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20211
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Subject: Agricultural Biotechnology × Author: Bhandari, Geetika × Start date: 2021 ×
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    Mapping of some important agronomic traits in pigeonpea
    (Punjab Agricultural University, Ludhiana, 2021) Bhandari, Geetika; Ajinder Kaur
    The present investigation was performed to study the genetics and mapping of the agronomic traits, namely determinate type growth habit, early maturity and short stature in an intraspecific population of pigeonpea derived from cross Pusa 992 (female parent) × AL 2205 (male parent). During kharif 2020, 222 F2 plants were evaluated for growth habit, days to maturity and plant stature along with other agronomic traits. A wide range of variation was observed in F2 population for all traits; 36 plants having early maturing, short statured and determinate type growth habit were harvested separately to raise F2 derived F3 progenies during kharif 2021. These were also evaluated for plant stature, days to maturity and growth habit. The inheritance studies on F2 and F3 populations confirmed monogenic recessive control of determinate type growth habit, early maturity and short plant stature. From a set of 232 SSR primer pairs, 54 markers gave parental polymorphism between two parents. Six bulks for three traits under investigation were made and bulk segregant analysis was carried out on F2 population using 54 polymorphic markers. BSA studies revealed three SSR markers viz. CcM2753, CcM2754 and CcM0268 distinguishing tall/short stature bulk, late/early bulk and IDT/DT bulk, respectively. Single marker analysis was then used to establish marker-trait association for the traits. R2 value of 43.5% demonstrated significant association of marker CcM2753 with short plant stature, while R2 value of 71.8% indicated highly significant association of marker CcM2754 with early maturity. The marker-trait association explained the presence of variability in the phenotype (plant stature and days to maturity) due to variation in genotype. Further, BSA and F2 genotypic data clearly showed the association of marker CcM0268 with determinate type growth habit. Our study highlighted the successful inheritance of agronomic traits viz. determinate type growth habit, early maturity and short plant stature from the male parent AL 2205. The identified molecular markers can be used for marker assisted selection in pigeonpea.