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    Assessment of extrusion behaviour of white maize
    (Punjab Agricultural University, Ludhiana, 2019) Arshdeep Singh; Baljit Singh
    The present investigation was undertaken to explore the assessment of extrusion behaviour of white maize and their characterization. Response Surface Methodology (RSM) was used to optimize the extrusion process of white maize based extruded products. Central composite rotator design (CCRD) of response surface methodology was explored to evaluate the effects of independent variables, namely feed moisture content (14-18%), screw speed (400-550) and barrel temperature (125-175℃) on product responses specific mechanical energy (SME), bulk density (BD), water absorption index (WAI), water solubility index (WSI). White maize was subjected to extrusion by co-rotating twin screw extruder at varying processing conditions as per the central composite rotatable design. All the processing conditions had significant impact on product responses. Multiple regression equations were obtained to describe the effects of each variable on product responses. The product responses were most affected by change in feed moisture content, barrel temperature and to lesser extent by screw speed. The negative coefficient of moisture and temperature describes that the value of SME declined with increase of these two variables. Barrel temperature was found to elevate the expansion ratio and water solubility index. Optimization studies resulted in 12.50-16.2 % of feed moisture content, 145℃ of barrel temperature and 360-595 rpm of screw speed as optimum variables to produce acceptable white maize snacks. The products were stored for three months and were found acceptable at the end of three months.
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    Diversity analysis and characterization of morpho- anatomical and photosynthetic traits in mandarin (Citrus reticulata Blanco)
    (Punjab Agricultural University, Ludhiana, 2019) Arshdeep Singh; Uppal, Gurteg Singh
    The present study entitled “Diversity analysis and characterization of morpho- anatomical and photosynthetic traits in mandarin (Citrus reticulata Blanco)” was carried out during the years 2017-18 and 2018-19 at Punjab Agricultural University, Ludhiana. Fifteen mandarin genotypes grafted on rough lemon (Citrus jambhiri Lush) rootstocks were evaluated for morphological characters based on IPGRI descriptors. Furthermore, the leaf samples of these genotypes were imaged in scanning electron imaging mode in (model Hitachi S-3400 N) SEM for morpho-anatomical and photosynthetic diversity analysis. The genetic diversity based on these traits was assessed by using DARwin software. The dendrogram grouped genotypes into six different clusters based on morphological characters. The highest similarity coefficient (0.861) was recorded in genotypes Dancy and Wilking. However, Michal showed lowest (0.402-0.539) genetic similarity coefficient with all other mandarin genotypes and these were genetically distinct from each other. The Genotypic Variance (VG) and Phenotypic Variance (VP) of morphological characters were maximum for fruit weight followed by rootstock girth, fruit granulation, scion girth and pollen viability. The heritability was maximum for traits like seed weight, aerola diameter, pedicel length, length of filament, length of style, petal length and vesicle length. Similarly, dendrogram grouped genotypes into five different clusters based on morpho-anatomical and photosynthetic characters. The Genotypic variance (VG) and Phenotypic Variance (VP) were maximum for stomatal density (4531.37 and 7385.39, respectively) followed by number of closed stomata, number of open stomata and palisade parenchyma tissue length. The maximum genetic advance was recorded for stomatal density (108.62). PAU Kinnow 1, a mutant of Kinnow was clubbed in separate cluster due to variation with respect to morpho-anatomical traits. This clearly differentiated the Kinnow from its mutant PAU Kinnow 1. The study clearly indicated presence of moderate level of genetic diversity within mandarin germplasm and this can be exploited for future mandarin breeding programme.