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  • ThesisItemEmbargo
    Water productivity of subsurface drip irrigated spring maizerice-potato cropping system
    (Punjab Agricultural University, Ludhiana, 2022) Amanpreet Kaur; K.B. Singh
    The present study “Water productivity of subsurface drip irrigated spring maize-rice-potato cropping system” was carried out at Punjab Agricultural University, Ludhiana during two consecutive years from October 2018 to October 2020. The experiment was laid out in split plot design with three nitrogen levels {100 % of recommended (N1), 80 % of recommended (N2) and 60% of recommended (N3)} in main plots and seven irrigation regimes {six subsurface drip (at 20 cm depth) and one surface drip in sub plots}. In maize and potato, the sub surface irrigation regimes were (100% of Epan at alternate day ( I1) and two day interval (I2), 80% of Epan at alternate ( I3) and two day interval (I4), 60 % of Epan at alternate day (I5 ) and two day interval (I6) and surface drip at 80% of Epan at alternate day (I7). However, in direct seeded rice the irrigation levels were { 100 % of Epan at alternate day (I1) and two day interval (I2), 80 % of Epan at alternate day(I3) and two day interval (I4), 120 % of Epan at alternate day ( I5) and two day interval (I6) and surface drip at 1.5 times Epan (I7)}. During both years, dry matter accumulation, leaf area index, grain and yield attributed in spring maize were at par with N1 and N2 but significantly higher over N3. Among irrigation levels, grain yield and yield attributes of spring maize was at par with I1 and I3 but significantly higher over all other treatments. Interaction results revealed that grain yield obtained was at par N1I1, N1I3, N1I7 and N2I3. Real water productivity (WPET) of spring maize during both the years was at par with N1I1, N1I3 and N2I3. Results revealed that decreasing irrigation level to 80% and fertilizer nitrogen to 80% under subsurface drip could be best option for obtaining higher yield and water productivity. In direct seeded rice, grain yield and yield attributes decreased significantly with decrease in nitrogen level from recommended dose (N1) during both the years. Grain yield of DSR with N1 was 11.1 and 27.7 % higher over N2 and N3 during 2019 and 10.5 and 34.2 % higher during 2020. During 2020, grain yield of rice was at par with I5 and I7. Grain yield in I7 was 10.6, 14.8, 20.2, 24.2, 3.3 and 6.2 % higher over I1, I2, I3, I4, I5 and I6. Highest WPET (0.92 and 0.96 kgm-3) was obtained with highest level of nitrogen (N1) and decreased significantly at N2 (0.83 kg m-3, 0.87 kg m-3) and at N3 (0.74 kg m-3, 0.73 kg m-3) during 2019 and 2020, respectively. Real water productivity of DSR was highest with I7, was significantly higher over all the other treatments during 2020. In Potato, dry matter accumulation, leaf area index, tuber yield and yield attributes were at par with N1 and N2 but decreased significantly at N3 level. During both the years, tuber yield obtained was at par with I1, I2, I3 but significantly higher over all the other treatments. Highest WPET in potato was obtained with N1I3 treatment combination. Modelling analysis showed DSSAT-CERES -maize, DSSAT- CERES-rice, DSSAT-SUBSTOR-potato performed well in predicting grain yield of spring maize, rice and potato with R2 value 0.90, 0.86,0.95, RMSE 4.6, 3.5, 24.2 qha-1 nRMSE 6.1, 7.0, 7.5 % and NSE 0.8 ,0.6, 0.5 for spring maize, rice and potato respectively.
  • ThesisItemOpen Access
    Collection, identification and domestication of wild edible Agaricales from Punjab
    (Punjab Agricultural University, Ludhiana, 2019) Amanpreet Kaur; Sodhi, H.S.
    Wild edible Agaricales were surveyed and collected during the month of July-September in the years 2015-2018 from five agro-climatic zones of Punjab. Total 127 mushrooms were collected; 34 mushrooms were collected from sub-mountain undulating zone, 19 wild mushrooms were collected from undulating plain zone, 36 mushrooms were collected from central plain zone, 17 from western plain zone, 21 from western zone. The mushrooms collected belong to genera Agaricus, Lepiota/ Macrolepiota, Pleurotus, Poadaxis, Clitocybe. Tissue culture of 92 wild mushrooms were obtained and total cellulases, cellobiase, endo-β-1,4-glucanase, endoxylanase and laccasse were estimated from pure cultures. Total cellulases ranged between 0.01 to 4.36 U/ml, cellobiase between 0.01 to 8.34 U/ml, endo-β-1,4-glucanase between 0.009 to 6.97 U/ml, endoxylanase between 0.28 to 16.18U/ml and laccasse was 0.02-3.74 U/ml. Based on enzyme producing capability, 18 cultures were identified to study linear growth (mm) on complete yeast agar medium (CYM) and potato dextrose agar (PDA) media, different pH range (5-8), temperature range (25°C-28°C). CYM was proved to be the best medium for AMN 05, AMN 71, AMN 132 and PDA was found good for AMN 14 and AMN 60. The pH 7 was proved as best pH for most of the cultures. AMN 14, AMN 39, AMN 52, AMN 54 and AMN 170 showed maximum growth at 30°C. AMN 39 and AMN 202 were successfully domesticated on wheat straw as substrate. These two cultures were subjected to molecular characterization through 18S rRNA sequencing. AMN 39 showed 100% homology with Pleurotus sapidus and AMN 202 showed the same with Pleurotus floridanus. Yield of Pleurotus sapidus (AMN 39) and Pleurotus floridanus (AMN 202) were compared with Pleurotus sajor-caju and Pleurotus florida and found that yield of Pleurotus sapidus (40 kg/q of dry substrate) and Pleurotus floridanus (47 kg/q of dry substrate) was lower than Pleurotus sajor-caju (52 kg/q of dry substrate) and Pleurotus florida (60 kg/q of dry substrate). It was observed through scanning electron microscope that hyphae of Pleurotus florida was seen to be more tightly packed as compared to Pleurotus sapidus (AMN 39) and Pleurotus floridanus (AMN 202). SDS PAGE was performed to know the heterogeneity among the mushroom proteins. AMN 39 had protein with molecular weight of 16.16 KDa and AMN 202 shown to have two proteins with molecular weight of 17.47 KDa and 42.8 KDa.