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Author: Mandeep Kaur × End date: 2016 × Start date: 2000 × Type: Thesis ×
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    ThesisItemOpen Access
    DEVELOPMENT OF ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA) FOR DETECTION OF MELOIDOGYNE SPP. (ROOT-KNOT NEMATODE) ANTIGENS
    (PAU, 2010) Mandeep Kaur; Ghai, Jyotika Kapur
    Plant parasitic nematodes are destructive pests worldwide that cause severe losses in agriculture. Root-knot nematodes (Meloidogyne spp.) are obligate, sedentary plant endoparasites that are extremely polyphagous in nature with a wide host range. Keeping in view the huge economic losses by this parasite, it is essential to control the disease at an early stage. For any control strategy to be effective, an early and accurate diagnosis is of paramount importance. Immunoassays have the inherent advantages of sensitivity and specificity; have the potential to identify and quantify these plant-parasitic nematodes. Hence, in the present studies, ELISA has been developed for the detection of Meloidogyne spp. antigens. This is based on detection of egg antigens, for which anti-Meloidogyne antibodies were produced by immunization of rabbits with soluble proteins of the eggs. The production of antibodies was confirmed by the appearance of precipitin lines in double immunodiffusion method. An indirect enzyme-linked immunosorbent assay (ELISA) was developed for detection and titration of these antibodies. Results indicated as high as 320 K titre of the antisera. Finally competitive inhibition ELISA was developed employing these anti-Meloidogyne antibodies for detection of Meloidogyne antigens. This is based on competition between solid phase bound antigens and free antigens for limited antibodies. Sensitivity of ELISA was 10 femtograms. This sensitivity was further enhanced to 1 femtogram with an additional step of pre-incubation, in which antibodies and free antigens are allowed to react before the competition. Competitive inhibition ELISA developed in the present studies has the potential of being used as an easy, rapid, specific and sensitive diagnostic tool for the detection of Meloidogyne infection.
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    ThesisItemOpen Access
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    ThesisItemOpen Access
    Impact Of Dietary Counselling On Nutritional Status Of Senior Citizen In Punjab
    (Punjab Agricultural University ;Ludhiana, 2000) Mandeep Kaur; Murgai, V.
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    ThesisItemOpen Access
    Health Problems In Rural Punjab : Status, Causes And Consequences
    (Punjab Agricultural University; Ludhiana, 2005) Mandeep Kaur; Sukhdev Singh
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    ThesisItemRestricted
    REFASHIONING THE TRADITIONAL CRAFT OF PHULKARI THROUGH COMPUTER AIDED DESIGNING
    (Punjab Agricultural University, Ludhiana, 2014) Mandeep Kaur
    The present study entitled “Refashioning the traditional craft of Phulkari through Computer Aided Designing” was carried out in Ludhiana city. An interview schedule was prepared to document the motifs, fabrics, stitches and colours used in Phulkari products in the last five years (2009-2013) from forty consumers and twenty retailers. The results of the investigation revealed that most of the consumers possessing Phulkari products were females between 35-45 years of age and majority of the retailers offering Phulkari products were males between 35-45 years of age. The documentation revealed that majority of the products available with consumers and retailers were ornamented in the style of Phulkari instead of Bagh. The traditional flower motifs were used in the majority of the consumer and retailer articles. The other popular motifs used were of geometric shapes and leaves. The work was embroidered on chinon chiffon fabric in most of the consumer products whereas, on cambric fabric in most of the retailer products. Majority of the products documented from both consumers and retailers were embroidered with the darn stitch. The red colour was the most popular colour for both base fabric as well as the pat thread in the both consumer and retailer products. The Phulkari designs were then developed and simulated using Corel Draw X3, Adobe Photoshop and Corel Photo-Paint X3 softwares. Later ten fashion articles were prepared using the most preferred embroidery designs of Phulkari for belt, border, jacket, scarf, yoke, footwear, headband, bracelet, handbag and potli bag. On the basis of suitability of the base fabric used, footwear made using velvet and an embroidered strip of voile was preferred the most. The design of the potli bag superseded the designs of other fashion articles as per the consumers taste. According to the colour combination, green base with yellow and magenta coloured embroidery on the footwear was favored by most of the respondents. The footwear was outweighed by the consumers on the basis of the overall impact amongst the fashion articles. Majority of the respondents reported the quoted price for the fashion articles to be suitable. The results intended that the Phulkari fashion articles developed after visualisation of computerized simulated Phulkari patterns are commercially viable and thirty to forty percent profit can be incurred by making innovative fashion articles using Phulkari.
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    ThesisItemOpen Access
    Irradiation induced mutagenesis in sugarcane
    (2014) Mandeep Kaur; Thind, K.S.
    A study on irradiation induced mutagenesis in sugarcane was undertaken to investigate genetic variability for economic traits and red rot disease. Twelve hundred single bud setts of cv. Co J 64 were irradiated with gamma rays at 0, 10, 20, 25 and 30 Grays (Gy). The experimental material of M1V1 generation was planted during spring 2012 and second generation (M1V2) during 2013. Data was recorded on ten economic and quality traits in addition to reaction to red rot disease. Mutagenic treatments generated significant variation for germination, number of tillers/clump, number of millable canes/clump, cane height, HR brix, leaf length and single cane weight in both M1V1 and M1V2 generations indicating the potential of mutagenic treatments for creating genetic variability for different traits in sugarcane. Average cane height among different treatments ranged from 166.60 to 185.80 cm with a mean of 171.2 cm. Mean leaf length for different treatments varied from 106.30 to 114.80 cm with an average of 111.40 cm; maximum of 114.80 cm being recorded through 10 Gy treatment followed by control. Cane weight an important trait in sugar cane breeding programme ranged from 720.00 to 961.66 g. Maximum single cane weight was recorded for control followed by 10 Gy and 20 Gy treatments. The higher doses had a retarding effect on this trait. Artificial evaluation of irradiated subclones against two red rot pathotypes Cf 08 and Cf 09 using plug method revealed moderate resistance for all the mutagenic treatments. It is inferred that genetic variability could be induced in sugarcane using gamma rays for traits like germination, cane height, leaf length, HR brix, single cane weight and to red rot tolerance. The lower doses of gamma ray (10 and 20 Gy) were more effective to induce variation while higher doses have detrimental effects. The sub clones need to be further evaluated in M1V3 for their consistency in trait manifestation and future use in breeding programme.
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    ThesisItemOpen Access
    Effect of planting pattern and straw management on herbicide persistence, productivity and quality of wheat (Triticum aestivum L.) and onion (Allium cepa L.)
    (unjab Agricultural University, 2009) Mandeep Kaur; U.S. Walia
    inconsistent encouragement over the years. The constant affection, eternal blessings and good wishes of elder sister Monia and brothers Balpreet Singh, Harpreet Saini and Randeep Singh and Bhabhi Gurtinder Kaur & Rajwinder Kaur could never be forgotten and would be retained in deeper depths of my interiors. My soulful gratitude to my betterhalf Dr. Jaspreet Singh Saini for his indefinite love, good wishes, support and insist of completion of my studies. This work would not have been accomplished without the constant encouragement, warm blessings and intimate help bestowed upon me by Dr. Navneet Kaur and Mandeep Singh and all the members of my loving in-laws. The constructive moral support and affection received from my divine father-in-law S. Amarjit Singh Saini and mother-in-law Smt. Prabhjot Kaur Saini needs a special mention in the completion of the present studies. How can I forget Tejveer, Yuvneil & Mehtaj whose innocent faces and cheerful cries always enlightened my momentum.
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    ThesisItemRestricted
    Effect of planting pattern and straw management on herbicide persistence, productivity and quality of wheat (Triticum aestivum L.) and onion (Allium cepa L.)
    (PUNJAB AGRICULTURAL UNIVERSITY, 2009) Mandeep Kaur
    The field experiment on “Effect of planting pattern and straw management on herbicide persistence, productivity and quality of wheat (Triticum aestivum L.) and onion (Allium cepa L.)” was conducted at the experimental field of PAU, Ludhiana during rabi seasons of 2006-07 and 2007-08. The studies reveal that growth and development of P. minor were slightly reduced under Happy Seeder sown crop and zero till sowing in standing stubbles than bed sowing, zero tillage after burning and conventional tillage after partial burning. Growth parameters, yield attributes and grain yield of wheat were not significantly influenced by different planting patterns. Conventional tillage and bed sowing treatments slightly improved the physical properties of soil. Application of sulfosulfuron 25 g ha-1 , mesosulfuron + iodosulfuron 12 g ha-1 and pinoxaden 50 g ha-1 significantly reduced the growth and development of P. minor and hence significantly increased grain yield of wheat than unweeded (control). Hectolitre weight, sedimentation value and protein content of wheat grain under different planting patterns were non-significant. Hectolitre weight and sedimentation value were significantly higher in herbicidal treatments than control. Residues of sulfosulfuron 25 g ha-1 , mesosulfuron + iodosulfuron 12 g ha-1 and pinoxaden 50 g ha-1 were detected at 1 day after spray in soil at depth 0-15 cm but residues of herbicides were not detected in soil at other observational periods and in grain and straw at harvest. The highest microbial population was observed under zero till sowing with Happy Seeder followed by zero till sowing in standing stubbles among planting patterns and in unsprayed plots among weed control treatments at all observational period. There was decrease in viable microbial counts at 15 days after spray and further the microbial population started to regain. The bulb yield of onion crop was not significantly influenced with planting patterns and rice straw incorporation levels. However, onion transplanted after rice straw incorporation yielded higher in flat sowing followed by bed sowing techniques. During both the years, application of oxyfluorfen 0.225 kg ha-1 recorded the maximum bulb diameter (60, 90 DAT and at uprooting time) which was statistically at par with pendimethalin 0.75 kg ha-1 and two hand weedings and these treatments were found to be significantly better than fluchloralin 1.125 kg ha-1 and unweeded (control). Application of oxyfluorfen 0.225 kg ha-1 gave the highest bulb yield which was statistically at par with pendimethalin 0.75 kg ha-1 and two hand weedings. These treatments proved significantly better than fluchloralin 1.125 kg ha-1 and unweeded (control) treatment. Also, bulb yield produced by fluchloralin 1.125 kg ha-1 was significantly better than unweeded (control). Soil samples of oxyfluorfen 0.225 kg ha-1, pendimethalin 0.75 kg ha-1 and fluchloralin 1.125 kg ha-1 treated plots taken 1 day after spray showed residues of respective herbicides. Samples of onion bulbs at 30, 60, 90 days after spray and at uprooting stage showed no residues of applied herbicides. The highest microbial population was observed under flat and bed sowing after rice straw incorporation treatments and unsprayed plots among weed control treatments at all observational period. There was decrease in viable microbial counts at 15 days after spray as compared to that at 0 day after spray and further the microbial poatiopuln started to regain.