered shank females also had notably mixed colour plumage (50%). It was found that feathered shank local hill fowl dominated with single type comb i.e. 99% and 98% in males and females birds, respectively. However, (2%) female birds possess pea type comb and male birds occasionally have rose comb (1%). Ear lobe was found to be mainly white red (46%) followed by yellow red (30%) and red (24%) in colour in feathered shank male hill fowl. However, red colour predominantly distributed (46%) in females followed by white red (44%) and yellow red (10%). Eyes were predominantly brown-black (45% in males and 54% in females), followed by grey-black (44% in males and 34% in females), orange-black (8% in males and 5% in females), total black (3% in males and 4% in females); and grey brown (3% in females only) in feathered shank local hill. The skin colour in feathered shank hill fowl was generally yellow. It was noted that black yellow colour shanks were the more frequently (53% in males and 61% in females) encountered in the feathered shank local hill fowl, followed by yellow shanks (28% in males and 5% females) and white yellow colour shanks (19% in males and 34% in females). The averages of heamoglobin packed cell volume (PCV), total leucocytes count (thousands /cmm), lymphocytes count, heterophils, monocytes, basophils, eosinophils and H: L ratio were found to be 12.94±0.06 g/dl, 39.6±0.25%, 24.04±0.10X103/cmm, 68.98±0.88, 16.40±0.66, 5.03±0.34, 2.46±0.11%, and 0.24±0.01 male birds and 9.73± 0.06 g/%, 31.6± 0.65%, 23.96±0.08 x103/cmm, 68.06±0.71, 10.11±0.83, 14.05±0.61, 4.43±0.14, 2.29±0.16% and 0.21±0.61 in female birds. The averages of total protein, albumin, and globulin, cholesterol, glucose and A: G ratio were 4.62±0.03 g/dl, 1.85±0.01 g/dl, 2.77±0.03 g/dl, 114.51±2.00 mg/dl, 235.62±0.92 mg/dl and 0.67±0.01 in male and 4.14±0.02 g/dl, 1.75±0.00 g/dl, 2.39±0.03 g/dl, 113.421±0.68 mg/dl, 234.38 ±1.64 mg/dl, 0.74 ±0.01 in female, respectively. Fertility, hatchability on the basis of fertile egg set and total egg set were 71.16±1.16, 85.32±1.68 and 60.78±1.70 per cent in feathered local hill fowl, respectively. The average live weight at twenty weeks of age, dressed weight, eviscerated weight, giblet weight, skin weight, feet and shank weight, head weight, feather weight, wing weight and neck weight were 1202.00±37.98, 922.80±31.37, 750.40±28.03, 66.60±1.13, 161.00±1.63,66.4±2.41, 42.20±1.55, 84.00±3.87, 90.60±3.29 and 54.40±2.12 g in male and 1044.00±6.68, 812.00±5.01, 643.00±3.43, 61.80±0.53, 151.00±2.41, 49.8±1.06, 37.80±0.58, 81.00±2.21, 83.2±1.24,and 48.20±1.69g in female, respectively .the average egg weight, shape index, haught units, shell thickness, specific gravity, albumin index, yolk index, albumin weight, albumin volume, yolk weight and shell weight in feathered shank local hill fowl were 52.74±0.62g, 70.61±1.05, 76.22±2.82, 0.38±0.01mm, 1.08±0.03, 0.09±0.01, 0.50±0.01, 27.75±0.74g, 25.70±0.25ml, 16.93±0.37g and 5.02±0.16 g, respectively.
  • Thumbnail Image
    ThesisItemOpen Access
    Studies on morphological and performance traits of clean shank local hill fowl
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2009-06) Singh, Abhay Kumar; Singh, D.V.
    Present study was conducted to evaluate the morphological and performance traits in clean shank local hill fowl of Nainital region. Clean shank local hill fowls had mixed colour plumage. Most prominent was white colour, followed by black-white, black, golden with black spot, black-brown, brown-gold, black dark green spot on feathers and white with brown. Skin colour was generally yellow, followed by white, and black. Shank colour was mostly yellow, followed by black, white yellow, and white. Earlobe was found to be mainly red and white red. Eyes were predominantly greyblack, followed by black-brown, black and grey in clean shank local hill fowl. Majority of comb colour was found red with a few having pale comb and black colour comb. Mean body weight at day old, 1, 2, 3, 4, 5, 6, 8, 10, 12, 14, 16, 18 and 20 weeks of age were 31.84±0.24, 47.07±0.40, 57.84±0.38, 81.23±0.74, 114.47±0.53, 176.52±0.70, 267.3±1.02, 380.95±1.12, 526.65±0.93, 628.05±0.91, 762.25±1.17, 923.9±6.0, 1025.45±1.17 and 1206.55±6.26 g, respectively. Mean values of haemoglobin, PCV, TLC, lymphocytes, monocytes, heterophils, eosinophils and H:L ratio at 20 weeks of age were found to be 12.78±0.32 g%, 40.4±0.52%, 2.33±0.032 103/mm3, 67.4±0.94%, 7.5±0.40%, 20.1±0.86%, 4.2±0.35% and 0.29±0.01, respectively in male birds and 10.24±0.48 g%, 38.7±0.36%, 2.33±0.02 103/mm3, 64.4±0.88%, 7.4±0.42%, 23.4±0.83%, 4.7±0.49% and 0.35±0.01, respectively in female birds. Averages of total protein, albumin and globulin, cholesterol, glucose and A:G ratio at 20 weeks of age were 4.12±0.11 g/dl, 1.83±0.08 g/dl, 2.28±0.12 g/dl, 115.05±0.73 mg/dl, 234.04±0.88 mg/dl, 0.82±0.07 in male and 3.85±0.03 g/dl, 1.73±0.08 g/dl, 2.11±0.09 g/dl, 115.24±0.72 mg/dl, 233.25±0.85 mg/dl, 0.84±0.09 in female birds, respectively. Age at first egg laid, fertility, hatchability on the basis of fertile egg set and total egg set were 186.2±1.21 days, 82.0±1.0%, 88.4±0.82% and 74.4±0.82%, respectively in clean shank local hill fowl. Mean live weight, dressed weight, eviscerated weight, weight of giblet, skin, feet and shank, head, feather, wing and neck at 20 weeks of age were 1248±13.52, 974.0±14.42, 788.6±6.55, 61.40±0.65, 165.2±1.43, 70.6±0.96, 46.6±0.42, 87.2±0.81, 94.4±0.82 and 60.4±0.52 g in male and 1277±16.56, 945±21.79, 782±9.65, 60±1.20, 164.8±0.95, 56.2±1.23, 45.6±0.36, 84.0±1.0, 93.4±30.98 and 59.4±0.76 g in female birds, respectively. Average egg weight, shape index, haugh unit, shell thickness, specific gravity, albumin index, yolk index, albumin weight, albumin volume, yolk weight and shell weight in clean shank local hill fowl were 51.41±0.56 g, 72.86±0.12, 78.56±0.45, 0367±0.016 mm, 1.08±0.004, 0.083±0.004, 0.498±0.008, 30.40±0.46 g, 29.5±0.34 ml, 16.80±0.19 g and 6.25±0.18 g, respectively.
  • Thumbnail Image
    ThesisItemOpen Access
    Effect of addition of lochia on some characteristics of crossbred bulls’ semen
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2009-06) Iqbal, Syed Mohsin; Singh, D.V.
    The study was conducted on lochia (postpartum uterine secretion) samples collected from ten crossbred cows and semen from two crossbred bulls. The objective was to standardize the technique of lochia collection from crossbred cows and to study the effect of addition of lochia on progressive sperm motility percentage, live sperm percentage and abnormal sperm percentage of crossbred bulls’ semen. Lochia collection from uterus was done by using Foley’s catheter with syringe which helped to collect varying amount of lochia aseptically. The lochia added semen samples were divided into three treatment groups (T1, T2 and T3) composing different (0.5 ml, 1 ml and 2ml, respectively) volumes of semen and 0.1 ml of lochia. In Control group 0.1 ml normal saline was added instead of lochia in semen sample. The values of overall mean of progressive sperm motility1, Live2 and abnormal3 sperm count in T1, T2, T3 and control group were (64.9 ± 1.7, 36.0 ± 0.6 and 13.6 ± 0.9)1, (64.6 ± 2.0, 66.1 ± 1.8, 65.0 ±2.2, 66.3 ±1.8)2 and (4.9 ± 0.7, 4.8 ± 0.8, 3.9 ± 0.7 and 4.4 ± 0.6)3 at 0 hour, (36.0 ± 0.63, 36.6 ± 1.18, 35.2 ± 1.25, and 37.1 ± 1.32)1, (43.2 ± 2.0, 42.1 ± 1.7, 43.1 ± 2.2 and 42.7 ± 1.8 )2 and (5.5 ± 0.6, 5.3 ± 0.6, 4.9 ± 0.9 and 4.4 ± 1.0)3 after 4 hours and (13.6 ± 0.97, 12.7 ± 1.20, 14.3 ± 1.19 and 14.57 ± 1.35)1, (25.3 ± 1.6, 24.1 ± 1.1, 26.0 ± 1.3 and 24.2 ± 1.3)2 and (5.5 ± 0.6, 5.3 ± 0.6, 4.9 ± 0.9 and 4.4 ± 1.0)3 after 8 hours, respectively. There were no significant differences among these values of control and treatments. The results show that the lochia is not detrimental to the survivability of the sperm. Further detailed investigation with more purified lochia at other dilution rates is needed.