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2006 - 200912010 - 20183
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Author: Bhupinder Singh × Start date: 2006 ×
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    Introgression of cotton leaf curl disease resistance from Gossypium armourianum to G. hirsutum
    (Punjab Agricultural University, Ludhiana, 2018) Bhupinder Singh; Rathore, Pankaj
    The present investigation was undertaken to evaluate the performance of interspecific F1 hybrid and backcross derivatives of G. hirsutum and G. armourianum cross for response to cotton leaf curl disease as well as to identify the alien segments in the backcross derivatives. Experimental plant material consisted of G. armourianum Acc. PAU 1, G. hirsutum cv. F 1861 and LH 1556, their F1, BC1F1, BC2F1 derivatives. The interspecific F1hybrid between G. hirsutum and G. armourianum was found to be completely resistant to CLCuD. Two of the four BC1F1 plants were observed to be CLCuD resistant. Out of 84 BC2F1 plants, 43 were observed to be resistant, whereas 41 plants were susceptible to CLCuD. Total genomic DNA of the experimental plant materials was amplified employing virus specific primer pairs. Presence of virus DNA was detected in the American cotton parents as well as CLCuD susceptible backcross derivatives. Of the 100 SSR markers used to detect polymorphism in the parental lines, 52 were polymorphic and were used to identify alien segments in CLCuD resistant BC1F1 Plant 2. Eight markers were amplified in this plant demonstrating introgression from the G. armourianum parent. These primers were used to genotype 84 BC2F1plants. The range of G. armourianum specific alleles in the BC2F1plants was observed to be 38.09 through 75.0%. The introgression of G. armourianum specific chromosomal segments in the BC2F1 plants as revealed by SSR markers was independent of the CLCuD response suggesting that none of the eight primers may be linked to CLCuD resistance. Markers TMB 1271 and NAU 6316 exhibited segregation distortion and were transmitted in higher frequency than expected in the BC2F1 progeny.
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    ThesisItemOpen Access
    Androgenesis response studies in Guava (Psidium guajava L.)
    (Punjab Agricultural University, Ludhiana, 2017) Bhupinder Singh; Mittal, Amandeep
    Development of haploids and doubled haploids through in vitro androgenesis greatly accelerates the process of achieving homozygosity in crop plants including fruit trees. In tree species including Citrus, Papaya, Neem, Mulberry, Custard apple and Apple, androgenesis have been attempted successfully to overcome self in-compatibility barriers so as to raise homozygous trees. In current study we attempted haploid production in three genotypes of guava viz. Allahabad safeda, Purple Guava and Punjab Pink. Anthers with microspores at mid to late uninucleate stage on N6 basal media containing 1% PVP showed maximum callus induction of 16.3±0.27% in Allahabad Safeda followed by 10±0.41% in Punjab Pink and 4.6±0.27% in Purple Guava. The stereomicroscopic evaluation of callus indicated the callus emergence from microspores. The callus induction frequency was enhanced from 1.7±0.27% to 27.7±1.18% by cold (4 ºC) and mannitol (8%) pre-treatment for 3-7 days to unopened stage IV flower buds. 77% calli survived on best regeneration media, nourished with liquid form in the mother test tube without mechanical disturbance for 1 month. One callus exhibited secondary structure on regeneration media. Isolated microspores showed increase in size on MS and N6 media containing 2,4-D (2.5 mg/L) and Kinetin (2.5 mg/L) and few anomalies on MS media fortified with BAP (0.2 mg/L) and Kinetin (0.2mg/L). 2/50 ovaries underwent callusing after 4 months of in vitro culturing on 1% PVP containing basal MS media and callus finally underwent necrosis after one week of sub culturing on MS media fortified with BAP (0.2 mg/L) and Kinetin (0.2 mg/L). Haploid nature of callus can further be evaluated by counting the chromosome number in the cells.
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    ThesisItemOpen Access
    Vegetative And Fruiting Behaviour Of Hard Pear Strains In Relation To Nutrient Status
    (Punjab Agricultural University; Ludhiana, 2006) Bhupinder Singh; Dhillon, W.S.
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    ThesisItemOpen Access
    Response of wheat (Triticum aestivum L.) varieties to different sowing dates and growth regulator
    (Punjab Agricultural University, Ludhiana, 2016) Bhupinder Singh; Hari Ram
    A field experiment entitled “Response of wheat (Triticum aestivum L.) varieties to different sowing dates and growth regulator” was conducted at the Research Farm, Department of Plant Breeding and Genetics, Punjab Agricultural University, Ludhiana during rabi 2015-16. The soil of the experimental field was loamy sand, with neutral in reaction, low in organic carbon and available nitrogen, medium in available potassium but high in available phosphorus. The experiment was conducted in split plot design with combinations of three sowing dates (November 5, November 20 and December 5) and two varieties (WH 1105 and PBW 677) in main plots and four levels of growth regulator (control, water spray, salicylic acid 75 ppm and salicylic acid 100 ppm at booting and early milking) in sub plots, replicated thrice. November 5 and 20 sowing recorded significantly higher growth parameters, tiller density, chlorophyll content, days to different phenological stages, photo-synthetically active radiation (PAR) interception, normalized difference vegetative index (NDVI), growing degree days and economic returns as compared to December 5 sowing. Higher pollen viability was recorded in November 20 sowing however higher proline accumulation was recorded in December 5 sowing. November 5 and 20 recorded similar number of grains per ear, 1000-grain weight, biological yield, grain yield and harvest index. The highest protein content, hectoliter weight and grain hardness was recorded in December 5 sowing. Variety PBW 677 recorded significantly higher growth parameters and days taken to different phenophases than WH 1105. Higher pollen viability, proline content, number of grains per ear, grain yield, harvest index, sedimentation value and net returns were recorded in WH 1105. Salicylic acid (100 ppm) recorded delayed physiological maturity, higher NDVI, lower canopy temperature at 120 DAS, higher pollen viability, proline content, number of grains per ear, 1000-grain weight, grain yield, harvest index and net returns, which was statistically at par with salicylic acid (75 ppm) except proline content. Gluten index and grain appearance score was significantly higher in case of salicylic acid application than control. The variety WH 1105 sown at December 5 recorded similar grain yield as given by variety PBW 677 sown at November 5 and 20. Wheat crop can be grown successfully by planting between November 5 and 20 using variety WH 1105 along with foliar application of salicylic acid (75 or 100 ppm) at booting and early milking.