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2018 - 201912020 - 20211
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Subject: Microbiology × Author: Navdeep Kaur × Start date: 2017 × Thesis Type: M.Sc ×
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    Upscaling of lignocellulolytic enzyme production from biodigested slurry for increasing biogas production from paddy straw
    (Punjab Agricultural University, Ludhiana, 2021) Navdeep Kaur; Gupta, Urmila
    The objective of the present study was to upscale the process of microbial production of lignocellulolytic enzymes using biodigested slurry as a substrate followed by biogas production from paddy straw using crude lignocellulolytic enzymes. Quantitative screening of the fungal cultures namely Coriolus versicolor MTCC 138, Pleurotus ostreatus MTCC142, Phaenarochaete chrysosporium MTCC 787 and Rhizopus sp indicate that exoglucanase (0.095U/ml), manganese peroxidase (0.305U/ml) and lignin peroxidase (0.437U/ml) activities were maximum in Coriolus versicolor followed by Pleurotus ostreatus. and Phaenarochaete chrysosporium while β-glucosidase activity was maximum in Phaenarochaete chrysosporium. Enzyme production from Coriolus versicolor and Delftia PP4_S3 were statistically optimized by using box-behnken design of response surface methodology (RSM) by Statgraphics software. Coriolus versicolor MTCC 138 produced maximum enzymes at 50% slurry concentration; 3% inoculum; 7 pH; 30˚C temperature and 8 days of incubation. The optimized conditions for maximum enzyme production by Delftia PP4_S3 were: 50% slurry concentration; 1% inoculum; 37˚C temperature and 5 days of incubation. The enzyme production studies were conducted in the field fermentor of 500l capacity using Coriolus versicolor, Pleurotus ostreatus and Delftia PP4_S3. Maximum exoglucanase, β-glucosidase, manganese peroxidase and lignin peroxidase activities were found on 7th day of incubation in Pleurotus ostreatus and Coriolus versicolor while in Delftia PP4_S3 on 4th day of incubation. The crude enzyme produced using BDS was supplemented with paddy straw and was used for biogas production in MS steel biogas plant of 400Kg capacity. The biogas produced by paddy straw supplemented with Delftia crude enzyme was 101m3 as compared to control of 65m3 while biogas produced by paddy straw supplemented with Pleurotus ostreatus crude enzyme was 30m3 where control produced only 27m3. Thus, biodigested slurry is a stable substrate for lignocellulolytic enzymes which can efficiently increase biogas production.
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    ThesisItemOpen Access
    Growth optimisation of Metarhizium anisopliae on cereal grains
    (Punjab Agricultural University, Ludhiana, 2018) Navdeep Kaur; Joshi, Neelam
    Four Metarhizium anisopliae isolates viz. NBAIR (Ma-12), NBAII- 11, MTCC-3872 and PDBC (Ma-13) were evaluated for growth parameter on different media supplemented with different carbon and nitrogen source. The best growth parameters was recorded on media supplemented with lactose as a carbon source @ 30g/l and ammonium sulphate as a nitrogen source @1.00g/l. M. anisopliae on different cereal substrates viz. rice, wheat, sorghum and maize were evaluated for the best growth so as to produce it on large scale. Among all cereal substrate, rice recorded maximum spore count (60.75 X105 CFUg-1) and biomass (3.79g 100ml-1). M. anisopliae isolates NBAII- 11 yielded maximum biomass and spore count and was significantly better than other isolates. Optimum temperature and relative humidity for production of M. anisopliae formulation was 25±2℃ and 90% RH, respectively. M. anisopliae formulation viability on storage at two different temperatures indicated that refrigeration temperature (4±2℃) was better as compared to room temperature (25±2℃) for its viability till six months.