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ThesisItem Restricted Studies on antioxidant and anticancer properties of onion (Allium cepa L.) genotypes(Punjab Agricultural University, Ludhiana, 2020) Inderpal Kaur; Chawla, NeenaThe objectives of the present study were to evaluate the changes in accumulation of antioxidants and the activities of enzymes involved in the synthesis of phenolic compounds in thirty onion genotypes during bulb development stages. The expression of gene (FLS) responsible for the synthesis of flavonol was examined in three selected genotypes, in different plant parts during the bulb development. Study included cytotoxic and apoptotic effects of selected onion extracts on cancer cell lines. Genotypic variation in phenolic content was observed in all onion genotypes during the bulb development. Total phenols, flavonols, odihydroxyphenols, anthocyanin and total antioxidant capacity was observed to be increased from onion bulb initiation stage to bulb maturation stage in most of genotypes, whereas quercetin content was highest reported during bulb developmental stage and then decreased towards maturity. Study showed that enzymes (PAL, FLS and ANS) showed increasing trend from bulb initiation to development stage followed by a decline as the bulb proceeded towards maturation. Correlation and heritability of biochemical parameters were also reported. Three onion genotypes were selected on the basis of phenolic contents and color. Selected genotypes were analysed for the expression study of FLS gene. Study showed maximum relative fold expression of FLS gene in edible portion of plant (leaves and bulb) during bulb development stage. Onion extracts from selected genotypes were prepared in two solvents (ethyl acetate and 80% methanol) to study the potential cytotoxic effects on different cancer cell lines. Combination of ethyl acetate extract of PRO-6 genotype showed significant cytotoxic effects on cancer cell lines.ThesisItem Restricted Biochemical characterization and molecular mapping of stripe rust resistance gene(s) in barley (Hordeum vulgare L.)(Punjab Agricultural University, Ludhiana, 2020) Singla, Prabhjot; Bhardwaj, Rachana D.The study was conducted to appraise the mechanisms of stripe rust resistance in four barley genotypes including three resistant (RD2900, RD2901 and RD2552) and one susceptible (Jyoti) genotypes against different races of Puccinia striiformis f. sp. hordei. Disease reaction showed RD2901 as immune to very resistant followed by RD2552 as immune to resistant and RD2900 as moderately resistant and Jyoti as susceptible against M, G, 57, Q and 24 races. RD2901 possessed higher antioxidant potential in terms of free radical scavenging activities and phenolic compounds under control conditions and maintained sufficiently high on inoculation with different races. Upregulation of NOX, POX, CAT, APX, MDHAR, DHAR, GR, PR proteins, PAL, TAL, GDH and OAT with a concomitant decrease of H2O2 and MDA in RD2901 could also play a vital role in resistance against stripe rust. It also showed maintained levels of β-glucan and increased contents of lignin, proline, glycine betaine and choline. GSH/GSSG significantly declined in Jyoti and increased or remained unaffected in resistant genotypes that might be playing a key role in imparting tolerance. 2-DE showed 36 DEPs in RD2901 under control and infected conditions. Among them, 14 proteins were over expressed and 12 proteins as newly synthesized under infected conditions. Inheritance studies showed that F2 population (RD2901 Jyoti) segregated into 31 resistant and 89 susceptible individuals that fit well to 1:3 which is expected of single recessive resistant gene. BSA indicated that Bmag0500 marker located on 6H chromosome of barley genome was found to be linked with resistant gene.ThesisItem Open Access Manipulation of deamination reactions in the rumen Microorganisms(Department of Biochemistry College of Basic Sciecnes & Humanities, PAU-Ludhiana, 1995) Singh, Kawal Preet; Singh, SudarshanThesisItem Open Access Assembly and secretion of casein micelles in lactating bovines(Department of Biochemistry College of Basic Sciecnes & Humanities, PAU-Ludhiana, 1990) Sodhi, Sandeep; Ahuja, S. PThesisItem Restricted Studies on storage proteins of mongbean (Vigna radiala L.) during germination and seed development(Department of Biochemistry College of Basic Sciecnes & Humanities, PAU-Ludhiana, 1989) Sital, Jagjit Singh; Dhillon, B. SThesisItem Restricted Protection of dietary proteins against rumen microbial fermentation(Department of Biochemistry PAU, Ludhiana, 1983) Kumar, Vijai; Bhatia, I. SThesisItem Restricted Metabolism of dietary lipids in Chicken(Punjab Agricultural University, Ludhiana, 1972) Singh, Jasbir; Singh, JagmohanThesisItem Open Access In vitro studies on the utilization of carbohydrate polymers of tree leves, Grasses and legumes fed to cattle in hilly areas by the mixed culture of the Rumen microflora(Punjab Agricultural University, Ludhiana, 1970) Singh, Kulwant; Bhatia, I. SThesisItem Restricted The effect of the amount and type of vegetable fats in the diet of lactating buffaloes (bes bubalis) on the yield and composition of milk fat(College of Basic Sciences and Humanties PAU, Hissar, 1970) Ahuja, Sat Pal; Bhatia, I. S
