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20124
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Subject: Zoology × End date: 2012 × Start date: 2012 ×
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    ThesisItemOpen Access
    MUSCLE FATTY ACID PROFILES OF SOME WILD FISH SPECIES AND ENHANCEMENT OF OMEGA-3 FATTY ACIDS THROUGH DIETARY MANIPULATION IN Cyprinus carpio (Linn.)
    (PAU Ludhiana, 2012) Jyotika; H.S., Sehgal
    The study has been conducted on i) seasonal variations in muscle total lipid content (MTLC), fatty acid profiles and proximate composition of two wild fish species, Puntius conchonius (Hamilton-Buchanan) and P. sarana (Hamilton-Buchanan), to determine their nutritional status and ii) enhancement of omega-3 polyunsaturated fatty acids (PUFAs), considered vital for human health, through dietary manipulation in a cultured carp species, Cyprinus carpio (Linn.). Both the wild species were found to be rich in crude protein, which varied between 15.74% to 18.18% in P. conchonius and 15.15% to 18.04% in P. sarana during different seasons. Based on their MTLC (2.36% to 4.89%), both the species could be categorized as lean or low-fat fishes. However, the MTLC was higher and the moisture, ash and carbohydrate contents of the fishes were comparable with many other freshwater fish species. The fishes had good PUFA content. Omega-3 (n-3) PUFAs were 2.78% in P. sarana and 2.21% in P. conchonius. Total n-6 PUFAs were 4.56% in P. sarana and 4.20% in P .conchonius, and the values of n-3/n-6 ratio were 0.73 and 0.60 in P. sarana and P. conchonius, respectively. Linolenic acid formed 1.64% of the total n-3 PUFAs in P. sarana and 1.02% in P. conchonius. EPA constituted 0.31% in P. conchonius and 0.24% in P. sarana and DHA 0.75% in P. sarana and 0.69% in P.conchonius. Stearic acid was the dominant saturated fatty acid (SFA) forming 28.75% of the total SFAs in P. sarana and 28.10% in P. conchonius. The MTLC and n-3 PUFAs of C. carpio (Linn.), a cultured carp species could be enhanced through dietary manipulation. The MTLC could be increased from 1.44% in fish fed traditional diet with no flaxseed oil to 2.83% in fish fed 30% flaxseed oil diet. Similarly, the total n-3 PUFAs could be enhanced from 10.38% in fishes fed 0% flaxseed oil diet for 90 days to 18.67% in fishes fed 30% flaxseed oil diet for 60 days and 30% fish oil diet for 30 days.
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    ThesisItemOpen Access
    EVALUATION OF EUCALYPTUS AND CITRONELLA OILS AS REPELLENTS AGAINST HOUSE RAT, Rattus rattus (Linnaeus)
    (PAU Ludhiana, 2012) Ramandeep Kaur; Neena, Singla
    Experiments were conducted in laboratory pens, maze and store houses using two essential oils namely eucalyptus and citronella oils to study their repellency against house rat, Rattus rattus. In laboratory pens, both oils were applied as paint, as encapsulated wax blocks and as spray each further with three modes of application i.e. daily, once a week, and alternatively in a week at three concentrations 5, 10 and 20%. Repellent effect of the two oils was observed at all the concentrations tested but significantly (P ≤ 0.05) higher effect of eucalyptus oil was in female rats at 20% concentration and of citronella oil in male rats at 10% concentration when applied as spray alternatively. Multichoice experiment conducted in T-maze using 5% eucalyptus and citronella oils as encapsulated wax blocks in choice with no treatment revealed significantly (P ≤ 0.05) higher repellent effect of eucalyptus oil compared to citronella oil in female rats. Bichoice experiment conducted in I-maze using 5% eucalyptus oil as encapsulated wax blocks in choice with no treatment showed significantly (P ≤ 0.05) reduced movements and activities of both male and female rats in treated zone compared to untreated zone. In store house experiments conducted using 5% eucalyptus and citronella oils as encapsulated wax blocks and as spray revealed more percent repellency and control success with eucalyptus oil as encapsulated wax blocks. Present studies thus reveal the efficacy of 5% eucalyptus oil encapsulated in wax blocks as repellent against R. rattus of both sexes.
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    ThesisItemOpen Access
    IDENTIFICATION AND CHARACTERIZATION OF MELOIDOGYNE SPECIES-SPECIFIC PROTEINS
    (Punjab Agricultural University, Ludhiana, 2012) Navjot kaur
    Root knot nematodes (RKNs) of genus Meloidogyne are among the most widely spread parasitic nematodes which attack a wide range of crops and limit their production. The egg masses of Meloidogyne nematodes were cultured on the tomato plants and were identified on the basis of perineal pattern of females by Scanning Electron Microscopy. Only single species of Meloidogyne i.e. M. incognita was identified, indicating that the maintained culture was pure. The crude homogenate/antigen was prepared from the egg masses of established pure culture by homogenization, sonication and centrifugation. Quantitative analysis of prepared homogenate showed an average protein content (mg/gm, wet weight of nematodes) of 128.43 ± 11.10. Protein profile of this crude protein extract by SDS-PAGE indicated a total of seven bands (five major and two minor) observed within a range of molecular weight 27 kDa to 118 kDa. Major band was observed between 50-90 kDa followed by a band between 36-50 kDa. Two minor bands were also observed between 27-36 kDa and 50-90 kDa. Healthy rabbits were immunized with crude protein/antigen of M. incognita egg masses and hyperimmune serum showed significantly higher levels (% of total protein) of Ig from 2.37±0.32 (0 DPI) to 6.98±1.06 (60 DPI), and CIC from 3.48±0.80 (0 DPI) to 12.13±0.56 (60 DPI). Double immunodiffusion technique indicated the production of antibodies, as precipitation line was observed due to the formation of lattices of antigen-antibody complexes. Western blotting resulted in the transfer of single band (lying between 50-90 kDa) from the protein profile of crude antigenic extract of M. incognita analysed on SDS-PAGE on the nitrocellulose membrane probed with anti-Meloidogyne antibodies, thus indicating the antigenic specificity of this protein
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    ThesisItemOpen Access
    HISTOPATHOLOGICAL AND BIOCHEMICAL CHANGES IN LIVER OF RATS FED Bt COTTON SEEDS
    (PUNJAB AGRICULTURAL UNIVERSITY, 2012) KRITIKA SHARMA
    The present study was undertaken to evaluate the effect of feeding Bt and non Bt cotton seeds on the liver of female albino rats. The experimental rats were divided in to three groups viz; control, Bt and non Bt group. They were fed Bt and non Bt cotton seeds at a dose of 2g/kg body weight and control rats received the same amount of water. Body weight of all the rats was taken weekly and feed intake was observed daily for four weeks. One day after the completion of experiment, the rats were sacrificed. The various vital organs i.e. liver, kidney, heart and spleen were excised and weighed. The blood was collected by cardiac puncture in heparinized vials and plasma separated for biochemical estimations. Liver was used for histopathological and biochemical estimations. It was observed that Bt cotton seeds fed rats showed more severe clinical symptoms compared to non Bt cotton seeds fed rats. The net body weight gain significantly increased in both the treated groups as compared to control rats. Average feed intake decreased significantly in non Bt cotton seeds fed rats. The weight of heart decreased significantly in both the groups while the weight of liver significantly increased in Bt cotton seeds fed rats. There was no significant difference in the weight of spleen and kidneys. Histological studies of liver of Bt cotton seeds fed rats showed leukocyte infiltration and irregular shaped hepatocytes nuclei. Non Bt cotton seeds fed rats showed swelling of hepatocytes and loose radial arrangement of hepatocytes around central vein. In the blood plasma proteins, globulin, AST, ALT and ACP increased while AKP decreased significantly in Bt cotton seeds fed rats. The protein and globulin increased significantly in non Bt cotton seeds fed rats. The level of protein, albumin and ACP, AKP, AST and ALT increased in the liver of Bt and non Bt cotton seeds fed rats. Key Words: Bt cotton seeds, non Bt cotton seeds, Albino rats, Liver, Histopathology, Enzymes, Proteins, Albumin, Globulin